Vegetos
(An International Journal of Plant Research & Biotechnology)
(eISSN: 2229-4473)
Molecular phylogeny of Tabernaemontana spp. (Apocynaceae): insights into correlations
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Research Articles | Published: 17 August, 2026
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Keywords: Apocynaceae, ISSR, Phylogenetic tree, Polymorphism, n Tabernaemontanan
Abstract
Tabernaemontana spp. belonging to the Apocynaceae family, consists of several common and horticultural varieties spanning across the tropical and sub-tropical zones. The genus has previously been taxonomically and pharmacologically explored with limited molecular characterization. Hence, this study focuses on unraveling the genetic diversity and phylogeny among some commonly found species (T. dichotoma, T. coronaria) and horticultural varieties of Tabernaemontana, {T. coronaria (var. variegata, var. dwarf and var. florepleno)} by deploying ISSR markers. Among the 180 bands generated, 92 showed polymorphism implying considerable genetic variation among the taxa. 23 to 38 fragments were amplified per primer indicating genetic variability in the abundance of microsatellites. Phylogenetic tree, constructed on the basis of ISSR banding patterns disclosed low levels of polymorphism among the horticultural varieties of Tabernaemontana. This reflects greater intraspecific genetic affinity among the taxa as compared to their interspecific counterpart. Therefore, the present work highlights the efficacy and virtue of ISSR markers in deducing both intra as well as interspecific relationships among the genus Tabernaemontana. The degree of polymorphism detected from this study creates a diversified genetic base suggesting phylogenetic adaptations among different species and varieties of this taxon. Our findings provide the first ISSR-based molecular evidence of genetic relatedness in Tabernaemontana sp., further opening up avenues for future conservational and molecular breeding practices.
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Author Information
Department of Botany, Dr. Kanailal Bhattacharyya College, Howrah, India