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Mohammed Hanar Jamal, Sulaiman Salam Mahmud, Mahmood Ayub Karim, Halshoy Hawar Sleman, Fattah Omar Ali
Keywords: n Cucumis sativusn , Biostimulants, Productivity, Nutrients, Phenolic compounds, Antioxidants
Microbial and organic plant biostimulants are environmentally friendly options that enhance nutrient absorption, promote plant growth, and improve productivity in terms of both quantity and quality. Thus, an experiment was conducted to evaluate the effectiveness of biostimulants including fungus specifically arbuscular mycorrhizal fungi-Glomus mosseae (AMF-Gs), and marine algae extract (MAE) at three concentrations (600, 1200, and 1800 g/ha), as well as their combination impact on the performance of cucumber plants grown under greenhouse conditions. The results demonstrated that soil inoculation with AMF-Gs and soil application of MAE, both alone and in combination, improved most of the studied characteristics related to root and shoot growth, yield components, and nutraceutical quality of the fruits. Moreover, the most effective treatment was the combination of AMF-Gs inoculation and soil application of MAE at 600 g/ha (T6: AMF-Gs + MAE 1), which maintained a good balance between the plant yield components and the nutraceutical quality of the fruits. Therefore, farmers can utilize these biostimulants to make production more sustainable for consumers, while also maintaining soil health and promoting environmentally friendly agricultural practices.
Abdel-Mawgoud A, Tantaway A, Hafez MM, Habib HA (2010) Seaweed extract improves growth, yield and quality of different watermelon hybrids. Res J Agric Biol Sci 6(2):161–168
Abdulrahman AM, Seed NA, Talib DH, Sharif HB, Khalid JH (2024) Combined impact of climate change and continuous cultivation on greenhouses soil health in Sulaymaniyah Province, Iraq. JKUAS 15(4):9–19. https://doi.org/10.58928/ku24.15402
Ahmed E, Holmström SJJ (2014) Siderophores in environmental research: roles and applications. Microb Biotechnol 7(3):196–208. https://doi.org/10.1111/1751-7915.12117
Ahmed N, Li J, Li Y, Deng L, Deng L, Chachar M, Chachar Z, Chachar S, Hayat F, Raza A (2025) Symbiotic synergy: how arbuscular mycorrhizal fungi enhance nutrient uptake, stress tolerance, and soil health through molecular mechanisms and hormonal regulation. IMA Fungus 16:e144989. https://doi.org/10.3897/imafungus.16.144989
Akatsuka I (1990) Introduction to applied phycology. Balogh Scientific Books
Al-Ealayawi Z, Al-Dulaimy AF (2023) Marine algae and applications to plant nutrition: a review. In: IOP conference series: earth and environmental science. IOP Publishing 1158(4):042004. https://doi.org/10.1088/1755-1315/1158/4/042004
Ali O, Ramsubhag A, Jayaraman J (2019) Biostimulatory activities of Ascophyllum nodosum extract in tomato and sweet pepper crops in a tropical environment. PLoS ONE 14(5):e0216710. https://doi.org/10.1371/journal.pone.0216710
Aliasgharzad N, Shirmohamadi E, Oustan S (2009) Siderophore production by mycorrhizal sorghum roots under micronutrient deficient condition. Soil Environ 28(2):119–123
Asch J, Johnson K, Mondal S, Asch F (2022) Comprehensive assessment of extraction methods for plant tissue samples for determining sodium and potassium via flame photometer and chloride via automated flow analysis. JPNSS 185(2):308–316. https://doi.org/10.1002/jpln.202100344
Ashour M, Hassan SM, Elshobary ME, Ammar GA, Gaber A, Alsanie WF, Mansour AT, El-Shenody R (2021) Impact of commercial seaweed liquid extract (TAM®) biostimulant and its bioactive molecules on growth and antioxidant activities of hot pepper (Capsicum annuum). Plants 10(6):1045. https://doi.org/10.3390/plants10061045
Battacharyya D, Babgohari MZ, Rathor P, Prithiviraj B (2015) Seaweed extracts as biostimulants in horticulture. Sci Hortic 196:39–48. https://doi.org/10.1016/j.scienta.2015.09.012
Carillo P, Kyratzis A, Kyriacou MC, Dell’Aversana E, Fusco GM, Corrado G, Rouphael YJA (2020) Biostimulatory action of arbuscular mycorrhizal fungi enhances productivity, functional and sensory quality in ‘Piennolo del vesuvio’cherry tomato landraces. Agronomy 10(6):911. https://doi.org/10.3390/agronomy10060911
Chesworth J, Stuchbury T, Scaife J, Chesworth J, Stuchbury T, Scaife J (1998) Vegetative growth of plants. Intro to Agric Biochem 343–352
Chojnacka K, Kim S-K (2015) Introduction of marine algae extracts. In: Marine algae extracts, pp 1–14. https://doi.org/10.1002/9783527679577.ch1
Colla G, Cardarelli M, Bonini P, Rouphael Y (2017) Foliar applications of protein hydrolysate, plant and seaweed extracts increase yield but differentially modulate fruit quality of greenhouse tomato. HortScience 52(9):1214–1220. https://doi.org/10.21273/HORTSCI12200-17
Cresser MS, Parsons JW (1979) Sulphuric—perchloric acid digestion of plant material for the determination of nitrogen, phosphorus, potassium, calcium and magnesium. Anal Chim Acta 109(2):431–436. https://doi.org/10.1016/S0003-2670(01)84273-2
Daiswal P (2004) Soil, plant and water analysis kalgani publishers. Ludhiani, New Delhi–Noida (UP), p 264
de Livera J, McLaughlin MJ, Hettiarachchi GM, Kirby JK, Beak DG (2011) Cadmium solubility in paddy soils: effects of soil oxidation, metal sulfides and competitive ions. Sci Total Environ 409(8):1489–1497. https://doi.org/10.1016/j.scitotenv.2010.12.028
Dhankhar N, Kumar J (2023) Impact of increasing pesticides and fertilizers on human health: a review. Mater Today: Proc. https://doi.org/10.1016/j.matpr.2023.03.766
El-Moustaqim K, Sbai SE, El Yousfi Y, Mabrouki J, Hmouni D (2024) New strategy for the advancement of modern agriculture through the use of microalgae as biofertilizers. Euro-Mediterr J Environ Integr 10:1991–2005. https://doi.org/10.1007/s41207-024-00678-x
Fattah OA, Mustafa HA (2023) Effect of mycorrhiza and phosphorus in growth and flowering of daffodil (Narcissus pseudonarcissus L.) growing in calcareous soil. KUJAS 14(2):9–21. https://doi.org/10.58928/ku23.14202
Fusco GM, Nicastro R, Rouphael Y, Carillo PJF (2022) The effects of the microbial biostimulants approved by EU Regulation 2019/1009 on yield and quality of vegetable crops. Foods 11(17):2656. https://doi.org/10.3390/foods11172656
Gerdemann J, Nicolson TH (1963) Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Trans Br Mycol Soc 46(2):235–244
Giovannetti M, Fortuna P, Citernesi AS, Morini S, Nuti MP (2001) The occurrence of anastomosis formation and nuclear exchange in intact Arbuscular Mycorrhizal networks. New Phytol 151(3):717–724. https://doi.org/10.1046/j.0028-646x.2001.00216.x
González-González MF, Ocampo-Alvarez H, Santacruz-Ruvalcaba F, Sánchez-Hernández CV, Casarrubias-Castillo K, Becerril-Espinosa A, Castañeda-Nava JJ, Hernández-Herrera RMJ (2020) Physiological, ecological, and biochemical implications in tomato plants of two plant biostimulants: arbuscular mycorrhizal fungi and seaweed extract. Front Plant Sci 11:999. https://doi.org/10.3389/fpls.2020.00999
Haghighi M, Abdolahipour B (2019) Reducing nitrogen fertilization application in cucumber by mycorrhiza colonization of the plant. IAR 38(1):57–66. https://doi.org/10.22099/iar.2019.5212
Halshoy HS (2025) Growth, yield, and biochemical traits of tomato plants under mycorrhizal inoculation and licorice root extract applications. SSPN 71(2):135–144. https://doi.org/10.1080/00380768.2024.2434832
Halshoy HS, Sadik SK (2025) Impact of training methods and biostimulant applications on sweet pepper (Capsicum annuum) yield and nutritional values: under greenhouse condition. Hortic Plant J 11(1):290–302. https://doi.org/10.1016/j.hpj.2023.06.008
Halshoy HS, Sulaiman SM, Arkwazee H, Mahmood AKJ (2023) Impact of urea supplements and cultivars on yield, nitrate accumulation and some phytochemical properties in lettuce grown under greenhouse conditions. KUJAS 14(2):35–53. https://doi.org/10.58928/ku23.14204
Hines S, van der Zwan T, Shiell K, Shotton K, Prithiviraj B (2021) Alkaline extract of the seaweed Ascophyllum nodosum stimulates Arbuscular Mycorrhizal Fungi and their endomycorrhization of plant roots. Sci Rep 11(1):13491. https://doi.org/10.1038/s41598-021-93035-9
Homulle Z, George TS, Karley AJ (2022) Root traits with team benefits: understanding belowground interactions in intercropping systems. Plant Soil 471(1):1–26. https://doi.org/10.1007/s11104-021-05165-8
Kakbra RF, Sulaiman SM, Tofiq GK. (2023). Impact of biostimulants on nutrients status, yield components and fruit quality of cucumber plants grown under greenhouse conditions. In: 4th international agricultural conference. Publishing House College of Agriculture and Forestry, pp 507–526
Koide RT, Kabir Z (2000) Extraradical hyphae of the mycorrhizal fungus Glomus intraradices can hydrolyse organic phosphate. New Phytol 148(3):511–517. https://doi.org/10.1046/j.1469-8137.2000.00776.x
La Bella S, Consentino BB, Rouphael Y, Ntatsi G, De Pasquale C, Iapichino G, Sabatino L (2021) Impact of Ecklonia maxima seaweed extract and Mo foliar treatments on biofortification, spinach yield, quality and NUE. Plants 10(6):1139. https://doi.org/10.3390/plants10061139
Latif SAA, Mustafa HA (2019) Effect of biofertilizers and carbolizer on growth of gerbera plant (Gerbera jamesonii). Plant Arch 19(1):1733–1754
López-Morales ML, Leos-Escobedo L, Alfaro-Hernández L, Morales-Morales AEJ (2022) Impact of organic fertilizers associated with Mycorrhizae on yield and nutraceutical quality of cucumber. Rev Mex Cienc Agric 13(5):785–798. https://doi.org/10.29312/remexca.v13i5.2868
Lucini L, Rouphael Y, Cardarelli M, Bonini P, Baffi C, Colla GJ (2018) A vegetal biopolymer-based biostimulant promoted root growth in melon while triggering brassinosteroids and stress-related compounds. Front Plant Sci 9:472. https://doi.org/10.3389/fpls.2018.00472
Lüthje S, Böttger M (1995) On the function of a K-type vitamin in plasma membranes of maize (Zea mays L.) roots. Mitt Inst Allg Bot Hamb 25:513
Mahmood AK, Sulaiman SM, Arkwazee HAH (2021) Evaluation of yield and fruit quality of newly introduced cherry tomato cultivars under high tunnel conditions. EJAS 13(4):35–45
Mohammed HA, Aljabary AMAO, Halshoy HS, Hama JR, Rashid HA, Rashid HW (2023) Soil-borne microbes, natural stimulants, and post-harvest treatments alter quality and phytochemicals of tomato fruit. Int J Veg Sci 29(6):544–556. https://doi.org/10.1080/19315260.2023.2272838
Mohammed NT, Halshoy HS, Saed NF, Ali HWR, Mohammed NI, Ali SMJ (2024) Impact of inorganic fertilizer doses on growth, yield, physical and chemical components of broccoli plants. JKAS 11(4):57–72. https://doi.org/10.59658/jkas.v11i4.2791
Mohammed NT, Halshoy HS, Saed NF, Ahmed SM, Amen HRH, Ali HWR, Ali SM, Ibrahim AS (2025) Mycorrhizal fungi and chicken manure: a sustainable strategy for cucumber plant productivity and quality. Vegetos. https://doi.org/10.1007/s42535-025-01173-6
Mok MC (2019) Cytokinins and plant development—an overview. Cytokinins 155–166
Mukherjee PK, Nema NK, Maity N, Sarkar BK (2013) Phytochemical and therapeutic potential of cucumber. Fitoterapia 84:227–236. https://doi.org/10.1016/j.fitote.2012.10.003
Nanda S, Kumar G, Hussain S (2022) Utilization of seaweed-based biostimulants in improving plant and soil health: current updates and future prospective. IJEST 19(12):12839–12852. https://doi.org/10.1007/s13762-021-03568-9
Olsen S, Sommers E (1982) Phosphorus soluble in sodium bicarbonate. Methods of Soil Analysis 2:404–430
Rasouli F, Amini T, Asadi M, Hassanpouraghdam MB, Aazami MA, Ercisli S, Skrovankova S, Mlcek JJ (2022) Growth and antioxidant responses of lettuce (Lactuca sativa L.) to arbuscular mycorrhiza inoculation and seaweed extract foliar application. Agronomy 12(2):401. https://doi.org/10.3390/agronomy12020401
Rouphael Y, Cardarelli M, Di Mattia E, Tullio M, Rea E, Colla G (2010) Enhancement of alkalinity tolerance in two cucumber genotypes inoculated with an Arbuscular Mycorrhizal biofertilizer containing Glomus intraradices. Biol Fertil Soils 46(5):499–509. https://doi.org/10.1007/s00374-010-0457-9
Rouphael Y, Franken P, Schneider C, Schwarz D, Giovannetti M, Agnolucci M, Pascale SD, Bonini P, Colla G (2015) Arbuscular mycorrhizal fungi act as biostimulants in horticultural crops. Sci Hortic 196:91–108. https://doi.org/10.1016/j.scienta.2015.09.002
Ryan J, Estefan G, Rashid A (2001) Soil and plant analysis laboratory manual. ICARDA
Sagar A, Rathore P, Ramteke PW, Ramakrishna W, Reddy MS, Pecoraro LJM (2021) Plant growth promoting rhizobacteria, arbuscular mycorrhizal fungi and their synergistic interactions to counteract the negative effects of saline soil on agriculture: key macromolecules and mechanisms. Microorganisms 9(7):1491. https://doi.org/10.3390/microorganisms9071491
Schenck NC (1990) Manual for the identification of VA mycorrhizal fungi. Synergistic Publications Gainesville, p 286
Shukla PS, Mantin EG, Adil M, Bajpai S, Critchley AT, Prithiviraj BJ (2019) Ascophyllum nodosum-based biostimulants: sustainable applications in agriculture for the stimulation of plant growth, stress tolerance, and disease management. Front Plant Sci 10:462648. https://doi.org/10.3389/fpls.2019.00655
Siddique KHM, Chen YL, Rengel Z (2015) Efficient root system for abiotic stress tolerance in crops. Procedia Environ Sci 29:295. https://doi.org/10.1016/j.proenv.2015.07.269
Stachniuk A, Szmagara A, Stefaniak EA (2018) Spectrophotometric assessment of the differences between total nitrate/nitrite contents in peel and flesh of cucumbers. Food Anal Methods 11(10):2969–2977. https://doi.org/10.1007/s12161-018-1274-2
Stockinger H, Krüger M, Schüßler AJNP (2010) DNA barcoding of Arbuscular Mycorrhizal Fungi. New Phytol 187(2):461–474. https://doi.org/10.1111/j.1469-8137.2010.03262.x
Sulaiman S, Sadiq S (2020) Influence of greenhouse shading and different nutrient management practices on root colonization by AMF and plant root architecture of tomato (Solanum lycopersicum L.). Plant Arch 20:1929–1939
Tabart J, Kevers C, Sipel A, Pincemail J, Defraigne J-O, Dommes J (2007) Optimisation of extraction of phenolics and antioxidants from black currant leaves and buds and of stability during storage. Food Chem 105(3):1268–1275. https://doi.org/10.1016/j.foodchem.2007.03.005
Takatsuka H, Umeda M (2014) Hormonal control of cell division and elongation along differentiation trajectories in roots. J Exp Bot 65(10):2633–2643. https://doi.org/10.1093/jxb/ert485
Talabani SK, Mahmood CH, Halshoy HS, Braim SA, Hama JR (2025) Optimizing spinach germination and quality with Azotobacter inoculation and synthetic nitrogen fertilization. Cogent Food & Agriculture 11(1):2562176. https://doi.org/10.1080/23311932.2025.2562176
Thirumaran G, Arumugam M, Arumugam R, Anantharaman P (2009) Effect of seaweed liquid fertilizer on growth and pigment concentration of Cyamopsis tetrogonolaba (L) Taub. Am Eurasian J Agron 2(2):50–56
Trejo Valencia R, Sánchez Acosta L, Fortis Hernández M, Preciado Rangel P, Gallegos Robles MÁ, Antonio Cruz RdC, Vázquez Vázquez CJA (2018) Effect of seaweed aqueous extracts and compost on vegetative growth, yield, and nutraceutical quality of cucumber (Cucumis sativus L.) fruit. Agronomy 8(11):264. https://doi.org/10.3390/agronomy8110264
Verma S, Sharma A, Kumar R, Kaur C, Arora A, Shah R, Nain L (2015) Improvement of antioxidant and defense properties of tomato (var. Pusa Rohini) by application of bioaugmented compost. Saudi J Biol Sci 22(3):256–264. https://doi.org/10.1016/j.sjbs.2014.11.003
Wilke B-M (2005) Determination of chemical and physical soil properties. In: Margesin R, Schinner F (eds) Monitoring and assessing soil bioremediation. Springer, Berlin Heidelberg, pp 47–95. https://doi.org/10.1007/3-540-28904-6_2
Zhang J, Feng S, Yuan J, Wang C, Lu T, Wang H, Yu CJ (2021) The formation of fruit quality in Cucumis sativus L. Front Plant Sci 12:729448. https://doi.org/10.3389/fpls.2021.729448
Zhang J, Gu X, Yan W, Lou L, Xu X, Chen XJF (2022) Characterization of differences in the composition and content of volatile compounds in cucumber fruit. Foods 11(8):1101. https://doi.org/10.3390/foods11081101
Horticulture Department, College of Agricultural Engineering Sciences, University of Sulaimani, Sulaymaniyah, Iraq