Vegetos
(An International Journal of Plant Research & Biotechnology)
Optimization of extraction of biosurfactant from the fruits of Balanites aegyptiaca using sonication through analysis of variance
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Singh Samar Pratap, Singh Asmita, Saini Mahesh Kumar, Prasad Jagdish, Chauhan Manmohan Singh, Singh Divyanshu, Gour Vinod Singh
Research Articles | Published: 28 April, 2025
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Keywords: Relative surface tension, Duncan’s multiple range test, Process optimization, pH, Temperature
Abstract
The mesocarp of the fruit of Balanites aegyptiaca has biosurfactant activity. This biosurfactant retains its surfactant activity at varying temperatures, pH, and salinity conditions. However, till now the surfactant extraction procedure for B. aegyptiaca has not been optimized. Therefore, in the present study experiments have been designed to study the effects of duration of sonication, pH, and temperature on the extraction of surfactant which has been evaluated in terms of relative surface tension. The results revealed that the duration of sonication (10, 20, and 30 min) has no significant effect while temperature and pH significantly influence the extraction of the surfactant. From the statistical analysis of data (Analysis of Variation), it can be concluded that we can extract the maximum amount of biosurfactant at pH 7 and 35oC temperatures by sonicating the suspension of mesocarp powder for 10 min.
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Author Information
Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur, India