Optimizing nutrient media conditions for continuous production of shoot biomass enriched in major medicinal constituents, amarogentin and mangiferin of endangered medicinal herb, Swertia chirayita

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00464-6
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Keywords: Callus, Differentiation, Metabolites, Regeneration, Shoots


Biosynthesis and accumulation of Amarogentin and Mangiferin from shoot culture of endangered herb Swertia chirayita helped in rescuing its natural population along with continuous production of quality rich herbal material. Although, presence of Amarogentin and Mangiferin had already been reported, but such studies did not elaborate the significant developmental stages at two varying temperature (15 ± 1 °C and 25 ± 1 °C) in shoot cultures of S. chirayita. Different developmental stages involved throughout from callus induction to complete regeneration of plant by using shoot cultures of S. chirayita, reveal different amounts of significant medicinal compounds having high pharmacological importance like bearing anti-diabetic and anti-cancerous properties. So in the present study, different developmental stages i.e. plant segment as leaf disc explants, initiation of callus formation, callus mass development, shoots primordial, manifold shoot formation and shoot elongation with complete growth were explored for accumulation of Amarogentin and Mangiferin. The Amarogentin content was 4.72 µg/mg at 15 ± 1 °C and 4.41 µg/mg at 25 ± 1 °C whereas Mangiferin content was 15.54 µg/mg at 15 ± 1 °C and 9.70 µg/mg at 25 ± 1 °C in leaf discs provided with the medium MS + 2,4D = 1 mg/L, 6BAP = 0.5 mg/L, TDZ = 0.5 mg/L, respectively. The accumulation of Amarogentin and Mangiferin started from callus cultures differentiating into shoots and reached to the detectable amount equivalent to actual leaf explants in fully grown shoots with content of 5.79 µg/mg at 15 ± 1 °C and 5.35 µg/mg at 25 ± 1 °C whereas 15.56 µg/mg at 15 ± 1 °C and 13.15 µg/mg at 25 ± 1 °C provided with the medium MS + IBA = 3 mg/L, KN = 1 mg/L, respectively. Maximum accumulation of bioactive compounds was observed in ≈3 months old in-vitro grown shoots at 15 ± 1˚ C wherein, the content of Amarogentin was ≈8.51 folds higher and Mangiferin was ≈4.09 folds higher than the ≈3 months old green house grown shoots. So, the in-vitro raised shoots of S. chirayita enriched with marker medicinal compounds would be utilized as ready to use raw material for pharmaceutical industries for herbal drug formulations and can be utilized to transfer under natural habitats for conserving its diminishing population.


Aerts R, De Luca V (1992) Phytochrome is involved in the light regulation of vindoline biosynthesis in Catharanthus. Plant Physiol 100:1029–1032

Alam KD, Ali MS, Parvin S, Mahjabeen S, Akbar MA, Ahamed R (2009) In-vitro antimicrobial activities of different fractions of Swertia chirata ethanolic extract. Pak J Biol Sci 12:1334–1337

Banerjee S, Sur TP, Das PC, Sikdar S (2000) Assessment of the anti-inflammatory effects of Swertia chirata in acute and chronic experimental models in male albino rats. Indian J Pharmacol 32:21–24

Dar A, Faizi S, Naqvi S, Sadia R, Rehman ZU, Ali M, Firdous S, Moin ST (2005) Analgesic and antioxidant activity of mangiferin and its derivatives: the structure activity relationship. Biol Pharm Bull 28:596–600

Das SC, Bhadra S, Roy S, Saha SK, Islam MS, Bachar SC (2012) Analgesic and anti-inflammatory activities of ethanolic root extract of Swertia chirata (Gentianaceae). JordanJ Biol Sci 5:31–36

Disasa D, Cheng L, Manjoor M, Liu Q, Wang Y, Xiang L, Qi J (2020) Amarogentin from gentiana rigescens franch exhibits antiaging and neuroprotective effects through antioxidative stress. Oxid Med Cellular Longev 2020:1–15

Du M, Wen G, Jin J, Chen Y, Cao J, Xu A (2017) Mangiferin prevents the growth of gastric carcinoma by blocking the PI3K-Akt signalling pathway. Anti Cancer Drugs 29:167–175

García D, Escalante M, Delgado R, Ubeira FM, Leiro J (2003) Anthelminthic and antiallergic activities of Mangifera indica L. stem bark components Vimang and mangiferin. Phytother Res 17:1203–1208

Grzegorczyk I, Matkowski A, Wysokin´ska H (2007) Antioxidant activity of extracts from in-vitro cultures of Salvia officinalis L. Food Chem 104:536–541

Imran M, Arshad MS, Butt MS, Kwon JH, Sultan MT (2017) Mangiferin: a natural miracle bioactive compound against lifestyle related disorders. Lipids Health Dis 16:84–101

Kar A, Choudhary BK, Bandyopadhyay NG (2003) Comparative evaluation of hypoglycaemic activity of some Indian medicinal plants in alloxan diabetic rats. J Ethnopharmacol. 84:105–108

Kar P, Kumar V, Vellingiri V, Sen A, Jaishee N, Anandraj A, Malhotra H, Roy A, Subramaniam MD (2020) Anisotine and amarogentin as promising inhibitory candidates against SARS-CoV-2 proteins: a computational investigation. J Biomol Struct Dyn 40:1–10

Karan M, Vasisht K, Handa SS (1999) Morphological and chromatographic comparison of certain Indian species of Swertia. J Med Aromat PlantSci 19:995–963

Kaur P, Pandey DK, Gupta RC, Dey A (2019) Simultaneous microwave assisted extraction and HPTLC quantification of mangiferin, amarogentin, and swertiamarin in Swertia species from western Himalayas. Ind Crops Prod 132:449–459

Kumar V, Staden JV (2016) A review of Swertia chirayita (Gentianaceae) as a traditional medicinal plant. Front Pharmacol 6:1–14

Kumar V, Chauhan RS, Sood H (2013) In-vitro production and efficient quantification of major phyto pharmaceuticals in an endangered medicinal herb, Swertia chirata. Int J Biotechnol Bioeng Res 4:495–506

Kumar V, Singh S, Bandopadhyay R, Sharma M, Chandra S (2014) In vitro organogenesis secondary metabolite production and heavy metal analysis in Swertia chirayita. Cent Eur J Biol 9:686–698

Kumar P, Pal T, Sharma N, Kumar V, Sood H, Chauhan RS (2015) Expression analysis of biosynthetic pathway genes vis-a`-vis podophyllotoxin content in Podophyllum hexandrum Royle. Protoplasma. https://doi.org/10.1007/s00709-015-0757-x

Laxmi A, Siddhartha S, Archana M (2011) Antimicrobial screening of methanol and aqueous extracts of Swertia chirata. Int J Pharm Pharm Sci 3:142–146

Luczkiewicz P, Kokotkiewicz A, Dampc A, Luczkiewicz M (2014) Mangiferin: a promising therapeutic agent for rheumatoid arthritis treatment. Med Hypotheses 83:123–135

Medda S, Mukopadhyaya S, Basu MK (1999) Amarogentin, a secoiridoid glycoside, activates AMP- activated protein kinase (AMPK) to exert beneficial vasculometabolic effects. J Antimicrob Chemother 44:791–794

Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497

Niu HS, Chao PC, Ku PM, Niu CS, Lee KS, Cheng JT (2016) Amarogentin ameliorates diabetic disorders in animal models. Naunyn Schmiedebergs Arch Pharmacol 389:1215–1223

Phoboo S, Pinto MDS, Barbosa ACL, Sarkar D, Bhowmik PC, Jha PK (2013) Phenolic-linked biochemical rationale for the anti-diabetic properties of Swertia chirayita (Roxb.exFlem.). Phytother Res 27:227–235

Potunuru UR, Priya KV, Varsha MKNS, Mehta N, Chandel S, Dixit M (2019) Amarogentin, a secoiridoid glycoside, activates AMP- activated protein kinase (AMPK) to exert beneficial vasculo-metabolic effects. BBA Gen Sub 1863:1270–1282

Pradhan JK, Kumar V, Sood H, Chauhan RS (2015) Contents of therapeutic metabolites in Swertia chirayita correlate with the expression profiles of multiple genes in corresponding biosynthesis pathways. Phytochemistry 116:38–47

Rajendran P, Rengarajan T, Nishigaki I, Eakmbaram G, Shakthisekaran D (2014) Potent chemopreventive effect of mangiferin on lung carcinogenesis in experimental Swiss albino mice. J Cancer Res Ther 10:1033–1039

Ray S, Jha S (2001) Production of with a ferin A in shoot cultures of Withania somnifera dunal. Planta Med 67:432–437

Saha P, Mandal S, Das A, Das S (2006) Amarogentin can reduce hyper proliferation by down regulation of Cox-II and upregulation of apoptosis in mouse skin carcinogenesis model. Cancer Lett 244:252–259

Sekiguchi Y, Mano H, Nakatani S, Shimiju J, Ebata M, Wada M (2017) Mangiferin positively regulates osteoblast differentiation and suppresses osteoclast differentiation. Mol Med Rep 16:1382–1332

Sharma V, Srivastava N, Kamal B, Dobriyal AK, Jadon VS (2011) Swertia chirayita: a review to revitalize its importance in pharmaceutical arena. J Pharm Res 4:1784–1787

Sharma N, Chauhan RS, Sood H (2016) Discerning picroside-I biosynthesis via molecular dissection of in-vitro shoot regeneration in Picrorhiza kurroa. Plant Cell Rep 35:1601–1615

Shrivastava N, Patel T, Srivastava A (2006) Biosynthetic potential of in-vitro grown callus cells of Cassia senna L var. senna. Curr Sci 90:1472–1473

Shubham BU, Mathur A (2016) Isolation and identification of amarogentin as an antihelminthic compound in Swertia chirayta. J Chem Pharm Res 8:1374–1381

Shukla S, Bafna K, Sundar D, Thorat SS (2014) The bitter barricading of prostaglandin biosynthesis pathway: understanding the molecular mechanism of selective cyclooxygenase-2 inhibition by amarogentin, a secoiridoid glycoside from swertia chirayita. PLoS ONE 9:1–15

Sood H, Chauhan RS (2009) Biosynthesis and accumulation of a medicinal compound, Picroside-1 in cultures of Picrorhiza kurroa Royle ex Benth. Plant Cell Tissue Organ Cult 100:113–117

Tanaka N, Takao M, Matsumoto T (1995) Vincamine production in multiple shoot culture derived from hairy roots of Vinca minor. Plant Cell Tissue Organ Cult 41:61–64

Thiem B, Krawczyk A (2003) Ellagic acid in in-vitro cultures of Rubus chamaemorus L. Herba Pol 49:202–209

Verma H, Patil PR, Kolhapure RM, Gopal KV (2008) Antiviral activity of the Indian medicinal plant extract Swertia chirata against herpes simplex viruses study by in-vitro and molecul arapproach. Indian J Med Microbiol 26:322–326

Verma VK, Sarwa KK, Kumar A (2013) Comparison of hepato protective activity of Swertia chirayita and Andrographis paniculata plant of north east India against CCl4 induced hepatotoxic rats. J Pharm Res 7:647–653

Yen TL, Lu WJ, Lien LM, Thomas PA, Lee TY, Chiu HC, Sheu JR, Lin KH (2014) Amarogentin, a secoiridoid glycoside, abrogates platelet activation through PLCγ2-PKC and MAPK pathways. Biomed Res Int 2014:1–9

Zeng Z, Lin C, Wang S, Wang P, Xu W, Ma W, Wang J, Xiang Q, Liu Y, Luo Y, Liu SL, Liu H (2020) Suppressive activities of mangiferin on human epithelial ovarian cancer. Phymed 76:153–167



The authors are pleased to the JUIT, Waknaghat, India for facilitating suitable lab conditions to carry out this experiment.

Author Information

Gupta Rolika
Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan, India
Sood Hemant
Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan, India