Phenolics compounds, evaluation of Alpha-amylase, alpha‐glucosidase inhibitory capacity and antioxidant effect from Globularia alypum L

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Research Articles | Published:

E-ISSN: 2229-4473.
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DOI: 10.1007/s42535-021-00211-3
First Page: 477
Last Page: 484
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Keywords: Globularia alypum , Phenolics compounds, Oxidative stress, Diabetes mellitus, RP-HPLC-PDA


Abstract

The aim of this study was to evaluate in vitro antioxidant and inhibitory Alpha-amylase and Alpha-glucosidase activities of fractions from Globularia alypum. Phenolic and flavonoids contents were estimated by Folin–Ciocalteu and aluminum chloride colorimetric methods, respectively. Identification of phenolic constituents was carried out by RP-HPLC–PDA method for diethyl ether and ethyl acetate fractions. Antioxidant activity was assessed by DPPH, FRAP and antihemolysis assays. Antidiabetic activity was evaluated as the first report on in vitro inhibitory effect of fractions on key diabetes enzymes: Alpha-amylase and Alpha-glucosidase. Fractions from G. alypum revealed high enzyme inhibitory activities and potent antioxidant effect. They showed significant antihemolytic results (HT50 from 194 ± 4.86 to 289 ± 5.67 min). Diethyl ether fraction presented the highest amounts in phenolic (331.88 ± 17.80 µg GAE/mg DF) and flavonoids (223.46 ± 2.37 µg CE/mg DF). HPLC analysis revealed the presence of gallic acid, p-coumaric acid, rutin, naringenin and quercetin. It exhibited the highest antioxidant activity assayed by DPPH (IC50 = 20.54 ± 0.48 µg/ml) and FRAP (EC50 = 283.7 ± 82.59 µg/ml). It showed the best inhibitory effect on Alpha-amylase (IC50 = 0.57 ± 0.05 mg/ml) and Alpha-glucosidase (IC50 = 0.52 ± 0.02 mg/ml). Globularia alypum is rich in phenolics and flavonoids. It is a promising source of potent natural antioxidants. It is suggested as an effective plant material for antidiabetic drugs.

Globularia alypum , Phenolics compounds, Oxidative stress, Diabetes mellitus, RP-HPLC-PDA


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Author Information

Laboratoire Antibiotiques Antifongiques : Physico-chimie, Synthèse et Activité Biologique, Faculté des sciences de la nature et de la vie et des sciences de la terre et de l’univers, Université de Tlemcen, Tlemcen, Algérie