Vegetos
(An International Journal of Plant Research & Biotechnology)
Phenotypic diversity and trait association in cluster bean (Cyamopsis tetragonoloba)
Research Articles | Published: 27 March, 2023
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
First Page: 329
Last Page: 340
Views: 1541
Keywords:
Cluster bean, Genetic variability, Dendrogram, Principal component analysis, Phenotype, Cluster analysis
Abstract
Cluster bean (Cyamopsis tetragonoloba) is an important industrial crop in arid and semi-arid regions. The present investigation reports phenotypic variability in 219 guar accessions based on 16 morphological traits for two consecutive years. The ANOVA showed significant differences among accessions for all the traits. The high estimates of GCV, PCV, h2b and GA were observed for days to 50% flowering, plant height, number of pods/plant and fruit yield/plant. The seed yield/ plant (SYP) showed significantly positive correlation with the number of pods/cluster, the number of pods/ plant, fruit yield/ plant, and the number of seeds/ plant in both years. Principal component analysis showed that the first three principal components accounted for more than 75% of the total variation in both years. The hierarchical clustering by Ward’s minimum variance grouped all 219 accessions into nine clusters. The cluster analysis revealed that cluster VII was the largest one and contained the maximum accessions in both years. Most of the clusters contained common accessions and cluster IX had the maximum common accessions. The cluster mean revealed that the cluster VIII was characterized by taller plant height, less number of branches, large number of pods/ plant, large pod length and pod width and high fruit yield of the accessions. The present study determines some promising diverse accessions with high potentials that could be useful for further genetic improvement programme in cluster bean.
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References
Ahmad MB, Hamed RA, Ali ME, Hassan AB, Babiker EE (2006) Proximate composition, antinutritional factors and protein fractions of guar gum seeds as influenced by processing treatments. Pakistan J Nutr 5:481–484
Allard RW (1999) Principles of plant breeding, 2nd edn. Wiley, New York
Bewal S, Purohit J, Kumar A, Khedasana R, Rao SR (2009) Cytogenetical investigations in colchicine induced tetraploids of Cyamopsis tetragonoloba L. Czech J Genet plant Breed 45:143–154
Boghara MC, Dhaduk HL, Kumar S, Parekh MJ, Patel NJ, Sharma R (2016) Genetic divergence, path analysis and molecular diversity analysis in cluster bean (Cyamopsis tetragonoloba L. Taub.). Ind Crops Prod 89:468–477
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Burruano BT, Schnaare RL, Malamud D (2002) Synthetic cervical mucus formulation. Contraception 66:137–140
Dabholkar AR (1992) Elements of biometrical genetics. Concept publishing company, New Delhi
Dhugga KS, Barreiro R, Whitten B, Stecca K, Hazebroek J, Randhawa GS, Dolan M, Kinney AJ, Tomes D, Nicholas S (2004) Guar seed beta-mannan synthase is a member of the cellulose synthase super gene family. Science 303:363–366
Frias ACD, Sgarbieri VC (1999) Guar gum effects on food intake, blood serum lipids and glucose levels of wistar rats. Plant Foods Human Nutrition 53:15–28
Girish MH, Gasti VD, Mastinoli AB, Thammaiah N, Shantappa T, Mulge R, Kerutagi M (2012) Genetic divergence studies in cluster bean genotypes (Cyamopsis tetragonoloba (L.) Taub). Karnataka J Agricultural Sci 25:245–247
Gresta F, Avola G, Cannavo S, Santonoceta C (2018) Morphological, biological, productive and qualitative characterization of 68 guar (Cyamopsis tetragonoloba (L.) Taub) genotypes. Ind Crops Prod 114:98–107
Gresta F, Sortino O, Santonoceto C, Issi L, Formantici C, Galante YM (2013) Effects of sowing times on seed yield protein and galactomannans content of four varieties of guar (Cyamopsis tetragonoloba L.) in a Mediterranean environment. Industrial crop and products 41:46–52
Hassan SM, Aqil AA, Attimard M (2013) Determination of crude saponin and flavonoids content in guar meal. Advancement in medicinal plant research 1:24–28
Hosobuchi C, Rutanassee L, Bassin SL, Wong ND (1999) Efficacy of acacia, pectin and guar gum- based fiber supplementation in the control of hypercholesterolemia. Nutritional Res 19:643–649
Hymowitz T, Upadhya MD (1963) The chromosome number of Cyamopsis serrata sching. Curr Sci 32:427–428
Johnson HW, Robinson HF, Comstock RE (1955) Estimates of genetic and environmental variability in soybeans. Agron J 47:314–318
Jukanti A, Bhatt R, Sharma R, Kalia R (2015) Morphological, agronomic, and yield characterization of cluster bean (Cyamopsis tetragonoloba L.) germplasm accessions. J Crop Sci Biotechnol 18:83–88
Khalid MAR, Akhtar LH, Minhas R, Bukhari SJ, Zubair M, Iqbal MA (2017) Screening of guar accessions [Cyamopsis tetragonoloba (L.) Taub] for higher yield potential under irrigated conditions. Afr J Agric Res 12:2788–2794
Kumar D, Singh NB (2002) Guar in India. Scientific Publishers, Jodhpur, India
Kumar V, Ram RB, Yadav RK (2014) Genetic diversity in cluster bean [Cyamopsis tetragonoloba (L.) Taub.] For morphological characters. Indian J Sci Technol 7:1144–1148
Lee JT, Appleton SC, Haq AU, Bailey CA, Cartwright AL (2004) Quantitative measurement of negligible trypsin inhibitors activity and nutrient analysis of guar mean fraction. J Agricultural Food Chem 52:6492–6495
Mahalanobis PC (1936) On the generalized distance in statistics. Proceedings of the National Institute of Science of India 2: 49–55
Manivannan A, Anandakumar CR, Ushakumari R, Dahiva GS (2015) Genetic diversity of guar genotypes [Cyamopsis tetragonoloba (L.) Taub.] Based on agro-morphological traits. Bangladesh J Bot 44:59–65
Morris JB (2010) Morphological and reproductive characterization of guar (Cyamopsis tetragonoloba) genetic resources regenerated in Georgia. USA Genetic resources and crop evolution 57:985–993
Mudgil D, Barak S, Khatkar BS (2014) Guar gum: processing properties and food applications – a review. J Food Sci Technol 51:409–418
Murthy SN, Hiremath SRR, Paranjothy KLK (2004) Evaluation of carboxymethyl guar films for the formulation of transdermal therapeutic system. Int J Pharm 272:11–18
Pabal A, More V, Sukhadev K, Parimal V, Bhausaheb G (2013) Molecular characterization of cluster bean (Cyamopsis tetragonoloba) cultivars using PCR-based molecular markers. Int J Adv Biotechnol Res 4:158–166
Panse VG, Sukhatme PV (1967) Statistical methods for agricultural workers. Indian council of agricultural research, New Delhi 381
Pathak R, Singh SK, Singh M, Henry A (2011) Molecular assessment of genetic diversity in cluster bean (Cyamopsis tetragonoloba) genotypes. J Genet 89:243–246
Patil DV (2014) Genetic variability and sowing dates effect of cluster bean (Cyamopsis tetragonoloba L. Taub) genotypes in semi-arid region of Maharashtra, India. Plant archives. 14: 1–6
Rai SP, Dharmatti PR, Shashidhar TR, Patil RV, Patil BR (2012) Genetic variability studies in clusterbean [Cyamopsis tetragonoloba (L.) Taub]. Karnataka journal of. Agric Sci 25:108–111
Rao CR (1952) Advanced statistical methods in biometrical research. Wiley, New York, p 45
Sharma A, Mishra S, Garg G (2013) Molecular characterization and genetic relationships among cluster bean genotypes based on RAPD Analysis. Res J Pharm Biol Chem Sci 1:8–17
Sharma P, Kumar V, Venkat RK, Tiwari K (2014) A set of SCAR markers in cluster bean (Cyamopsis tetragonoloba L. Taub) genotypes. Adv bioscience Biotechnol 5:131–141
Shyale S, Chowdary K, Krishnaiah Y, Bhat NK (2006) Pharmacokinetic evaluation and studies on the clinical efficacy of guar gum-based oral drug delivery systems of albendazole and albendazole-β-cyclodextrin for colon targeting in human volunteers. Drug Dev Res 67:154–165
Singh RK, Chaudhary BD (1985) Biometrical methods in quantitative genetic analysis. Kalyani Publishers, New Delhi, India
Sultan M, Rabbani MA, Shinwari KZ, Masood (2012) Phenotypic divergence in guar (Cyamopsis tetragonoloba) landrace genotype of Pakistan. Pak J Bot 44:203–210
Wankhade RS, Kale VS, Nagre PK, Patil RK (2017) Genetic divergence studies in cluster bean genotypes. Legume Res 40:811–817
Acknowledgements
The Authors would like to thank the Director, CSIR-National Botanical Research Institute, Lucknow, for providing the facilities to carry out the present investigation. BK is gratefully acknowledged to the Department of Science and Technology (DST) INSPIRE, New Delhi, for the financial support provided to her in the form of JRF/SRF. Authors also acknowledge the Rajasthan Agricultural Research Institute (RARI), Jaipur, Rajsthan, India, Central Arid Zone Research Institute (CAZRI), Jodhpur and National Bureau of Plant Genetics Resources (NBPGR), Jodhpur, India for providing guar accessions.