Phosphate solubilizing bacteria and arbuscular mycorrhizal fungi associated with Hevea brasiliensis under plantations of Tripura, India

, , ,


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-021-00256-4
First Page: 775
Last Page: 784
Views: 606


Keywords: Hevea brasiliensis , Seasonal, Mycorrhizal colonization, Phosphate solubilization index, Bacterial population


Abstract


The current investigation was focused to enumerate seasonal population of phosphate solubilizing bacteria (PSB) and to examine colonization of arbuscular mycorrhizal (AM) fungi associated with rubber trees from the plantations of Tripura, India. The study also aims to identify AM fungi and characterize PSB from the rhizosphere of rubber trees. The results indicated that AM fungal colonization and PSB population were higher during monsoon and significantly lower in winter. Significant correlation was observed between populations of PSB and AM fungal colonization. Phosphate solubilization index of PSB isolates was significantly exhibited highest by Bacillus tequilensis. Twenty seven AM morphotypes and five isolates of PSB were identified. The higher number of species recovered from the genus having the order Glomus > Rhizophagus > Claroideoglomus =Sclerocystis > Acaulospora = Funneliformis > Ambispora = Diversispora = Dentiscutata = Gigaspora = Redekera = Septoglomus. The results indicated that the rubber plantations harbour robust composition of both the soil microflora.



                Hevea brasiliensis
              , Seasonal, Mycorrhizal colonization, Phosphate solubilization index, Bacterial population


*Pdf Download

(*Only SPR Members can download pdf file; #Open Access;)

References


Alagawadi AR, Gaur AC (1992) Inoculation of Azospirillum brasilense and phosphate solubilizing bacteria on yield of sorghum (Sorghum bicolor L. Moench) in dry land. Trop Agric 69:347–350


Allen SE, Grimshaw MH, Parkinson JA, Quarmby C (1974) Chemical analysis of ecological materials. Blackwell’s Scientific Publications, Oxford


Anderson JM, Ingram JSI (eds) (1993) Tropical soil biology and fertility: a handbook of methods. CAB International, Wallingford


Arcand MM, Schneider KD (2006) Plant- and microbial-based mechanisms to improve the agronomic effectiveness of phosphate rock: a review. An Acad Bras Cienc 78:791–807


Bhattacharya S, Bagyaraj DJ (2002) Arbuscular mycorrhizal fungi associated with Coffea arabica. Geobios 29:93–96


Brundrett M (1991) Mycorrhizas in natural ecosystems. Adv Ecol Res 21:171–313


Cappuccino JG, Sherman N (1999) Microbiology: a laboratory manual, 4th edn. Addison Wesley Longman, Inc., Harlow


Chakraborty K, Sinha S, Debnath A, Roy Das A, Saha AK, Das P (2016) Arbuscular mycorrhizal fungal colonization in three different age group of rubber plantations in Tripura, Northeast India. Plant Pathol Quar 6(2):122–131


Chakraborty K, Talapatra K, Das Roy A, Saha AK, Das P (2019a) Mycorrhizal colonization in Drosera burmannii Vahl, a carnivorous plant: observation using light and fluorescence microscopy. Ambient Sci. https://doi.org/10.21276/ambi.2019.06.1.ra02


Chakraborty K, Banik S, Debnath A, Das Roy A, Saha AK, Das P (2019b) Arbuscular mycorrhiza and dark septate endophyte fungal associations of Oryza sativa L. under field condition: colonization features and their occurrence. Plant Sci Today 6(1):63–70


Chen YP, Rekha PD, Arun AB, Shen FT, Lai WA, Young CC (2006) Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Appl Sci Ecol 34(1):33–41


Cheng C, Wang R, Jiang J (2007) Variation of soil fertility and carbon sequestration by planting Hevea brasilience in Hainan Island, China. J Environ Sci 19(3):348–352


Christopher K, Bruno E (2003) Identification of bacterial species. Tested Stud Lab Teach 24:103–130


Chung H, Park M, Madhaiyan M, Seshadri S, Song J, Cho H, Sa T (2005) Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of crop plants of Korea. Soil Boil Biochem 37:1970–1974


Chun-Qiao X, Ru-an C, Huan H, Wen-xue Z (2009) Characterization of tricalcium phosphate solubilization by Stenotrophomonas maltophilia YC isolated from phosphate mines. J Cent South Univ Technol 16:0581–0587


Das P, Kayang H (2008) Stamp pad ink, an effective stain for observing arbuscular mycorrhizal structure in roots. World J Agric Sci 4:58–60


Deka HK, Philip V, Vinod KK, Krishnakumar AK (1998) Spatial distribution of soil microflora in a five year old rubber plantation in Tripura. Indian J Nat Rubber Res 11(1 & 2):88–93


Dhritiman C, Sharma GD, Jha DK (2014) Isolation and identification of some arbuscular mycorrhiza (AM) fungi for phytoremediation in soil contaminated in paper mill effluent. Intern J Curr Microbiol Appl Sci 3:527–539


Edi-Premono M, Moawad MA, Vleck PLG (1996) Effect of phosphate solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indones J Crop Sci 11:13–23


Fitter AH (2005) Darkness visible: reflections on underground ecology. J Ecol 93:231–243


Fox J, Castella JC (2013) Expansion of rubber (Hevea brasiliensis) in mainland Southeast Asia: what are the prospects for smallholders? J Peasant Stud 40:155–170


Gange AC, Brown VK, Farmer LM (1990) A test of mycorrhizal benefit in an early successional plant community. New Phytol 115:85–91


Gerdemann JW, Nicolson TW (1963) Spores of mycorrhizal endogone species extracted from soil by wet-sieving and decanting method. Trans Br Mycol Soc 46:235–245


Giovannetti M (1985) Seasonal variations of vesicular arbuscular mycorrhizas and endogonaceous spores in a maritime and sand dune. Trans Br Mycol Soc 84:679–684


Gulati A, Sharma N, Vyas P, Sood S, Rahi P, Pathania V, Prasad R (2010) Organic acid production and plant growth promotion as a function of phosphate solubilization by Acinetobacter rhizosphaerae strain BIHB 723 isolated from the cold deserts of the trans-Himalayas. Arch Microbiol 192:975–983


Guoju X, Qiang Z, Jiangtao B, Fengju Z, Chengke L (2012) The relationship between winter temperature rise and soil. Air Soil Water Res 5:15–22


Gyaneshwar PG, Naresh Kumar G, Parekh LJ, Poole PS (2002) Role of soil microorganisms in improving P nutrition of plants. Plant Soil 245:83–93


Harley JL, Smith SE (1983) Mycorrhizal symbiosis. Academic Press, London


Herrmann L, Lesueur D, Bräu L, Davison J, Jairus T, Robain H, Robin A, Vasar M, Wiriyakitnateekul W, Öpik M (2016) Diversity of root-associated arbuscular mycorrhizal fungal communities in a rubber tree plantation chronosequence in Northeast Thailand. Mycorrhiza 26(8):863–877


Jackson ML (1967) Soil chemical analysis. Prentice Hall, New Delhi


Joshi KC, Singh HP (1995) Inter relationships among vesicular arbuscular mycorrhizal population, soil properties and root colonization capacity of soil. J Indian Soc Soil Sci 43:204–207


Koske RE, Tessier B (1983) A convenient, permanent slide mounting medium. Mycol Soc Am News Lett 34:59


Kox HL (2000) Ecological advantages of NR latex production. Nat Rubber 17(1):4


Krishnakumar AK, Meenattoor RJ (2003) Restoration of denuded lands in the north eastern India through rubber plantations. Leisa India, pp 23–24


McGonigle TP, Miller MH, Evans DG, Fairchild GL, Swan JA (1990) A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytol 115:495–501


Minaxi J, Saxena S, Chandra NL (2013) Synergistic effect of phosphate solubilizing rhizobacteria and arbuscular mycorrhiza on growth and yield of wheat plants. J Soil Sci Plant Nutr 13(2):511–525


Mosse B (1973) Advances in the study of vesicular-arbuscular mycorrhiza. Annu Rev Phytopathol 11:171–196


Mukherjee PK, Rai RK (2000) Effect of vesicular arbuscular mycorrhizae and phosphate-solubilizing bacteria on growth, yield and phosphorus uptake by wheat (Triticum aestivum) and chickpea (Cicer arietinum). Indian J Agron 45:602–607


Muthukumar T, Udaiyan K (1999) Spore in spore syndrome in vesicular arbuscular mycorrhizal fungi and its seasonality in a tropical grass land. Nova Hedwigia 68(3–4):339–349


Narula N, Kumar V, Behl RK, Duebel AA, Gransee A, Merbach W (2000) Effect of P solubilizing Azotobacter chroococcum on N, P, K uptake in P responsive wheat genotypes grown under greenhouse conditions. J Plant Nutr Soil Sci 163:393–398


Nautiyal CS (1999) An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol Lett 170:65–270


Pérez E, Sulbarán M, Ball MM, Yarzábal LA (2007) Isolation and characterization of mineral phosphate-solubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region. Soil Biol Biochem 39:2905–2914


Prasanthi S, Jalander V, Mamatha M (2016) The vesicular arbuscular mycorrhizal associations with two medicinal plant species in Telangana University Campus, Nizamabad, T.S. India. Intern J Curr Res Bio Plant Biol 3:62–65


Rajeshkumar PP, Thomas GV, Gupta A, Gopal M (2015) Diversity, richness and degree of colonization of arbuscular mycorrhizal fungi in coconut cultivated along with intercrops in high productive zone of Kerala, India. Symbiosis 65:125–141


Robinson-Boyer L, Grzyb I, Jeferies P (2009) Shifting the balance from qualitative to quantitative analysis of arbuscular mycorrhizal communities in field soils. Fungal Ecol 2:1–9


Rodriguez H, Fraga R (1999) Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17:319–339


Saeid A, Prochownik E, Dobrowolska-Iwanek J (2018) Phosphorus solubilization by Bacillus species. Molecules 23(11):2897. https://doi.org/10.3390/molecules23112897


Selvaraj P, Madhaiyan M, Sa T (2008) Isolation and identification of phosphate solubilizing bacteria from Chinese cabbage and their effect on growth and phosphorus utilization of plants. J Microbiol Biotechnol 18:773–777


Sindhu OP, Behl HM (1997) Response of three Glomus species on growth of Prosopis juliflora Swartz at high pH levels. Symbiosis 23:23–24


Singh S, Kapoor K (1998) Effects of inoculation of phosphate-solubilizing microorganisms and an arbuscular mycorrhizal fungus on mungbean grown under natural soil conditions. Mycorrhiza 7:249–253


Singh S, Kapoor KK (1999) Inoculation with phosphate-solubilizing microorganisms and a vesicular–arbuscular mycorrhizal fungus improves dry matter yield and nutrient uptake by wheat grown in a sandy soil. Biol Fertil Soils 28:139–144


Singh H, Kaur M, Sharma S, Kaur L, Mishra S, Tanuku NRS, Pinnaka AK (2018) Bacillus alkalilacus sp. nov., isolated from a sediment sample from a lake in India. Int J Syst Evol Microbiol 68:1665–1671


Smith SE, Read DJ (2008) Mycorrhizal symbiosis, 3rd edn. Academic Press, London


Štajerová K, Šmilauerová M, Šmilauer P (2009) Arbuscular mycorrhizal symbiosis of herbaceous invasive neophytes in the Czech Republic. Preslia 81:341–355


Summuna B, Sheikh PA, Gupta S, Dar WA, Dar GH (2019) Diversity and distribution of arbuscular mycorrhizal fungi in pome and stone fruit orchards of North Western Himalayan State of Jammu & Kashmir. Indian Phytopathol. https://doi.org/10.1007/s42360-019-00120-11


Tedersoo L, Sánchez-Ramírez S, Kõljalg U, Bahram M, Döring M, Schigel D, May T, Ryberg M, Abarenkov K (2018) High-level classification of the fungi and a tool for evolutionary ecological analyses. Fungal Divers. https://doi.org/10.1007/s13225-018-0401-0(0123456789()

 


Acknowledgements


The authors are thankful to Head, Department of Botany, Tripura University for providing the laboratory facilities. KC is grateful to Department of Science and Technology, Government of India for the INSPIRE fellowship. The authors are thankful to Institute of Microbial Technology (IMTECH), Chandigarh, India for molecular identification of the bacterial isolates.


Author Information


Chakraborty Kripamoy
Microbiology Laboratory, Department of Botany, Tripura University, Agartala, India
kripachakraborty@gmail.com
Das Aparajita Roy
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Agartala, India


Saha Ajay Krishna
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Agartala, India


Das Panna
Microbiology Laboratory, Department of Botany, Tripura University, Agartala, India