Photosynthetic efficiency in Gymnema sylvestre (Retz.) R.Br. genotypes

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-019-00062-z
First Page: 473
Last Page: 485
Views: 1871


Keywords: Chlorophyll fluorescence, Gymnema sylvestre , Maximum efficiency of photosynthesis, Stomatal conductance, Water use efficiency


Abstract

Leaves of Gymnema sylvestre (Retz.) R.Br. ex SCHULT plants are used to cure the most prevalent and important life style disease, the diabetes mellitus. Gymnema is a slow growing plant genotype with the requirement of higher photosynthetic rate. Presently, there is no report available on gaseous exchange and chlorophyll fluorescence parameters in gymnema. Total 44 genotypes were evaluated for their physiological efficiencies during post rainy season in sub-tropical region of Gujarat.The light curve studies indicated the light requirement of the plants to be between 1000 and 1250 µ mol (photons) m−2 s−1. Genotypes were categorized into low, medium and high groups considering over all mean values of different parameters ± standard deviation. Seven genotypes showed higher photosynthetic rate (PN), nine genotypes showed higher stomatal conductance (gs), six genotype exhibited higher transpiration rate (E), five genotypes revealed higher intrinsic water use efficiency (WUEint), eight genotypes manifested maximum photochemical efficiency (Fv/Fm), seven genotypes reached maximum actual efficiency of PSII (({ ext{F}}_{ ext{v}}^{{prime }})/({ ext{F}}_{ ext{m}}^{{prime }})), six genotype showed maximum quantum yield of PSII (ɸPSII) and electron transport rate (ETR) and seven genotypes exhibited higher open PS II centers (qP). The correlation matrix revealed that the PN was strongly positively correlated with gs, ({ ext{F}}_{ ext{v}}^{{prime }})/({ ext{F}}_{ ext{m}}^{{prime }}), ΦPSII and ETR. DGS-2 and DGS-18 were physiologically most efficient genotypes having high values in seven common parameters. These parameters were PN, gs, Fv/Fm, ({ ext{F}}_{ ext{v}}^{{prime }})/({ ext{F}}_{ ext{m}}^{{prime }}), ΦPSII, qP and ETR will be very useful in crop improvement program focused on biomass maximization in Gymnema sylvestre.


Chlorophyll fluorescence, Gymnema sylvestre , Maximum efficiency of photosynthesis, Stomatal conductance, Water use efficiency


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References

  1. Ahmed A, Rao AS, Rao MV, Taha RM (2012) Production of Gymnemic acid depends on medium, explants, PGRs, color lights, temperature, photoperiod, and sucrose sources in batch culture of Gymnema sylvestre. Sci World J 1–11

  2. Ainsworth EA, Long SP (2005) What have we learned from 15 years of free air CO2 enrichment (FACE) A metaanalytic review of the responses of photosynthesis, canopy properties and plant production to rising CO2. New Phytol 165(2):351–372

  3. Araus JL, Amaro T, Voltas J, Nakkoul H, Nachit MM (1998) Chlorophyll fluorescence as a selection criterion for grain yield in durum wheat under Mediterranean conditions. Field Crops Res 55(3):209–223

  4. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24(1):1

  5. Baker NR, Horton P (1987) Physiological factors associated with fluorescence quenching during photoinhibition. In: Arntzen CJ, Kyle DJ, Osmond CB (eds) Topics in photosynthesis, photoinhibition. Elsevier, The Netherlands, pp 9145–9168

  6. Belko N, Zaman-Allah M, Cisse N, Diop NN, Zombre G, Ehlers JD, Vadez V (2012) Lower soil moisture threshold for transpiration decline under water deficit correlates with lower canopy conductance and higher transpiration efficiency in drought-tolerant cowpea. Funct Plant Biol 39(4):306–322

  7. Bilger W, Björkman O (1990) Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hederacanariensis. Photosynth Res 25(3):173–185

  8. Björkman O, Demmig-Adams B (1995) Regulation of photosynthetic light energy capture, conversion, and dissipation in leaves of higher plants. Ecophysiology of photosynthesis. Springer, Berlin, pp 17–47

  9. Bogale A, Tesfaye K, Geleto T (2011) Morphological and physiological attributes associated to drought tolerance of Ethiopian durum wheat genotypes under water deficit condition. J Biodivers Environ Sci 1(2):22–36

  10. Butler WL, Kitajima M (1975) Fluorescence quenching in photosystem II of chloroplasts. Biochimica et BiophysicaActa (BBA)-Bioenergetics 376(1):116–125

  11. Cao T, Isoda A (2008) Dry matter production of Japanese and Chinese high-yielding peanut cultivars under dense planting in terms of intercepted radiation and its use efficiency. Jpn J Crop Sci 77(1):41

  12. Dhanani T, Singh R, Waman A, Patel P, Manivel P, Kumar S (2015) Assessment of diversity amongst natural populations of Gymnema sylvestre from India and development of a validated HPLC protocol for identification and quantification of gymnemagenin. Ind Crops Prod 77:901–909

  13. Dwyer JF, McPherson EG, Schroeder HW, Rowntree RA (1992) Assessing the benefits and costs of the urban forest. J Arboricult 18:227

  14. Finazzi G, Johnson GN, Dall’Osto L, Zito F, Bonente G, Bassi R, Wollman FA (2006) Nonphotochemical quenching of chlorophyll fluorescence in Chlamydomonas reinhardtii. Biochem 45(5):1490–1498

  15. Fracheboud Y, Jompuk C, Ribaut JM, Stamp P, Leipner J (2004) Genetic analysis of cold-tolerance of photosynthesis in maize. Plant Mol Biol 56(2):241–253

  16. Genty B, Briantais JM, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et BiophysicaActa (BBA)-General Subjects 990(1):87–92

  17. Guo P, Li R (2000) Effects of high nocturnal temperature on photosynthetic organization in rice leaves. Acta BotanicaSinica 42(7):673–678

  18. Isoda A (2010) Effects of water stress on leaf temperature and chlorophyll fluorescence parameters in cotton and peanut. Plant Prod Sci 13(3):269–278

  19. Kalariya KA, Singh AL, Chakraborty K, Zala PV, Patel CB (2013) Photosynthetic characteristics of groundnut (Arachishypogaea L.) under water deficit stress. Indian J Plant Physiol 18(2):157–163

  20. Kalariya KA, Singh AL, Goswami N, Mehta D, Mahatma MK, Ajay BC, Chakraborty K, Zala PV, Chaudhary V, Patel CB (2015) Photosynthetic characteristics of peanut genotypes under excess and deficit irrigation during summer. Physiol Mol Biol Plants 21(3):317–327

  21. Kalariya KA, Goshwami Nisha, Mehta Deepti, Singh AL, Saran PL (2019) Chlorophyll fluorescence: a physiological mechanism and a physical tool in plant eco-physiological studies. IN Adv Plant Physiol 18:201–242

  22. Krause GH, Weis E (1991) Chlorophyll fluorescence and photosynthesis: the basics. Annu Rev Plant Biol 42(1):313–349

  23. Krishna RB, Reddy SR, Vijayalakshmi M, Anugna K, Divyavani N, Reddy KJ (2012) Molecular characterization of 17 accessions of Gymnema sylvestre R. Br. using RAPD markers. Int J Pharma Biosci 3:126–135

  24. Liu G, Yang C, Xu K, Zhang Z, Li D, Wu Z, Chen Z (2012) Development of yield and some photosynthetic characteristics during 82 years of genetic improvement of soybean genotypes in northeast China. Aust J Crop Sci 6(10):1416

  25. Maheshwari N, Nadgauda RS (2016) Variation in gymnemic acid content in Gymnema germplasm from different geographical regions of Gujarat. J Chem Pharma Res 8(3):37–41

  26. Maxwell K, Johnson GN (2000) Chlorophyll fluorescence—a practical guide. J Exp Bot 51(345):659–668

  27. Misra AN (1993) Molecular mechanism of turn-over of 32 kDa-herbicide binding protein of photosyntem II reaction center. Adv Plant Biotechnol Biochem 73–78

  28. Misra AN, Terashima I (2003) Changes in photosystem activities during adapatation of Viciafaba seedlings to low, moderate and high temperatures. Plant Cell Physiol

  29. Misra AN, Ramaswamy NK, Desai TS (1997) Thermoluminescence studies on the photoinhibition of pothos leaf discs at chilling, room and high temperature. J Photochem Photobiol B: Biol 38(2–3):164–168

  30. Misra AN, Srivastava A, Strasser RJ (2001a) Utilization of fast chlorophyll a fluorescence technique in assessing the salt/ion sensitivity of mung bean and Brassica seedlings. J Plant Physiol 158(9):1173–1181

  31. Misra AN, Srivastava A, Strasser RJ (2001-b) Fast chlorophyll a fluorescence kinetic analysis for the assessment of temperature and light effects: A dynamic model for stress recovery phenomena, Photosynthsis, PS2001 CSIRO Publ., Melbourne, Australia S3-007

  32. Misra AN, Srivastava A, Strasser RJ (2007) Elastic and plastic responses of Viciafaba leaves to high temperature and high light stress. In: Gordon Conference on “Temperature stress in plants”

  33. Müller P, Li XP, Niyogi KK (2001) Non-photochemical quenching. A response to excess light energy. Plant physiology 125(4):1558–1566

  34. Nigam SN, Chandra S, Sridevi KR, Bhukta M, Reddy AG, Rachaputi NR, Wright GC, Reddy PV, Deshmukh MP, Mathur RK, Basu MS (2005) Efficiency of physiological trait-based and empirical selection approaches for drought tolerance in groundnut. Ann Appl Biol 146(4):433–439

  35. Onofri A (2007) Routine statistical analyses of field experiments by using an Excel extension. In: 6th National Conference of the Italian Biometric Society 20 (Vol. 2022)

  36. Rahbarian R, Khavari-Nejad R, Ganjeali A, Bagheri A, Najafi F (2011) Drought stress effects on photosynthesis, chlorophyll fluorescence and water relations in tolerant and susceptible chickpea (Cicerarietinum L.) genotypes. ActaBiologicaCracoviensia Series Botanica 53(1):47–56

  37. Ravindra V, Rathnakumar AL, Ajay BC, Zala PV (2012) Genetic variations in photosynthetic rate, pod yield and yield components in Spanish groundnut cultivars during three cropping seasons. Field Crops Res 18(125):83–91

  38. Richards RA (2000) Selectable traits to increase crop photosynthesis and yield of grain crops. J Exp Bot 51(suppl_1):447–458

  39. Roháček K, Soukupová J, Barták M (2008) Chlorophyll fluorescence: a wonderful tool to study plant physiology and plant stress (2012) Plant Cell Compartments-Selected Topics. Research Signpost, Kerala, p 41-10

  40. Schreiber U (2004) Pulse-amplitude-modulation (PAM) fluorometry and saturation pulse method: an overview. Chlorophylla fluorescence. Springer, Dordrecht, pp 279–319

  41. Schreiber U, Schliwa U, Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10(1–2):51–62

  42. Shahnawaz M, Zanan RL, Wakte KV, Mathure SV, Kad TD, Deokule SS, Nadaf AB (2012) Genetic diversity assessment of Gymnema sylvestre (Retz.) R. Br. ex Sm. populations from Western Ghats of Maharashtra, India. Genetic Resour Crop Evolut 59(1):125–134

  43. Sinclair TR (2012) Is transpiration efficiency a viable plant trait in breeding for crop improvement. Funct Plant Biol 39(5):359–365

  44. Singh AL, Nakar RN, Chakraborty K, Kalariya KA (2014) Physiological efficiencies in mini-core peanut germplasm accessions during summer season. Photosynthetica 52(4):627–635

  45. Songsri P, Jogloy S, Holbrook CC, Kesmala T, Vorasoot N, Akkasaeng C, Patanothai A (2009) Association of root, specific leaf area and SPAD chlorophyll meter reading to water use efficiency of peanut under different available soil water. Agric Water Manag 96(5):790–798

  46. Zimare SB, Borde MY, Jite PK, Malpathak NP (2013) Effect of AM Fungi (Gf, Gm) on Biomass and Gymnemic Acid Content of Gymnema sylvestre (Retz.) R. Br. ex Sm. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 83(3):439-45


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Acknowledgements

Authors are grateful to the Indian Council of Agricultural Research, New Delhi and the Director, ICAR-DMAPR, Anand for providing all basic facilities and the Gujarat State Biotechnology Mission, Government of Gujarat, India for funding assistance. We are also thankful to the germplasm collectors and curators of the germplasm used in this study.


Author Information

Kalariya Kuldeepsingh A.
ICAR-Directorate of Medicinal and Aromatic Plants Research, Anand, India
Kuldeep_ka@yahoo.co.in
Minipara Dipal
ICAR-Directorate of Medicinal and Aromatic Plants Research, Anand, India


Saran Parmeshwar Lal
ICAR-Directorate of Medicinal and Aromatic Plants Research, Anand, India


Poojara Lipi
ICAR-Directorate of Medicinal and Aromatic Plants Research, Anand, India


Polireddy A. C.
ICAR-Directorate of Medicinal and Aromatic Plants Research, Anand, India

Manivel P.
ICAR-Directorate of Medicinal and Aromatic Plants Research, Anand, India