Vegetos
(An International Journal of Plant Research & Biotechnology)
Phytochemical analysis and antioxidant activity of Marchantia paleacea Bertol.
*Article not assigned to an issue yet
Dogra Neha, Secrain Swati, Pannu Anshul, Sabharwal Hiteshi, Kapila Sunita
Research Articles | Published: 14 April, 2025
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Keywords: n Marchantia paleacean , Bioactive compounds, Bisbibenzyls, Palmitic acid β-monoglyceride, GC-MS, LC-MS
Abstract
Bryophytes are the oldest known plants and have been utilised in traditional Chinese medicine since antiquity. The present study aimed to investigate the phytochemical composition and antioxidant activity of liverwort Marchantia paleacea. Soxhlet apparatus was used to prepare different solvent (water, methanol, acetone, petroleum ether and hexane) extracts of M. paleacea. The quantification of total phenols, flavonoids, terpenoids and tannins was carried out by implementing spectrophotometric techniques. The antioxidant activity of M. paleacea was determined using DPPH, ABTS+, metal chelating, total antioxidant capacity and FRAP assays. Furthermore, the functional groups and bioactive profile of the liverwort were studied employing FTIR, GC-MS and LC-MS techniques. The total phenols (141.5 mg GAE/g), flavonoids (118.31 mg RE/g), terpenoids (31.61 mg LE/g) and tannins (97.45 mg TAE/g) were significantly higher in methanol than other solvent fractions. Also, methanol extract exhibited the highest DPPH inhibition (99.7μg/ml), metal chelation (179.8μg/ml) and reducing potential (108.92 μM FeSO4 E/g). The maximum ABTS+ scavenging (67.7μg/ml) and total antioxidant capacity (83.09 μg AAE/mg) were reported in acetone extract. The MS spectra of the methanol extract of M. paleacea showed the predominance of compounds: marchantins, palmitic acid β-monoglyceride, phytol and stigmasterol. Results indicate that the significant antioxidant activity of M. paleacea might be attributed to the polyphenols and bisbibenzyls.
References
Abarca-Vargas R, Pena Malacara CF, Petricevich VL (2016) Characterization of chemical compounds with antioxidant and cytotoxic activities in Bougainvillea x buttiana holttum and standl,(Var. rose) extracts. Antioxid 5(4):45. https://doi.org/10.3390/antiox5040045
Abu Bakar MF, Abdul Karim F, Suleiman M, Isha A, Rahmat A (2015) Phytochemical constituents, antioxidant and antiproliferative properties of a liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah Malaysia. Evid Based Complement Altern Med 2015:936215. https://doi.org/10.1155/2015/936215
Alam MN, Bristi NJ, Rafiquzzaman M (2013) Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J 21(2):143–152. https://doi.org/10.1016/j.jsps.2012.05.002
Alothman M, Bhat R, Karim AA (2009) Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food Chem 115(3):785–788. https://doi.org/10.1016/j.foodchem.2008.12.005
Al-Shabibi MHS, Al-Touby SSJ, Hossain MA (2022) Isolation, characterization and prediction of biologically active glycoside compounds quercetin-3-rutinoside from the fruits of Ficus sycomorus. Carbohyd Res 511:108483. https://doi.org/10.1016/j.carres.2021.108483
Amalraj S, Mariyammal V, Murugan R, Gurav SS, Krupa J, Ayyanar M (2021) Comparative evaluation on chemical composition, in vitro antioxidant, antidiabetic and antibacterial activities of various solvent extracts of Dregea volubilis leaves. S Afr J Bot 138:115–123. https://doi.org/10.1016/j.sajb.2020.12.013
Asakawa Y (1982) Chemical constituents of the Hepaticae. Springer, Vienna
Asakawa Y (2001) Recent advances in phytochemistry of bryophytes-acetogenins, terpenoids and bis(bibenzyl)s from selected Japanese, Taiwanese, New Zealand Argentinean and European liverworts. Phytochemistry 56(3):297–312. https://doi.org/10.1016/S0031-9422(00)00454-4
Asakawa Y (2017) The isolation, structure elucidation, and bio-and total synthesis of bis-bibenzyls, from liverworts and their biological activity. Nat Prod Commun 12(8):1335–1349. https://doi.org/10.1177/1934578X1701200845
Bakrim S, Benkhaira N, Bourais I, Benali T, Lee LH, El Omari N, Sheikh RA, Goh KW, Ming LC, Bouyahya A (2022) Health benefits and pharmacological properties of stigmasterol. Antioxidants 11(10):1912. https://doi.org/10.3390/antiox11101912
Banakar P, Jayaraj M (2018) GC-MS analysis of bioactive compounds from ethanolic leaf extract of Waltheria indica Linn and their pharmacological activities. Int J Pharm Sci Res 9(5):2005–2010. https://doi.org/10.13040/IJPSR.0975-8232.9(5).2005-10
Boeing JS, Barizão ÉO, e Silva BC, Montanher PF, de CinqueAlmeida V, Visentainer JV (2014) Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis. Chem Cent J 8:1–9
Cai YY, Chen T, Cao JF (2022) Antimicrobial and antioxidant metabolites from the cultured suspension cells of Marchantia polymorpha L. Nat Prod Commun 17(4):1–7. https://doi.org/10.1177/1934578X221096172
Cao H, Xiao JB, Xu M (2007) Comparison of volatile components of Marchantia convoluta obtained by supercritical carbon dioxide extraction and petrol ether extraction. J Food Compos Anal 20(1):45–51. https://doi.org/10.1016/j.jfca.2006.06.004
Carvalho AMS, Heimfarth L, Pereira EWM, Oliveira FS, Menezes IRA, Coutinho HDM, Picot L, Antoniolli AR, Quintans JSS, Quintans-Júnior LJ (2020) Phytol, a chlorophyll component, produces antihyperalgesic, anti-inflammatory, and antiarthritic effects: possible NFκB pathway involvement and reduced levels of the proinflammatory cytokines TNF-α and IL-6. J Nat Prod 83(4):1107–1117. https://doi.org/10.1021/acs.jnatprod.9b01116
Chan JYW, Tang PMK, Hon PM, Au SWN, Tsui SKW, Waye MMY, Kong SK, Mak TCW, Fung KP (2006) Pheophorbide a, a major antitumor component purified from Scutellaria barbata, induces apoptosis in human hepatocellular carcinoma cells. Planta Med 72(01):28–33
Chen WP, Yu C, Hu PF, Bao JP, Tang JL, Wu LD (2012) Stigmasterol blocks cartilage degradation in rabbit model of osteoarthritis. Acta Biochim Pol 59(4):537–541
Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, Ju YH (2014) Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J Food Drug Anal 22(3):296–302. https://doi.org/10.1016/j.jfda.2013.11.001
Dogra N, Kapila S, Secrain S, Pannu A, Sethi M, Sharma S, Sabharwal H (2024) Phytochemical analysis and antioxidant activity of an Indian liverwort Dumortiera hirsuta subsp. Hirsuta (Sw.) Nees. Plant Arch 24(1):83–92. https://doi.org/10.51470/PLANTARCHIVES.2024.v24.no.1.013
Ehiobu JM, Idamokoro ME, Afolayan AJ (2021) Phytochemical content and antioxidant potential of leaf extracts of Citrus limon (L.) Osbeck collected in the Eastern Cape Province South Africa. S Afr J Bot 141:480–486. https://doi.org/10.1016/j.sajb.2021.06.001
Ghorai N, Chakraborty S, Gucchait S, Saha SK, Biswas S (2012) Estimation of total terpenoids concentration in plant tissues using a monoterpene Linalool as Standard reagent. Protoc Exch 5(10):1–5. https://doi.org/10.1038/protex.2012.055
Gökbulut A, Satilmiş B, Batçioğlu K, Çetın B, Şarer E (2012) Antioxidant activity and luteolin content of Marchantia polymorpha L. Turk J Biol 36(4):381–385. https://doi.org/10.3906/biy-1106-15
Gutiérrez-del-Río I, López-Ibáñez S, Magadán-Corpas P, Fernández-Calleja L, Pérez-Valero Á, Tuñón-Granda M, Miguélez EM, Villar CJ, Lombó F (2021) Terpenoids and polyphenols as natural antioxidant agents in food preservation. Antioxid 10(8):1264. https://doi.org/10.3390/antiox10081264
Huang WJ, Wu CL, Lin CW, Chi LL, Chen PY, Chiu CJ, Huang CY, Chen CN (2010) Marchantin A, a cyclic bis (bibenzyl ether), isolated from the liverwort Marchantia emarginata subsp Tosana induces apoptosis in human MCF-7 breast cancer cells. Cancer Lett 291(1):108–119. https://doi.org/10.1016/j.canlet.2009.10.006
Iqbal E, Salim KA, Lim LB (2015) Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus (Airy Shaw) from Brunei Darussalam. J King Saud Uni Sci 27(3):224–232. https://doi.org/10.1016/j.jksus.2015.02.003
Iwai Y, Murakami K, Gomi Y, Hashimoto T, Asakawa Y, Okuno Y, Ishikawa T, Hatakeyama D, Echigo N, Kuzuhara T (2011) Anti-influenza activity of marchantins, macrocyclic bisbibenzyls contained in liverworts. PLoS One 6(5):e19825. https://doi.org/10.1371/journal.pone.0019825
Jensen JSR, Omarsdottir S, Thorsteinsdottir JB, Ogmundsdottir HM, Olafsdottir ES (2012) Synergistic cytotoxic effect of the microtubule inhibitor marchantin A from Marchantia polymorpha and the Aurora kinase inhibitor MlN8237 on breast cancer cells in vitro. Planta Med 78(5):448–454. https://doi.org/10.1055/s-0031-1298230
Ji M, Shi Y, Lou H (2011) Overcoming of P-glycoprotein-mediated multidrug resistance in K562/A02 cells using riccardin F and pakyonol, bisbibenzyl derivatives from liverworts. BioSci Trends 5(5):192–197. https://doi.org/10.5582/bst.2011.v5.5.192
Jie F, Yang X, Yang B, Liu Y, Wu L, Lu B (2022) Stigmasterol attenuates inflammatory response of microglia via NF-κB and NLRP3 signaling by AMPK activation. Biomed Pharmacother 153:113317. https://doi.org/10.1016/j.biopha.2022.113317
John KMM, Ayyanar M, Arumugam T, Enkhtaivan G, Jin K, Kim DH (2015) Phytochemical screening and antioxidant activity of different solvent extracts from Strychnos minor Dennst leaves. Asian Pac J Trop Dis 5(3):204–209. https://doi.org/10.1016/S2222-1808(14)60654-X
Kashyap SR (1929) Liverworts of the western Himalayas and the Panjab Plain I: supplement. Univ Punjab, Lahore. 1:1–129
Kashyap SR, Chopra RN (1932) Liverworts of the Western Himalayas and the Panjab Plain II: Supplement. Univ Punjab, Lahore. 2:1–137
Kavitha CCI, Indira G (2016) Quantitative estimation of total phenolic, flavonoids, tannin and chlorophyll content of leaves of Strobilanthes kunthiana (Neelakurinji). J Med Plants Stud 4:282–286
Kumar PRZA, Bhaskar A (2012) Phytochemical evaluation by GC-MS and in vitro antioxidant activity of Punica granatum fruit rind extract. J Chem Pharm Res 4(6):2869–2873
Luo Y, Shang P, Li D (2017) Luteolin: a flavonoid that has multiple cardio-protective effects and its molecular mechanisms. Front Pharmacol 8:692. https://doi.org/10.3389/fphar.2017.00692
Manoj GS, Murugan K (2012) Phenolic profiles, antimicrobial and antioxidant potentiality of methanolic extract of a liverwort, Plagiochila beddomei Steph. Indian J Nat Prod Resour 3(2):173–183
Mohandas GG, Kumaraswamy M (2018) Antioxidant activities of terpenoids from Thuidium tamariscellum (C. Muell.) Bosch and Sande-Lac. a Moss. Pharmacogn J 10(4):645–49. https://doi.org/10.5530/pj.2018.4.106
Mukherjee S, Dey A, De A, Ghosh P (2012) Antioxidative potential of two Darjeeling Himalayan Marchantia sp.:M. paleacea and M. papillata. Util Manag Med Plants 2:289–96
Mukhia S, Mandal P, Singh DK, Singh D, Choudhury D (2014) In-vitro free-radical scavenging potential of three liverworts of Darjeeling Himalaya. Int J Pharm Sci Res 5(10):4552–61. https://doi.org/10.13040/IJPSR.0975-8232.5(10).4552-61
Naczk M, Shahidi F (2006) Phenolics in cereals, fruits and vegetables: occurrence, extraction and analysis. J Pharm Biomed Anal 41(5):1523–1542. https://doi.org/10.1016/j.jpba.2006.04.002
Nandy S, Dey A (2020) Bibenzyls and bisbybenzyls of bryophytic origin as promising source of novel therapeutics: pharmacology, synthesis and structure-activity. DARU J Pharm Sci 28:701–34. https://doi.org/10.1007/s40199-020-00341-0
Negi K, Asthana AK, Chaturvedi P (2020) GC–MS analysis and antifungal activity of acetone extract of Conocephalum conicum (L) Underw (liverwort) against aflatoxins producing fungi. S Afr J Bot 131:384–390. https://doi.org/10.1016/j.sajb.2020.02.035
Panda S, Jafri M, Kar A, Meheta BK (2009) Thyroid inhibitory, antiperoxidative and hypoglycemic effects of stigmasterol isolated from Butea monosperma. Fitoterapia 80(2):123–126. https://doi.org/10.1016/j.fitote.2008.12.002
Pannu A, Kapila S, Secrain S, Sabharwal H, Sethi M, Sharma S, Dogra N (2024) Phytochemical characterization and antifungal activity of Marchantia polymorpha L. against Rhizoctonia solani. Pharmacol Res-Mod Chin Med 11:100426. https://doi.org/10.1016/j.prmcm.2024.100426
Patle TK, Shrivas K, Kurrey R, Upadhyay S, Jangde R, Chauhan R (2020) Phytochemical screening and determination of phenolics and flavonoids in Dillenia pentagyna using UV–vis and FTIR spectroscopy. Spectrochim Acta A Mol Biomol Spectrosc 242:118717. https://doi.org/10.1016/j.saa.2020.118717
Pratiwi R, Nantasenamat C, Ruankham W, Suwanjang W, Prachayasittikul V, Prachayasittikul S, Phopin K (2021) Mechanisms and neuroprotective activities of stigmasterol against oxidative stress-induced neuronal cell death via sirtuin family. Front Nutr 8:648995. https://doi.org/10.3389/fnut.2021.648995
Rao MRK, Anisha G, Prabhu K, Shil S, Vijayalakshmi N (2019) Preliminary phytochemical and gas chromatography–mass spectrometry study of one medicinal plant Carissa carandas. Drug Invent Today 12(8):1629–1630
Rasheed H, Hussain A, Khan MA, Hassan M, Bokhari SAI (2024) Antioxidant dimension and potential as bacterial growth inhibitors in crude extracts of two Artemisia species (Artemisia indica Willd and Artemisia maritima L) from Gilgit-Baltistan region, Pakistan. Vegetos. https://doi.org/10.1007/s42535-024-01032-w
Sabharwal H, Shukla G, Kondepudi KK, Maurya R, Kapila S, Dogra N (2023) Phytochemical analysis and in vitro assessment of extracts of Rhodobryum roseum for antioxidant, antibacterial and anti-inflammatory activities. J Herbs Spices Med Plants 29(4):419–437. https://doi.org/10.1080/10496475.2023.2211288
Sharma R, Singh S, Mareddy NSR, Merchant N, Alam A (2023) Gas chromatography-mass spectroscopic profiling and cytotoxic activity of Riccia billardieri Mont & nees (Bryophyta: Liverwort). Results Chem 6:101004. https://doi.org/10.1016/j.rechem.2023.101004
Shi YQ, Zhu CJ, Yuan HQ, Li BQ, Gao J, Qu XJ, Sun B, Cheng YN, Li S, Li X, Lou HX (2009) Marchantin C, a novel microtubule inhibitor from liverwort with anti-tumor activity both in vivo and in vitro. Cancer Lett 276(2):160–70. https://doi.org/10.1016/j.canlet.2008.11.004
Singh SK, Singh DK (2009) Hepaticae and anthocerotae of great Himalayan national park and its environs (HP), India. Botanical Survey of India, Dehra Dun
Singh D, Singh DK (2013) An Appraisal of the genus Marchantia in India with a note on Marchantia emarginata subspecies emarginata in Indian Himalayan region. Proc Nati Acad Sci, India Sect B: Biol Sci 83:15–26. https://doi.org/10.1007/s40011-012-0065-6
Siregar ES, Pasaribu N, Sofyan MZ (2021) Antioxidant activity of liverworts Marchantia paleacea Bertol from north Sumatra Indonesia. IOP Conf Ser: Earth Environ Sci 713(1):012061. https://doi.org/10.1088/1755-1315/713/1/012061
Snyder LR (1974) Classification of the solvent properties of common liquids. J Chromatogr 92(2):223–230. https://doi.org/10.1016/S0021-9673(00)85732-5
Tan CY, Inagaki M, Chai HB, Lambrechts MK, Önder A, Kiremit HÖ, Rakotondraibe LH (2017) Phytochemical and cytotoxic investigations of pinguisanoids from liverwort Porella cordaeana. Phytochem Lett 19:77–82. https://doi.org/10.1016/j.phytol.2016.12.003
Tanruean K, Poolprasert P, Suwannarach N, Kumla J, Lumyong S (2021) Phytochemical analysis and evaluation of antioxidant and biological activities of extracts from three clauseneae plants in Northern Thailand. Plants 10(1):117. https://doi.org/10.3390/plants10010117
Tyagi S, Singh RK, Tiwari SP (2021) Anti-enterococcal and anti-oxidative potential of a thermophilic cyanobacterium, Leptolyngbya sp HNBGU 003. Saudi J Bio Sci 28(7):4022–28. https://doi.org/10.1016/j.sjbs.2021.04.003
Ullah H, Khan A, Rehma NU, Halim SA, Khan H, Khan I, Csuk R, Al-Rawahi A, Al-Hatmi S, Al-Harrasi A (2020) Lophenol and lathosterol from resin of Commiphora kua possess hepatoprotective effects in vivo. J Ethnopharmacol 252:112558. https://doi.org/10.1016/j.jep.2020.112558
Wang J, Huang M, Yang J, Ma X, Zheng S, Deng S, Huang Y, Yang X, Zhao P (2017) Anti-diabetic activity of stigmasterol from soybean oil by targeting the GLUT4 glucose transporter. Food Nutr Res 61:1364117. https://doi.org/10.1080/16546628.2017.1364117
Xie CF, Qu JB, Wu XZ, Liu N, Ji M, Lou HX (2010) Antifungal macrocyclic bis (bibenzyls) from the Chinese liverwort Plagiochasma intermedium L. Nat Prod Res 24(6):515–20. https://doi.org/10.1080/14786410802271587
Author Information
Department of Botany, Panjab University, Chandigarh, India