Vegetos
(An International Journal of Plant Research & Biotechnology)
Phytochemical composition, antioxidant and antibacterial properties of methanol stem and leaf extracts of Croton bonplandianus Baill
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Research Articles | Published: 15 October, 2024
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Keywords: n Croton bonplandianusn , Total phenolics, Natural antioxidants, In-vitro assay, Heavy metals
Abstract
The increasing demand for natural antioxidants in the food and pharmaceutical sectors has driven the search for plant species with notable bioactive properties. To fulfil the demand for natural antioxidants, different plants including Croton spp. have been explored. However, the antioxidant activities of Croton bonplandianus Baill plant growing in Varanasi and surrounding regions have not been assessed to date. C. bonplandianus thrives in diverse habitats and exhibits a range of bioactivities, such as wound healing, anti-arthritic, antimicrobial, anticancer, and other therapeutic potentials. Therefore, the present study aimed to quantify total phenolic and flavonoid contents in methanol extracts of the leaf (LME) and stem (SME) of C. bonplandianus plant. Further, extracts were analyzed for in-vitro antioxidant and antibacterial activities. The results depicted that total phenolics (mg GAE/g dw) and flavonoids (mg QE/g dw) content were higher in LME (63.2 and 4.3, respectively) of C. bonplandianus as compared to the SME (30.5 and 3.5, respectively). Further, the results revealed DPPH (%), ABTS (%) and FRAP [µM Fe (II)/g dw] activities in LME (78.7, 83.4 and 203.8, respectively) were also higher than SME (72.3, 71.73 and 169.7, respectively). IC50 values were recorded as 0.8 mg/ml and 5.8 mg/ml for LME and SME, respectively. GC–MS investigation of LME and SME showed the presence of Undecane 2,6-dmethyl (28%) followed by cyclonasilaxane (19%), octadecanoic acid (6.62%) and hexadecanoic acid (4.18%) as major compounds. Both the extract potentially possessed antibacterial properties against E. coli. The findings also demonstrated that heavy metal concentration in soil, leaf, and stem was found within Indian norms. The present study concludes that both, the leaf and stem of C. bonplandianus plant are rich in phenolic and possess antioxidant and antibacterial activities. Therefore, it is suggested that C. bonplandianus be further explored and used therapeutically to fulfill the current demand for natural antioxidants.
References
Ali NM, Annegowda HV, Mansor SM, Ismail S, Ramanathan S, Mordi MN (2012) Phytochemical screening, antioxidant and analgesic activities of Croton argyratus ethanolic extracts. J Med Plants Res 6(21):3724–3731
Allen SE, Grimshaw HM, Rowland AP (1986) Chemical analysis. In: Moore PD, Chapman SB (eds) Methods in plant ecology. Blackwell Scientific Publication, Oxford, pp 285–344
Ambigaipalan P, de Camargo AC, Shahidi F (2016) Phenolic compounds of pomegranate byproducts (outer skin, mesocarp, divider membrane) and their antioxidant activities. J Agric Food Chem 64(34):6584–6604
Awashthi SK (2000) Central and state rules as amended for 1999: prevention of food adulteration act no 37 of 1954. Ashoka Law House, New Delhi
Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239(1):70–76
Bernal-Mercado AT, Gutierrez-Pacheco MM, Encinas-Basurto D, Mata-Haro V, Lopez-Zavala AA, Islas-Osuna MA, Ayala-Zavala JF (2020) Synergistic mode of action of catechin, vanillic and protocatechuic acids to inhibit the adhesion of uropathogenic Escherichia coli on silicone surfaces. J Appl Microbiol 128(2):387–400
Bezerra FWF, Salazar MDLAR, Freitas LC, de Oliveira MS, dos Santos IRC, Dias MNC, de Carvalho Jr RN (2020) Chemical composition, antioxidant activity, anti-inflammatory and neuroprotective effect of Croton matourensis Aubl. leaves extracts obtained by supercritical CO2. J Supercrit Fluids 165:104992
Bhakat RK, Sen UK (2008) Ethnomedicinal plant conservation through sacred groves. Tribes and Tribals 2:55–58. Duthie JF (1903) Flora of the upper Gangetic plain, and of the adjacent Siwalik and sub-Himalayan tracts, Vol. 1. Superintendent of Government Printing
Boulekbache-Makhlouf L, Medouni L, Medouni-Adrar S, Arkoub L, Madani K (2013) Effect of solvents extraction on phenolic content and antioxidant activity of the byproduct of eggplant. Ind Crops Prod 49:668–674
Chaudhary P, Janmeda P, Docea AO, Yeskaliyeva B, Abdull Razis AF, Modu B, Sharifi-Rad J (2023) Oxidative stress, free radicals and antioxidants: potential crosstalk in the pathophysiology of human diseases. Front Chem 11:1158198
de Oliveira AM, de Freitas AFS, Costa WK, Machado JCB, Bezerra ICF, Ferreira MRA, Soares LAL (2022) Flavonoid-rich fraction of Croton blanchetianus Baill. (Euphorbiaceae) leaves: chemical profile, acute and subacute toxicities, genotoxicity and antioxidant potential. South Afr J Bot 144:238–249
Derakhshan Z, Ferrante M, Tadi M, Ansari F, Heydari A, Hosseini MS, Sadrabad EK (2018) Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juice and seeds. Food Chem Toxicol 114:108–111
Deshmukh RK, Gaikwad KK (2022) Natural antimicrobial and antioxidant compounds for active food packaging applications. Biomass Convers Biorefinery 5:1–22
Devanesan S, AlSalhi MS, Asemi NN, Al Natheer Y, Shanmuganathan R, Brindhadevi K (2023) Deciphering the fluorescence quenching potential of Croton bonplandianum leaves and detection of bioactive molecules using liquid chromatography and mass spectrometry. Chemosphere 319:138024
Duthie JF (1915) Flora of the upper gangetic plain and of the adjacent Siwalik and Sub Himalayan tracts, Vol. Superintendent of Government Printing, Calcutta, 1–3, 371
El-Ramady H, Hajdú P, Törős G, Badgar K, Llanaj X, Kiss A, Prokisch J (2022) Plant nutrition for human health: a pictorial review on plant bioactive compounds for sustainable agriculture. Sustainability 14(14):8329
Hajam YA, Rani R, Ganie SY, Sheikh TA, Javaid D, Qadri SS, Reshi MS (2022) Oxidative stress in human pathology and aging: molecular mechanisms and perspectives. Cells 11(3):552
Hooker JD (1879) Flora of British India, vol. 2. Flora of British India
Javed E, Khan HM, Shahzad Q, Shahzad Y, Yasin H, Ul-Haq Z, Khan AA (2023) Phytochemical characterization and anti-arthritic potential of Croton bonplandianus leaves extract: in-vivo and in-silico approach. Saudi Pharmac J 10:1860
Kumar I, Sharma RK (2021) Antioxidant properties in methanol extract of kernels of commonly marketed Indian fruits. Int J Fruit Sci 21(1):334–343
de Lima EJ, Alves RG, da Anunciação TA, Silva VR, Santos LDS, Soares MB, Bezerra DP (2018) Antitumor effect of the essential oil from the leaves of Croton matourensis Aubl. (Euphorbiaceae)
Liyana-Pathirana CM, Shahidi F (2005) Antioxidant activity of commercial soft and hard wheat (Triticum aestivum L.) as affected by gastric pH conditions. J Agric Food Chem 53(7):2433–2440
Mo EJ, Ahn JH, Jo YH, Kim SB, Hwang BY, Lee MK (2017) Inositol derivatives and phenolic compounds from the roots of Taraxacum coreanum. Molecules 22(8):1349
Mohan AC, Divya SR (2017) Phytochemical analysis and screening of total flavonoid, tannin and phenolic contents in Croton bonplandianum leaf and stem. World J Pharmac Res 6(4):1066–1075
Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolk RH (1995) Manual of clinical microbiology, 6th edn. Mosby Year Book, London
Nardi GM, Felippi R, DalBó S, Siqueira-Junior JM, Arruda DC, Delle Monache F, Ribeiro-do-Valle RM (2003) Anti-inflammatory and antioxidant effects of Croton celtidifolius bark. Phytomedicine 10(2–3):176–184
Ncume PV, Salau VF, Mtshali S, Olofinsan KA, Erukainure OL, Matsabisa MG (2023) Phytochemical properties of Croton gratissimus Burch (Lavender Croton) herbal tea and its protective effect against iron-induced oxidative hepatic injury. Plants 12(16):2915
Niyonizigiye I, Nkurunziza D, Ngabire D, Gitachew AT, Chun BS, Kim GD (2020) Characterization and in vitro cytotoxicity of phytochemicals from Aspilia africana obtained using green extraction techniques. S Afr J Bot 128:231–238
Olszowy M (2019) What is responsible for antioxidant properties of polyphenolic compounds from plants? Plant Physiol Biochem 144:135–143
Ordonez AAL, Gomez JD, Vattuone MA (2006) Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food Chem 97(3):452–458
Qamar M, Akhtar S, Ismail T, Wahid M, Ali S, Nazir Y, Ziora ZM (2022) Syzygium cumini (L.) Skeels extracts; in vivo anti-nociceptive, anti-inflammatory, acute and subacute toxicity assessment. J Ethnopharmacol 287:114919
Rajakaruna N, Harris CS, Towers GHN (2002) Antimicrobial activity of plants collected from serpentine outcrops in Sri Lanka. Pharm Biol 40(3):235
Rana A, Samtiya M, Dhewa T, Mishra V, Aluko RE (2022) Health benefits of polyphenols: a concise review. J Food Biochem 46(10):e14264
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26(9–10):1231–1237
Regginato A, Cunico L, Bertoncello KT, Schindler MSZ, Chitolina R, Marins K, Zanatta L (2020) Antidiabetic and hypolipidemic potential of Campomanesia xanthocarpa seed extract obtained by supercritical CO 2. Braz J Biol 81:621–631
Salatino A, Salatino MLF, Negri G (2007) Traditional uses, chemistry and pharmacology of Croton species (Euphorbiaceae). J Braz Chem Soc 18:11–33
Selowa SC, Shai LJ, Masoko P, Mokgotho MP, Magano SR (2010) Antibacterial activity of extracts of three Croton species collected in Mpumalanga region in South Africa. Afr J Tradit Complement Alternat Med 7:2
Shanmugam R, Deenadayalan P, Manigandan P (2024) Anticariogenic, antidiabetic, and toxicology evaluation of the ethanolic extract of Croton bonplandianum: an in vitro study. Cureus 16(7):87
Somit D, Priyankar DEY, Kumar CT (2013) Quantification and correlation of the bioactive phytochemicals of Croton bonplandianus leaves of Sub-Himalayan region of West Bengal. Asian J Pharm Clin Res 6(3):142–147
Sun M, Zeng W, Zhao Z (2023) Croton crassifolius Geisel.: a comprehensive review of the botany, traditional uses, phytochemistry, pharmacology, and quality control. J Ethnopharmacol 2:117352
Suresh M, Alfonisan M, Alturaiki W, Al Aboody MS, Alfaiz FA, Premanathan M, Alsagaby SA (2021) Investigations of bioactivity of Acalypha indica (L.), Centella asiatica (L.) and Croton bonplandianus (Baill) against multidrug resistant bacteria and cancer cells. J Herbal Med 28:100359
Takiguchi M, Achanzar WE, Qu W, Li G, Waalkes MP (2003) Effects of cadmium on DNA-(Cytosine-5) methyltransferase activity and DNA methylation status during cadmium-induced cellular transformation. Exp Cell Res 286(2):355–365
Truong DH, Nguyen DH, Ta NTA, Bui AV, Do TH, Nguyen HC (2019) Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of Severinia buxifolia. J Food Qual 2019(8):8
Usero J, Gonzalez-Regalado E, Gracia I (1997) Trace metals in the bivalve molluscs Ruditapes decussatus and Ruditapes philippinarum from the Atlantic Coast of Southern Spain. Environ Int 23(3):291–298
Vadlapudi V (2010) In-vitro antimicrobial activity of methanolic extract of selected Indian medicinal plant. Pharmacophore 1(3):214–219
Valarezo E, Gaona-Granda G, Morocho V, Cartuche L, Calva J, Meneses MA (2021) Chemical constituents of the essential oil from Ecuadorian endemic species Croton ferrugineus and its antimicrobial, antioxidant and α-glucosidase inhibitory activity. Molecules 26(15):4608
Vaou N, Stavropoulou E, Voidarou C, Tsigalou C, Bezirtzoglou E (2021) Towards advances in medicinal plant antimicrobial activity: a review study on challenges and future perspectives. Microorganisms 9(10):2041
Vijayamuthuramalingam UDK, Rajaram R, Kuppusamy KM, Jonnalagadda B, Arokiasamy S (2017) Anti-hyperglycemic and antioxidant potential of Croton bonplandianus. Bail fractions in correlation with polyphenol content. Iran J Basic Med Sci 20(12):1390
Wolfe K, Wu X, Liu RH (2003) Antioxidant activity of apple peels. J Agric Food Chem 51(3):609–614
Yener I (2020) Determination of antioxidant, cytotoxic, anticholinesterase, antiurease, antityrosinase, and antielastase activities and aroma, essential oil, fatty acid, phenolic, and terpenoid-phytosterol contents of Salvia poculata. Ind Crops Prod 155:112712
Zhang Y, Cai P, Cheng G, Zhang Y (2022a) A brief review of phenolic compounds identified from plants: their extraction, analysis, and biological activity. Nat Prod Commun 17(1):19345
Zhang Z, Li X, Sang S, McClements DJ, Chen L, Long J, Qiu C (2022b) Polyphenols as plant-based nutraceuticals: health effects, encapsulation, nano-delivery, and application. Foods 11(15):2189
Author Information
Department of Botany, Institute of Science, Banaras Hindu University, Varanasi, India