Polyphenols from an endemic Algerian medicinal plant: Henophyton deserti, optimization and evaluation of biological activities

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DOI: 10.1007/s42535-025-01589-0
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Keywords: n Henophyton desertin , Microwave-assisted extraction, Ultrasound-assisted extraction, Response surface methodology, Phenolic compounds


Abstract


Polyphenols from Henophyton deserti (H.deserti) were extracted using microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE). Response surface methodology (RSM) employing a Box-Behnken design was utilized. This approach optimized the extraction parameters for total phenolic concentration (TPC). The independent variables for (MAE) included power in (W), extraction duration (min), and methanol concentration (%), while amplitude (%), irradiation time (min), and methanol concentration were used for UAE. Optimal conditions for MAE were 561.18 W, 2.77 min, and 79.32% methanol concentration, while UAE required 30.13% amplitude, 16.82 min, and 79.57% methanol concentration. Predicted TPC recoveries were 80.05 µg GAE/mg (MAE) and 77.71 µg GAE/mg (UAE), which were experimentally validated with values of 79.89 ± 0.80 and 75.86 ± 0.65 µg GAE/mg, respectively. Antioxidant activity, assessed by assay (diphenyl-1-picrylhydrazyl) DPPH, hydroxyl radical scavenging, ferric reducing antioxidant power FRAP, and phenanthroline assays, confirmed MAE’s suitability for obtaining antioxidant-rich extracts. At the same time, the UAE demonstrated higher efficacy for antimicrobial activity.

n                     Henophyton desertin                  , Microwave-assisted extraction, Ultrasound-assisted extraction, Response surface methodology, Phenolic compounds


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Author Information


Bioactive Molecules and Chiral Separation Laboratory, Faculty of Exact Sciences, University Tahri Mohamed of Bechar, Bechar, Algeria