Pre harvest L-tyrosine application affect the Persian lime (Citrus latifolia Tan.) essential oils cultivated on sandy soil of Egypt


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00349-8
First Page: 816
Last Page: 824
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Keywords: Leaves, Flowers, Peels, Limonene, Linalool


Essential oil of Persian lime has biological properties that make it of interest for use in food and pharmaceutical industries. Improving plant stress tolerance is a focus of scientific research since stress and essential oil are of basic concern to maximize essential oil production under sandy soil in Egypt. Therefore, this investigation was aimed to reduce the hazardous effect of sandy soil stress conditions on Persian lime (Citrus latifolia Tan.) trees (as a natural source of essential oil) by adapting them to sandy soil by using L-tyrosine. Before bud differentiation, Persian lime trees were sprayed with two concentrations of L-tyrosine (0.2 and 0.4 g/L), in addition to spraying with distilled water (control). The essential oil composition of leaves, flowers and peels obtained from all L-tyrosine treatments and the control were investigated. The maximum values of leaf (0.5%), flower (0.4%) and peel (0.3%) essential oils were recorded along with the trees treated with 0.4 g/L of L-tyrosine. Limonene was the major component in leaf and peels essential oils, while the major component in flower essential oil, it was linalool. Oxygenated monoterpenes was the major group in leaf and flower essential oils, but the major one in peel essential oil was monoterpene hydrocarbons. The highest rates of major components and main groups were when the Persian lime trees were treated with 0.4 g/L of L-tyrosine. The addition of L-tyrosine to Persian lime trees planted in sandy soils is required because it works to adapt them under unfavorable conditions of sandy soil and improve their content of essential oils.

Leaves, Flowers, Peels, Limonene, Linalool

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Adams RP (1995) Identification of essential oil components by gas chromatography/mass spectrometry. Allured Publ Corp, Carol Stream

Ahmed AMA, Talaat IM, Khalid AK (2017) Soil Moisture and glutamic acid affect yield, volatile oil and proline contents of oregano herb (Origanum vulgare L.). Int J Bot 13:43–51

Akbarinia A, Jahanfar D, Farzad MB (2007) Effect of nitrogen fertilizer and plant density on seed yield, essential oil and oil content of Coriandrum sativum L. Iran J Med Arom Plants 22:410–419

Ashraf M, Ali Q, Iqbal Z (2006) Effect of nitrogen application rate on the content and composition of oil, essential oil and minerals in black cumin (Nigella sativa L.) seeds. J Sci Food Agric 86:871–876

Baranauskiene R, Venskutonis PR, Viskelis P, Dambrauskiene E (2003) Influence of nitrogen fertilizers on the yield and composition of thyme (Thymus vulgaris). J Agric Food Chem 51:7751–7758

Bosila H, Toaima N, Al Amier H, El-Ateeq AE (2012) Effect of some amino acids, precursors and light conditions on callus growth and silymarin content of Silybum marianum L. Al-Azhar J Agric Res 12:85–104

Buchel JA (1989) Flowering with Citrus oils. Perfum Flavor 1411:22–26

Clevenger JF (1928) Apparatus for determination of essential oil. J Am Pharm Assoc 17:345–349

Dewick PM (1997) Medicinal natural products: a biosynthetic approach. John Wiley & Sons, Chichester

El-Sherbeny MR, da Silva JAT (2013) Foliar treatment with proline and tyrosine affect the growth and yield of beetroot and some pigments in beetroot leaves. J Hortic Res 21:95–99

Fisher K, Phillips CA (2006) The effect of lemon, orange and bergamot essential oils and their components on the survival of Campylobacter jejuni, Escherichia coli O157, Li Steria Monocytogenes, Bacillus cereus and Staphylococcus aureus in vitro and in food systems. J Appl Microbiol 101:1232–1240

Foucart T (1982) Analyse factorielle. Programmatiol Sur Micro-ordinateur. Masson, Paris. ISBN-13: 978-2225764509

Gamal MK, Abd El–Wahed MSA (2005) Effect of some amino acids on growth and essential oil content of chamomile plant. Int J Agric Biol 7:376–380

Gamal KM, Tarraf AS, Balbaa L (1997) Physiological studies on the effect of some amino acids and micronutrients on growth and essential oil content in lemon grass. J Agric Sci Mans Univ 22:4229–4241

Hellal FA, Mahfouz SA, Hassan FAS (2011) Partial substitution of mineral nitrogen fertilizer by bio-fertilizer on (Anethum graveolens L.) plant. Agric Biol J North Am 4:652–660

Hildebrandt TM, Nunes Nesi A, Araújo WL, Braun HP (2015) Amino acid catabolism in plants. Mol Plant 8:1563–1579

Jiao Y, Chen Y, Ma C, Qin J, Nguyen THN, Liu D, Gan H, Ding S, Luo Z (2017) Phenylalanine as a nitrogen source induces root growth and nitrogen-use efficiency in Populus × canescens. Tree Phys 38:66–82

Khalid AK (2012) Effect of NP and foliar spray on growth and chemical compositions of some medicinal Apiaceae plants grow in arid regions in Egypt. J Soil Sci Plant Nut 12:617–632

Khalid AK (2014) Essential oil composition of some spices treated with nitrogen in arid regions. Int Food Res J 21:2305–2309

Khalid AK (2018a) Nitrogen application and different plant densities effectiveness on the productivity of parsley crop. Asian J Crop Sci 10:141–150

Khalid KA (2018b) Evaluation of black cumin oils under various nitrogen treatments. J Mater Environ Sci 9:873–878

Khalid AK, Shedeed MR (2014) Influence of kinetin on growth and biochemical accumulation in Nigella sativa plants grow under salinity stress conditions. Thai J Agric Sci 47:195–203

Khalid AK, Shedeed MR (2016) GC-MS analyses of black cumin essential oil produces with sodium chloride. Int Food Res J 23:832–836

Khalid AK, El-Gohary AE, Ahmed AMA (2018) Effect of the interaction between salicylic acid and geographical locations on grapefruit essential oil. J Essent Oil-Bear Plants 21:1594–1603

Kumar TS, Swaminathan V, Kumar S (2009) Influence of nitrogen, phosphorus and biofert ilizers on growth, yield and essential oil constituents in ratoon crop of Davana (Artemisia pallens Wall.). Electron J Environ Agric Food Chem 8:86–95

Lota M, Serra DD, Tomi FL, Jacquemond C, Casanova J (2002) Volatile components of peel and leaf oils of lemon and lime species. J Agric Food Chem 50:796–805

Maeda H, Dudareva N (2012) The shikimate pathway and aromatic amino acid biosynthesis in plants. Annu Rev Plant Biol 63:73–105

Nannapaneni R, Chalova VI, Crandall PG, Ricke S, Johnson MG, Bryan CA (2009) Campylobacter and Arcobacter species sensitivity to commercial orange oil fractions. Int J Food Microbiol 129:43–49

Omidbaigi R, Kabudani M, Khoorang M (2008a) Nitrogen fertilizer affecting herb yield, essential oil content and compositions of Agastache foeniculum purch. J Ess Oil Bear Plants 11:261–266

Omidbaigi R, Dadman B, Fattahi F (2008b) Influence of nitrogen fertilizer on the herb yield, essential oil content and composition of Tagetes minuta L. J Ess Oil Bear Plants 11:45–52

Pinto JVDC, Vieira MDC, Zarate NAH, Formagio ASN, Cardoso CA, Carnevali TO, Souza PHND (2016) Effect of soil nitrogen and phosphorus on early development and essential oil composition of Schinus terebinthifolius Raddi. J Ess Oil Bear Plants 19:247–257

Prins CL, Vieira IJC, Freitas SP (2010) Growth regulators and essential oil production. Braz J Plant Physiol 22:91–102

Rai VK (2002) Role of amino acids in plant response to stresses, Review. Biolo Planta 45:481–487

Rashmi A, Singh SB (2008) Studying the effect of nitrogen and potassium fertilizer on growth and essential oil content of Cymbopogon citratus and Vetiveria zizanioides. Ess Oil Bear Plants 11:188–193

Snedecor GW, Cochran WG (1990) Statistical methods, 11th edn. Iowa State Univ Press, Ames Iowa

Talaat IM, Youssef AA (2002) The role of the amino acids lysine and ornithine in growth and chemical constituents of basil plants. Egypt J Appl Sci 17:83–95

Talaat IM, Khattab HI, Ahmed AM (2014) Changes in growth, hormones levels and essential oil content of Ammi visnaga L. plants treated with some bioregulators. Saud J Biol Sci 21:355–365

Wahba HE, Motawe HM, Ibrahim AY (2015) Growth and chemical composition of Urtica pilulifera L. plant as influenced by foliar application of some amino acids. J Mater Environ Sci 6:499–506



The authors thank the National Research Center (NRC) for its support and facilities provided to complete this paper.

Author Information

Khalid Khalid A.
Medicinal and Aromatic Plants Department, National Research Centre, Cairo, Egypt
Ahmed Aisha M. A.
2 Botany Department, National Research Centre, Cairo, Egypt