Keywords:
L-amino acid oxidase,
Aspergillus terreus
, Kinetic model, Immobilization
L-Amino acid oxidase (LAAO) has significant importance in different biotechnological sectors. It catalyzes the stereospecific oxidative deamination of L- amino acids into α-keto acid, hydrogen peroxide, and ammonia. In this study, Aspergillus terreus MZ769058 has been isolated as new fungal strain for producing LAAO with maximum activity (246.5-unit ml−1). The production kinetic curve has confirmed its non-growth associated behaviour. Logistic equation and Luedeking-Piret equations have been employed for developing suitable growth and production kinetic model. The stability of LAAO has been improved up to 73% after carrying immobilization with sodium alginate entrapment method. The optimization of conditions for immobilization has improved the activity of enzyme LAAO upto 336.2-unit ml−1 at the optimized pH value (5.5), temperature (50 °C), reaction time (15 min). The immobilized beads of LAAO could be effectively used for fulfilling their great demand in industrial sectors in future.
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The authors are grateful to the Department of Biosciences and Biotechnology, Banasthali Vidyapith, for providing infrastructural facilities and moral support for completing the research work successfully.