Production of L-amino acid oxidase from new fungal isolate Aspergillus terreus MZ769058 and optimization of their immobilization parameters

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Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00457-5
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Keywords: L-amino acid oxidase, n Aspergillus terreusn , Kinetic model, Immobilization


Abstract


L-Amino acid oxidase (LAAO) has significant importance in different biotechnological sectors. It catalyzes the stereospecific oxidative deamination of L- amino acids into α-keto acid, hydrogen peroxide, and ammonia. In this study, Aspergillus terreus MZ769058 has been isolated as new fungal strain for producing LAAO with maximum activity (246.5-unit ml−1). The production kinetic curve has confirmed its non-growth associated behaviour. Logistic equation and Luedeking-Piret equations have been employed for developing suitable growth and production kinetic model. The stability of LAAO has been improved up to 73% after carrying immobilization with sodium alginate entrapment method. The optimization of conditions for immobilization has improved the activity of enzyme LAAO upto 336.2-unit ml−1 at the optimized pH value (5.5), temperature (50 °C), reaction time (15 min). The immobilized beads of LAAO could be effectively used for fulfilling their great demand in industrial sectors in future.


L-amino acid oxidase, n              Aspergillus terreusn            , Kinetic model, Immobilization


References


Aamir S, Sutar S, Singh SK, Baghela A (2015) A rapid and efficient method of fungal genomic DNA extraction, suitable for PCR-based molecular methods. Plant PatholQuar 5(2):74–81


Ahmed NE, El Shamy AR, Awad HM (2020) Optimization and immobilization of amylase produced by Aspergillus terreus using pomegranate peel waste. Bull Natl Res Centre 44(1):1–12


Akilandeswari P, Pradeep BV (2017) Aspergillus terreus KMBF1501 a potential pigment producer under submerged fermentation. Int J Pharm Pharm Sci 9:38–43


Al-Adhami AJ, Bryjak J, Greb-Markiewicz B, Peczynska-Czoch W (2002) Immobilization of wood-rotting fungi laccases on modified cellulose and acrylic carriers. Process Biochem 37(12):1387–1394


Ande SR, Fussi H, Knauer H, Murkovic M, Ghisla S, Frohlich KU, Macheroux P (2008) Induction of apoptosis in yeast by L-amino acid oxidase from the Malayan pit viper Calloselasmarhodostoma. Yeast 25:349–357


Atalla MM, El-Khrisy EAM, Youssef YA, Mohamed AA (2011) Production of textile reddish brown dyes by fungi. Malays J Microbiol 7(1):33–40


Awad GE, Abd El Aty AA, Shehata AN, Hassan ME, Elnashar MM (2016) Covalent immobilization of microbial naringinase using novel thermally stable biopolymer for hydrolysis of naringin. 3 Biotech 6(1):1–10


Brearley GM, Price CP, Atkinson T, Hammond PM (1994) Purification and partial characterization of a broad-range L-amino acid oxidase from Bacillus carotarum 2Pfa isolated from soil. Appl Microbiol Biotechnol 41(6):670–676


Calvo AM, Wilson RA, Bok JW, Keller NP (2002) Relationship between secondary metabolism and fungal development. Microbiol Mol Biol Rev 66(3):447–459


Campillo-Brocal JC, Lucas-Elio P, Sanchez-Amat A (2015) Distribution in different organisms of amino acid oxidases with FAD or a Quinone as cofactor and their role as antimicrobial proteins in marine bacteria. Mar Drugs 13:7403–7418


Castellano F, Molinier-Frenkel V, Castellano F, Molinier-Frenkel V, Castellano F, Molinier-Frenkel V (2017) An overview of L-amino acid oxidase functions from bacteria to mammals: focus on the immunoregulatory phenylalanine oxidase IL4I1. Molecules 22:12


Ciscotto P, De Avila RM, Coelho EAF, Oliveira J, Diniz CG, Farias LM, Chavez-Olortegui C (2009) Antigenic, microbicidal and antiparasitic properties of an l-amino acid oxidase isolated from Bothrops jararaca snake venom. Toxicon 53(3):330–341


Datta S, Christena LR, Rajaram YRS (2013) Enzyme immobilization: an overview on techniques and support materials. 3 Biotech 3(1):1–9


Davis MA, Askin MC, Hynes MJ (2005) Amino acid catabolism by an areA-regulated gene encoding an L-amino acid oxidase with broad substrate specificity in Aspergillus nidulans. Appl Environ Microbiol 71(7):3551–3555


Devi B, Unni BG, Wann SB, Samanta R (2012) Immobilization of partially purified alpha-amylase enzyme produced by a soil born Bacillus sp. Adv Appl Sci Res 3(5):2739–2744


Don MM, Shoparwe NF (2010) Kinetics of hyaluronic acid production by Streptococcus zooepidemicus considering the effect of glucose. Biochem Eng J 49(1):95–103





Du XY, Clemetson KJ (2002) Snake venom L-amino acid oxidases. Toxicon 40(6):659–665


Duerre JA, Chakrabarty SUBHAS (1975) L-amino acid oxidases of Proteus rettgeri. J Bacteriol 121(2):656–663





El-Sayed AS, Shindia AA, Zaher Y (2012) L-amino acid oxidase from filamentous fungi: screening and optimization. Ann Microbiol 62(2):773–784


Geiser DM, Klich MA, Frisvad JC, Peterson SW, Varga J, Samson RA (2007) The current status of species recognition and identification in Aspergillus. Stud Mycol 59(1):1–10





Geueke B, Hummel W (2003) Heterologous expression of Rhodococcusopacus L-amino acid oxidase in Streptomyces lividans. Protein Expr Purif 28(2):303–309


Gomez D, Lucas-Elío P, Sanchez-Amat A, Solano F (2006) A novel type of lysine oxidase: l-lysine-ε-oxidase. Biochim Biophys Acta BBA Proteins Proteomics 1764(10):1577–1585


Gonzalez-Saiz JM, Pizarro C (2001) Polyacrylamide gels as support for enzyme immobilization by entrapment. Effect of polyelectrolyte carrier, pH and temperature on enzyme action and kinetics parameters. Eur Polym J 37(3):435–444


Hossain GS, Li J, Shin HD, Du G, Liu L, Chen J (2014) L-amino acid oxidases from microbial sources: types, properties, functions, and applications. Appl Microbiol Biotechnol 98(4):1507–1515. https://doi.org/10.1007/s00253-013-5444-2


Karahalil E, Demirel F, Evcan E, Germeç M, Tari C, Turhan I (2017) Microparticle-enhanced polygalacturonase production by wild type Aspergillus sojae. 3 Biotech 7(6):1–9


Kasai K, Nakano M, Ohishi M, Nakamura T, Miura T (2021) Antimicrobial properties of L-amino acid oxidase: biochemical features and biomedical applications. Appl Microbiol Biotechnol 105(12):4819–4832


Kawaguti HY, Manrich E, Sato HH (2006) Production of isomaltulose using Erwinia sp. D12 cells: culture medium optimization and cell immobilization in alginate. Biochem Eng J 29(3):270–277


Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874


Kusakabe H, Kodama K, Machida H, Midorikawa Y, Kuninaka A, Misono H, Soda K (1979) Occurrence of a novel enzyme, L-lysine oxidase with antitumor activity in culture extract of Trichoderma viride. Agric Biol Chem 43(2):337–343


Kusakabe H, Kodama K, Kuninaka A, Yoshino H, Misono H, Soda K (1980) A new antitumor enzyme, L-lysine alpha-oxidase from Trichoderma viride. Purification and enzymological properties. J Biol Chem 255(3):976–981


Lee DH, Park CH, Yeo JM, Kim SW (2006) Lipase immobilization on silica gel using a cross-linking method. J Ind Eng Chem 12(5):777–782


Lu QM, Wei Q, Jin Y, Wei JF, Wang WY, Xiong YL (2002) L-amino acid oxidase from Trimeresurusjerdonii snake venom: purification, characterization, platelet aggregation-inducing and antibacterial effects. J Nat Toxins 11(4):345–352


Luedeking R, Piret EL (2000) A kinetic study of lactic acid fermentation. The batch process at controlled pH. Biotechnol Bioeng 67(6):636–644


Mai-Prochnow A, Evans F, Dalisay-Saludes D, Stelzer S, Egan S, James S, Kjelleberg S (2004) Biofilm development and cell death in the marine bacterium Pseudoalteromonas tunicata. Appl Environ Microbiol 70(6):3232–3238


Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31(3):426–428


Mogharabi M, Nassiri-Koopaei N, Bozorgi-Koushalshahi M, Nafissi-Varcheh N, Bagherzadeh G, Faramarzi MA (2012) Immobilization of laccase in alginate-gelatin mixed gel and decolorization of synthetic dyes. Bioinorg Chem Appl. https://doi.org/10.1155/2012/823830


Nei M, Kumar S (2000) Molecular evolution and phylogenetics. Oxford University Press, USA


Peng Xu, Pan C, Cui G, Wei ChunYan, Wang L, Li Y, Li X, Huang S (2020) Enhancement of L-amino acid oxidase production by Bacillus subtilis HLZ-68 with oxygen-vector and asymmetric degradation of DL-arginine to D-arginine. Biotechnol Biotechnol Equip 34(1):1273–1279





Riaz A, Ansari B, Siddiqui A, Ahmed S, Naheed SUL, Qader SA (2015) Immobilization of α-amylase in operationally stable calcium-alginate beads: a cost effective technique for enzyme aided industrial processes. Int J Biotechnol Res 3(5):081–086


Rodrigues RS, da Silva JF, França JB, Fonseca FP, Otaviano AR, Silva FH, Rodrigues VM (2009) Structural and functional properties of Bp-LAAO, a new L-amino acid oxidase isolated from bothropspauloensis snake venom. Biochimie 91(4):490–501


Rosa CC, Cruz HJ, Vidal M, Oliva AG (2002) Optical biosensor based on nitrite reductase immobilized in controlled pore glass. Biosens Bioelectron 17(1–2):45–52


Ruiz-Herrera J, Starkey RL (1969) Dissimilation of methionine by fungi. J Bacteriol 99(2):544–551


Saithi S, Borg J, Nopharatana M, Tongta A (2016) Mathematical modeling of biomass and enzyme production kinetics by Aspergillus niger in solid-state fermentation at various temperatures and moisture contents. J Microb Biochem Technol 8(2):123–130


Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425


Samel M, Tonismagi K, Ronnholm G, Vija H, Siigur J, Kalkkinen N, Siigur E (2008) L-amino acid oxidase from Najanajaoxiana venom. Comp Biochem Physiol B: Biochem Mol Biol 149(4):572–580


Samson RA, Noonim P, Meijer M, Houbraken JAMP, Frisvad JC, Varga J (2007) Diagnostic tools to identify black Aspergilli. Stud Mycol 59(1):129–145


Sattar H, Aman A, Qader SAU (2018) Agar-agar immobilization: an alternative approach for the entrapment of protease to improve the catalytic efficiency, thermal stability and recycling efficiency. Int J Biol Macromol 111:917–922


Saurina J, Hernandez-Cassou S, Fàbregas E, Alegret S (1998) Potentiometric biosensor for lysine analysis based on a chemically immobilized lysine oxidase membrane. Anal Chim Acta 371(1):49–56


Sheldon RA, van Pelt S (2013) Enzyme immobilization in biocatalysis: why what and how. Chem Soc Rev 42(15):6223–6235


Shen Q, Yang R, Hua X, Ye F, Zhang W, Zhao W (2011) Gelatin-templated biomimetic calcification for β-galactosidase immobilization. Process Biochem 46(8):1565–1571


Singh BD (2009) Biotechnology expanding horizons-amylase immobilization on the silica nanoparticles for cleaning performance towards starch soils in laundry detergents. J Mol Catal B 74:1–5


Talekar S, Chavare S (2012) Optimization of immobilization of α-amylase in alginate gel and its comparative biochemical studies with free α-amylase. Recent Res Sci Technol 4(2):151


Teixeira MF, Martins MS, Da Silva JC, Kirsch LS, Fernandes OC, Carneiro AL, Durán N (2012) Amazonian biodiversity: pigments from Aspergillus and Penicillium-characterizations, antibacterial activities and their toxicities. Curr Trends Biotechnol Pharm 6(3):300–311


Thom C, Raper KB (1945) A manual of the Aspergilli. Williams and W. Wilkins Co., Baltimore, USA


Trelles JA, Rivero CW (2020) Whole cell entrapment techniques. Immobilization of enzymes and cells. Springer, New York, NY, pp 385–394


Treshalina HM, Lukasheva EV, Sedakova LA, Firsova GA, Guerassimova GK, Gogichaeva NV, Berezov TT (2000) Anticancer enzyme L-lysine α-oxidase. Appl Biochem Biotechnol 88(1):267–273


Ueda N, Shah SV (1992) Endonuclease-induced DNA damage and cell death in oxidant injury to renal tubular epithelial cells. J Clin Invest 90(6):2593–2597


Van Boven A, Tan PST, Konings WN (1988) Purification and characterization of a dipeptidase from Streptococcus cremoris Wg2. Appl Environ Microbiol 54(1):43–49


Vatansever F, De Melo WC, Avci P, Vecchio D, Sadasivam M, Gupta A, Chandran R, Karimi M, Parizotto NA, Yin R, Tegos GP, Hamblin MR (2013) Antimicrobial strategies centered around reactive oxygen species–bactericidal antibiotics, photodynamic therapy, and beyond. FEMS Microbiol Rev 37(6):955–989


Wei JF, Yang HW, Wei XL, Qiao LY, Wang WY, He SH (2009) Purification, characterization, and biological activities of the L-amino acid oxidase from Bungarus fasciatus snake venom. Toxicon 54(3):262–271


Wu X, Jiang S, Zhang M, Luo S, Li X, Pan L, Liu M (2015) A new method studying the kinetics of l-lactic acid production by pellets Rhizopus oryzae in semi-continuous fermentation. Ann Microbiol 65(3):1473–1480


Yang H, Johnson PM, Ko KC, Kamio M, Germann MW, Derby CD, Tai PC (2005) Cloning, characterization, and expression of escapin, a broadly antimicrobial FAD-containing L-amino acid oxidase from the ink of the sea hare Aplysia californica. J Exp Biol 208(18):3609–3622

 


Acknowledgements


The authors are grateful to the Department of Biosciences and Biotechnology, Banasthali Vidyapith, for providing infrastructural facilities and moral support for completing the research work successfully.


Author Information


Yadav Monika
Department of Bioscience & Biotechnology, Banasthali Vidyapith, Tonk, India

Singh Priyanka
Institute of Allied Medical Science and Technology, NIMS University, Jaipur, India
priyay20@gmail.com