Production of L-amino acid oxidase from new fungal isolate Aspergillus terreus MZ769058 and optimization of their immobilization parameters


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00457-5
First Page: 851
Last Page: 863
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Keywords: L-amino acid oxidase, Aspergillus terreus , Kinetic model, Immobilization


L-Amino acid oxidase (LAAO) has significant importance in different biotechnological sectors. It catalyzes the stereospecific oxidative deamination of L- amino acids into α-keto acid, hydrogen peroxide, and ammonia. In this study, Aspergillus terreus MZ769058 has been isolated as new fungal strain for producing LAAO with maximum activity (246.5-unit ml−1). The production kinetic curve has confirmed its non-growth associated behaviour. Logistic equation and Luedeking-Piret equations have been employed for developing suitable growth and production kinetic model. The stability of LAAO has been improved up to 73% after carrying immobilization with sodium alginate entrapment method. The optimization of conditions for immobilization has improved the activity of enzyme LAAO upto 336.2-unit ml−1 at the optimized pH value (5.5), temperature (50 °C), reaction time (15 min). The immobilized beads of LAAO could be effectively used for fulfilling their great demand in industrial sectors in future.

L-amino acid oxidase, 
              Aspergillus terreus
            , Kinetic model, Immobilization

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The authors are grateful to the Department of Biosciences and Biotechnology, Banasthali Vidyapith, for providing infrastructural facilities and moral support for completing the research work successfully.

Author Information

Yadav Monika
Department of Bioscience & Biotechnology, Banasthali Vidyapith, Tonk, India

Singh Priyanka
Institute of Allied Medical Science and Technology, NIMS University, Jaipur, India