Seeds of Terminalia chebula, collected from Punjab and adjoining states of Haryana, Himachal Pradesh and Jammu and Kashmir were subjected to different pre-sowing seed treatments to overcome dormancy. The fruits from different locations had different shapes and sizes but the taste and odour was same. The fruits and seeds from Haryana were big and heavier than other locations. The seeds of Punjab and Haryana exhibited higher germination. The pre-sowing treatment with 50% concentrated sulphuric acid for 2 min was most effective to enhance germination. Seed vigour and seedling recovery per cent declined with storage and enhanced with treatment of concentrated sulphuric acid followed by boiling water, cold water and cowdung slurry treatments. The results for micro-propagation of T. chebula revealed that MS + BAP (6-benzylaminopurine 1 mg/l) + kinetin (0.5 mg/l) + GA3 (0.5 mg/l) + charcoal (0.4 g/l) media promoted maximum bud break (71.63%), mean shoot length (25.00 mm) and number of shoots per explant (1.60).
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The facilities provided by plant tissue culture laboratory, School of Agricultural Biotechnology, PAU, Ludhiana for the research experiment are duly acknowledged.