Proximate analysis, preliminary phytochemical screening and characterization of compounds by GC–MS from “Cycas revoluta


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-021-00338-3
First Page: 756
Last Page: 762
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Keywords: Cycas revoluta , Proximate analysis, Phytochemical screening, GC–MS, Palmitoleic acid


The author conducted a comparative analysis to investigate the nutritive activities through proximate analysis and phytoconstituents of Cycas revoluta with different solvent extracts. For this purpose, the AOAC Internationals (Association of Official Agricultural Chemists) and Standard Lowry methods for protein estimation were used to forecast nutritional components such as ash%, moisture%, crude protein%, crude fat%, crude fibers%, and carbohydrate. Plants consist of a variety of phytochemicals that have variable solvation capabilities based on their polarity. The author also quantifies the effects of different solvents on the phytochemical profile and characterization of several volatile bioactive chemicals of Cycas revoluta. For phytochemical screening, a sequential extraction was performed utilizing several solvent systems, including methanol (MeOH), chloroform, carbon tetrachloride, hexane, and ethyl acetate (EtAc). The hyphenated approach gas chromatography-mass spectrometry (GC–MS) was used to evaluate the volatile components. The ash content was found to be high which indicates the presence of various inorganic elements. The carbohydrates were found in more amount, followed by Protein and lipid. According to the percent yield of various extracts, certain bioactive phytochemicals are much more available in more polar solvents such as MeOH, others are soluble in intermediate solvents such as EtAc, and most non-polar organic compounds may be abstracted utilizing non-polar agents such as nH. GC–MS analysis revealed the presence of Palmitoleic acid, Phytol, Campesterol, ( +)-à-Tocopherol, Neophytadiene, á-Sitosterol and Ethyl iso-allocholate in the extract of n-hexane, ethyl acetate, and methanol which might largely contribute in the antioxidant and antimicrobial activity.

                Cycas revoluta              , Proximate analysis, Phytochemical screening, GC–MS, Palmitoleic acid

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The authors would like to thank Ms. Mital Nakrani and Gujarat Biotechnology Research Centre, Gandhinagar to provide GC-MS as a part of shared lab facilities to carry out this research work. Further we would like to convey our gratitude to department of chemistry, PSSHDA and HVHP institute of Post graduate studies and research for Lab facilities.

Author Information

Sharma Deepak Kumar
Department of Chemistry, HVHP Institute of Post Graduate Studies and Research, Sarva Vidhyala Campus, Kadi, India
Shah K. R.
Department of Biotechnology, Pramukh Swami Science and H.D Patel Arts College, Kadi, India