Vegetos
(An International Journal of Plant Research & Biotechnology)
Reduced hyperhydricity and enhanced adventitious shoot induction, proliferation, and regeneration of Gynura divaricata (L.) DC.
*Article not assigned to an issue yet
Zeng Yujie, Xiong Yuping, Li Jianrong, Wu Kunlin, Zeng Songjun, Silva Jaime A. Teixeira da, Ma Guohua
Research Articles | Published: 13 April, 2025
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Keywords: Shoot proliferation coefficient (SPC), Hyperhydricity, Adventitious shoot induction, Leaf explants, Transplantation
Abstract
Gynura divaricata (L.) DC., a herb with economic, ecological, and medical importance, is widely cultivated in tropical and subtropical areas of Southeast Asia. G. divaricata can bloom and set seeds, but only a small number of seeds form and very few are viable, so seedlings are rarely employed for propagation purposes. In this study, callus and adventitious shoots were induced from leaves. Adventitious shoot propagation is reported for the first time for this species. The optimal medium for direct induction of adventitious shoots from leaf explants was Murashige and Skoog (MS) medium containing 4.0 mg L− 1 thidiazuron (TDZ), forming 7.78 adventitious shoot buds per explant. When 2.0 mg L− 1 TDZ and 0.5 mg L− 1 α-naphthaleneacetic acid (NAA) or 2.0 mg L− 1 6-benzyladenine (BA) and 0.5 mg L− 1 NAA were added to MS medium, callus was induced initially, followed by 9.78 or 8.87 adventitious shoot buds (per explant), which developed from callus. Adventitious shoots could not be induced by 2,4-dichlorophenoxyacetic acid, NAA or kinetin. The addition of L-ascorbic acid (AsA) to MS medium reduced the level of hyperhydricity of shoots and plantlets. The optimal medium for shoot proliferation was MS medium with 4.0 mg L− 1 BA and 50 mg L− 1 AsA. Optimal rooting of G. divaricata was achieved on MS medium with 0.5 mg L− 1 indole-3-butyric acid, with 97.33% of shoots forming roots within 1 week. The optimal substrate for transplantation was a mixture of peat and vermiculite or peat and fine sand (1:1, v/v). After 3 weeks, the survival percentage of transplanted plantlets was 98.15%.
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Author Information
South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China