Regeneration response of carnation cultivars in response of silver nanoparticles under in vitro conditions

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-019-00074-9
First Page: 11
Last Page: 20
Views: 1886

Keywords: Carnation, Silver nanoparticles, In vitro, Antioxidant, Phenolic, Flavonoid


The purpose of this study was to analyze the effect of chemically synthesized silver nanoparticles (AgNPs) on in vitro regeneration of carnation cultivars cv. Noblessa, cv. Antigua and cv. Mariposa. Number of shoots/explant of cv. Noblesse and cv. Antigua significantly increased at 6 mg/L AgNPs (average 7.33 shoots per explant) when supplemented in MS media. While cv. Mariposa showed highest regeneration rate at 8 mg/L (average 10 shoots per explant). High concentration of AgNPs (12 mg/L) in the medium enhanced rooting response, number of roots/plant, and root length as compared with control. The fresh and dry weight of regenerated plants significantly (P < 0.05) increased at 6 mg/L. DPPH based free radical scavenging activity, total antioxidant activity and reducing power potential of regenerated plants varied depending upon concentration of AgNPs in the media. To find non-enzymatic antioxidants to combat oxidative damage, total phenolics and flavonoids were also determined in the regenerated plants. The study concludes that metallic nanoparticles has significant effect on in vitro growth of carnation cultivars however concentration dependent. Furthermore, nanoparticles can be effectively used for increased in vitro shoot multiplication and regeneration of floriculture plants.

Carnation, Silver nanoparticles, In vitro, Antioxidant, Phenolic, Flavonoid

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Author Information

Zia Muhammad
Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan
Yaqoob Kamran
Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan

Mannan Abdul
Department of Pharmacy, COMSATS University, Abbotabad, Pakistan

Nisa Sobia
Department of Microbiology, University of Haripur, Haripur, Pakistan

Raza Ghulam
Division of Plant Biotechnology, NIBGE, Faisalabad, Pakistan

Rehman Riaz ur
Department of Floriculture and Horticulture, Government of Punjab, Lahore, Pakistan