Keywords: Antioxidant enzymes, Cadmium and nickel chloride stress, Germination, n Guizotia abyssinican , Proline
Niger (Guizotia abyssinica (L.f.) Cass) is an incredibly useful but underutilized oil crop commonly grown in East Africa, mainly in Ethiopia, and the Indian subcontinent. Various abiotic stresses of the present-day environment significantly reduce crop productivity. Of them, heavy-metal stress is one of the major causes of osmotic stress that leads to oxidative stress. The present study aims to elucidate and understand the influence of nickel and cadmium chloride stress on seed germination, chlorophyll content, protein content, hydrogen peroxide production, proline accumulation, and antioxidant enzyme activity in niger. The germination rate of sterilized seeds exposed to 0, 25, 50, 75, 100, and 150 ppm of nickel and cadmium chloride was recorded 24 h after treatments. Four-week-old seedlings were also treated with heavy metals and biochemical observations were recorded on the 15th day after treatment. A direct relationship was noted between heavy metal stress and the hydrogen peroxide and proline content of niger. The extent of oxidative stress was significantly higher, as compared to the control, in all the treatments. The majority of the antioxidants exhibited a significantly negative relationship with chlorophyll and protein content of niger. Furthermore, a higher concentration of heavy metal stress results in decreased germination rate, chlorophyll content and triggers antioxidant enzyme activity. Thus, oxidative stress in niger caused by a high level of nickel and cadmium, which could result in membrane damage via the production of reactive oxygen species was compensated by the active defense mechanism via high proline accumulation and antioxidant enzyme activity.
Ahmad A, Siddiqi TO, Iqbal M (2011) Medicinal plants in changing environment. Capital Publishing Company, New Delhi, India (ISBN: 81/85589-14-3)
Akhtar T, Zia-ur-Rehman M, Naeem A, Nawaz R, Ali S, Murtaza G, Rizwan M (2017) Photosynthesis and growth response of maize (Zea mays L.) hybrids exposed to cadmium stress. Environ Sci Pollut Res 24(6):5521–5529. https://doi.org/10.1007/s11356-016-8246-0
Alemayehu T (2001) The impact of uncontrolled waste disposal on surface water quality in Addis Ababa Ethiopia. SINET Ethiop J Sci 24(1):93–104. https://doi.org/10.4314/sinet.v24i1.18177
Alia BB, Pardha Saradhi P, Mohanty P (1993) Proline in relation to free radical production in seedlings of Brassica juncea raised under sodium chloride stress. Plant Soil 155(156):497500
Anjum NA, Umar S, Iqbal M (2014) Assessment of cadmium accumulation, toxicity, and tolerance in Brassicaceae and Fabaceae plants—implications for phytoremediation. Environ Sci Pollut Res 21(17):10286–10293. https://doi.org/10.1007/s11356-014-2889-5
Anjum SA, Tanveer M, Hussain S, Bao M, Wang L, Khan I, Shahzad B (2015) Cadmium toxicity in Maize (Zea mays L.): consequences on antioxidative systems, reactive oxygen species and cadmium accumulation. Environ Sci Pollut Res 22(21):17022–17030. https://doi.org/10.1007/s11356-015-4882-z
Ansari MKA, Ahmad A, Umar S, Zia MH, Iqbal M, Owens G (2015) Genotypic variation in phytoremediation potential of Indian mustard exposed to nickel stress: A hydroponic study. Int J Phytorem 17:135–144. https://doi.org/10.1080/15226514.2013.862206
Aref IM, Khan PR, Khan S, El-Atta H, Ahmed AI, Iqbal M (2016) Modulation of antioxidant enzymes in Juniperus procera needles in relation to habitat environment and dieback incidence. Trees Struct Funct 30:1669–1681. https://doi.org/10.1007/s00468-016-1399-0
Arnon DI (1949) Copper enzymes in isolated chloroplasts Polyphenoloxidase in Beta vulgaris. Plant Physiol 24(1):1–15. https://doi.org/10.1104/2Fpp.24.1.1
Arora M, Kiran B, Rani S, Rani A, Kaur B, Mittal N (2008) Heavy metal accumulation in vegetables irrigated with water from different sources. Food Chem 111(4):811–815. https://doi.org/10.1016/j.foodchem.2008.04.049
Asada K, Takahashi M (1987) Production and scavenging of active oxygens in chloroplasts. Photoinhibition 1987:227–287
Ashraf M (2009) Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnol Adv 27(1):84–93
Azad HN, Shiva AH, Malekpour R (2011) Toxic effects of lead on growth and some biochemical and ionic parameters of sunflower (Helianthus annuus L.) seedlings. Curr Res J Biol Sci 3:398–403
Bagheri R, Bashir H, Ahmad J, Iqbal M, Qureshi MI (2015) Spinach (Spinacia oleracea L.) modulates its proteome differentially in response to salinity, cadmium and their combination stress. Plant Physiol Biochem 97:235–245. https://doi.org/10.1016/j.plaphy.2015.10.012
Baker AJM, Brooks R (1989) Terrestrial higher plants which hyperaccumulate metallic elements A review of their distribution, ecology and phytochemistry. Biorecovery 1(2):81–126
Bashir H, Qureshi MI, Ibrahim AM, Iqbal M (2015) Chloroplast and photosystems: impact of cadmium and iron deficiency. Photosynthetica 53(3):321–335. https://doi.org/10.1007/s11099-015-0152-z
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39(1):205–207. https://doi.org/10.1007/BF00018060
Chakraborty U, Pradhan B (2012) Oxidative stress in five wheat varieties (Triticum aestivum L.) exposed to water stress and study of their antioxidant enzyme defense system, water stress responsive metabolites and H2O2 accumulation. Braz J Plant Physiol 24:117–130
Chandlee JM, Scandalios JG (1984) Analysis of variants affecting the catalase developmental program in maize scutellum. Theor Appl Genet 69(1):71–77. https://doi.org/10.1007/BF00262543
DaCosta M, Huang B (2007) Changes in antioxidant enzyme activities and lipid peroxidation for bentgrass species in response to drought stress. J Am Soc Hortic Sci 132(3):319–326
De Maria S, Puschenreiter M, Rivelli AR (2013) Cadmium accumulation and physiological response of sunflower plants to Cd during the vegetative growing cycle. J Plant Soil Environ 59:254–261. https://doi.org/10.17221/788/2012-PSE
Dirbaba N, Yan X, Wu H, Colebrooke L, Wang J (2018) Occurrences and ecotoxicological risk assessment of heavy metals in surface sediments from Awash River Basin. Ethiop Water 10(5):535. https://doi.org/10.3390/w10050535
Dutta PC, Helmersson S, Kebedu E, Alemaw G, Appelqvist LÅ (1994) Variation in lipid composition of niger seed (Guizotia abyssinica Cass) samples collected from different regions in Ethiopia. J Am Oil Chem Soc 71(8):839–843. https://doi.org/10.1007/BF02540459
Gajewska E, Skłodowska M, Słaba M, Mazur J (2006) Effect of nickel on antioxidative enzyme activities, proline and chlorophyll contents in wheat shoots. Biol Plant 50(4):653–659. https://doi.org/10.1007/s10535-006-0102-5
Hossain Z, Mandal AKA, Datta SK, Biswas AK (2007) Development of NaCl-tolerant line in Chrysanthemum morifolium Ramat through shoot organogenesis of selected callus line. J Biotechnol 129(4):658–667
Husen A, Iqbal M, Khanam N, Aref IM, Sohrab SS, Masresha G (2019) Modulation of salt-stress tolerance of Niger (Guizotia abyssinica), an oilseed plant, by application of salicylic acid. J Environ Biol 40(1):96–104
Iqbal M, Khudsar T (2000) Heavy metal stress and forest cover: Plant performance as affected by cadmium toxicity. In: Kohli RK, Singh HP, Vij SP, Dhir KK, Batish DR, Khurana DK (eds) Man and Forests: 85–112, DNES, IUFRO, ISTS & Punjab University. Chandigarh, India
Iqbal M, Srivastava PS, Siddiqi TO (2000) Environmental hazards: plants and people. CBS Publishers, New Delhi, India (ISBN: 81-239-0644-7)
Júnior CAL, Mazzafera P, Arruda MAZ (2014) A comparative ionomic approach focusing on cadmium effects in sunflowers (Helianthus annuus L.). Environ Exp Bot 107:180–186. https://doi.org/10.1016/j.envexpbot.2014.06.002
Kearsey MJ, Pooni HS (1996) The Genetical analysis of quantitative traits. Chapman and Hall, Birmingham, UK
Leonard SS, Harris GK, Shi X (2004) Metal-induced oxidative stress and signal transduction. Free Rad Biol Med 37:1921–1942. https://doi.org/10.1016/j.freeradbiomed.2004.09.010
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Estimations of protein with the folin phenol reagent. J Biol Chem 193:262–275
Mahmood T, Islam KR, Muhammad S (2007) Toxic effects of heavy metals on early growth and tolerance of cereal crops. Pak J Bot 39(2):451–462
Matysik J, Bhalu B, Mohanty P (2002) Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Curr Sci 82(5):525–532
Mehindirata S, Ali ST, Mahmooduzzafar S, T.O. & Iqbal, M. (1999) Cadmium-induced changes in foliar responses of Solanum melongena L. Phytomorphology 49:295–302
Mehindirata S, Mahmooduzzafar S, T.O. & Iqbal, M. (2000) Cadmium-induced changes in growth and structure of root and stem of Solanum melongena L. Phytomorphology 50:243–251
Mekonnen KN, Ambushe AA, Chandravanshi BS, Redi-Abshiro M, McCrindle RI (2014) Assessment of potentially toxic elements in Swiss chard and sediments of Akaki River Ethiopia. Toxicol Environ Chem 96(10):1501–1515. https://doi.org/10.1080/02772248.2015.1025788
Nagella P, Hosakatte NM, Ravishankar KV, Hahn EJ, Paek KY (2008) Analysis of genetic diversity among Indian niger [Guizotia abyssinica (L. f.) Cass.] cultivars based on randomly amplified polymorphic DNA markers. Electron J Biotechnol 11(1):140–144. https://doi.org/10.4067/S0717-34582008000100014
Naik HNK, Devaraj RV (2016) Effect of salinity stress on antioxidant defense system of Niger (Guizotia abyssinica Cass.). Am J Plant Sci 7(6):980–990
Naik KH, Devaraj VR (2017) Induction of antioxidant system in niger (Guizotia abyssinica Cass.) under drought stress. Af J Agric Res 12(41):3037–3044. https://doi.org/10.5897/AJAR2017.12709
Pandey N, Sharma CP (2002) Effect of heavy metals Co2+, Ni2+ and Cd2+ on growth and metabolism of cabbage. Plant Sci 163:753–758. https://doi.org/10.1016/S0168-9452(02)00210-8
Parida BK, Chhibba IM, Nayyar VK (2003) Influence of nickel-contaminated soils on fenugreek (Trigonella corniculata L.) growth and mineral composition. Sci Hort 98:113–119. https://doi.org/10.1016/S0304-4238(02)00208-X
Patil PP, Ghane SG, Barmukh RB, Teixeira da Silva JA, Nikam TD (2010) Differential response of niger (Guizotia abyssinica Cass.) cultivars to salinity stress in relation to seed germination, oxidative stress, osmotic adjustment and antioxidant enzyme activities. Plant Stress 4(1):56–63
Prasad, M. N., & Strzalka, K. (Eds.). (2013). Physiology and biochemistry of metal toxicity and tolerance in plants. Springer Science & Business Media.
Rahman S, Iqbal M, Husen A (2023) Medicinal Plants and Abiotic Stress: An Overview. Their Response to Abiotic Stress, Medicinal Plants, pp 1–34
Ramadan MF, Morsel JT (2002) Proximate neutral lipid composition of niger. Czech J Food Sci 20:98–104
Recatalá L, Sánchez J, Arbelo C, Sacristán D (2010) Testing the validity of a Cd soil quality standard in representative Mediterranean agricultural soils under an accumulator crop. Sci Total Environ 409(1):9–18. https://doi.org/10.1016/j.scitotenv.2010.09.021
Rivelli AR, Puschenreiter M, De Maria S (2014) Assessment of cadmium uptake and nutrient content in sunflower plants grown under Cd stress. Plant Soil Environ 60(2):80–86. https://doi.org/10.17221/520/2013-PSE
Roy M, McDonald LM (2015) Metal uptake in plants and health risk assessments in metal-contaminated smelter soils. Land Degrad Dev 26(8):785–792. https://doi.org/10.1002/ldr.2237
Rylott EL, Bruce NC (2022) Plants to mine metals and remediate land: Engineered plants can clean up pollution and recover technology-critical metals. Science 377(6613):1380–1381. https://doi.org/10.1126/science.abn6337
Sairam RV, Prakash CS (2005) Can agricultural biotechnology contributes to global food security. In Vitro Cell Dev Biol Plant 41:424–430. https://doi.org/10.1079/IVP2005663
Saleem M, Asghar HN, Khan MY, Zahir ZA (2015) Gibberellic acid in combination with pressmud enhances the growth of sunflower and stabilizes chromium (VI)-contaminated soil. Environ Sci Pollut Res 22(14):10610–10617. https://doi.org/10.1007/s11356-015-4275-3
Sarma H (2011) Metal hyperaccumulation in plants: a review focusing on phytoremediation technology. J Environ Sci Technol 4(2):118–138
SAS, I. (2002). SAS software. SAS Institute Inc 9, NC, USA.
Seregin IV, Kozhevnikova AD (2006) Physiological role of nickel and its toxic effects on higher plants. Russ J Plant Physiol 53(2):257–277. https://doi.org/10.1134/S1021443706020178
Sinha VB, Grover A, Singh S, Pande V, Ahmed Z (2014) Overexpression of Ran gene from Lepidium latifolium L. (LlaRan) renders transgenic tobacco plants hypersensitive to cold stress. Mol Biol Rep 41(9):5989–5996. https://doi.org/10.1007/s11033-014-3476-z
Sinha VB, Grover A, Yadav PV, Pande V (2018) Salt and osmotic stress response of tobacco plants overexpressing Lepidium latifolium L. Ran GTPase gene. Ind J Plant Physiol 23(3):494–498. https://doi.org/10.1007/s40502-018-0396-2
Sofo A, Scopa A, Nuzzaci M, Vitti A (2015) Ascorbate peroxidase and catalase activities and their genetic regulation in plants subjected to drought and salinity stresses. Int J Mol Sci 16(6):13561–13578. https://doi.org/10.3390/ijms160613561
Umar, S., Moinuddin, I. M., & Iqbal, M. (2005). Heavy metal: availability, accumulation and toxicity in plants. Physiology of abiotic stress in plants. Agrobios (India), Jodhpur, 325–348.
Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. Plant Sci 151(1):59–66. https://doi.org/10.1016/S0168-9452(99)00197-1
Vincevica-Gaile Z, Klavins M (2012) Transfer of metals in food chain: An example with copper and lettuce. Environ Clim Technol 10(1):21–24
Wang YC, Lee CM, Lee LC, Tung LC, Hsieh-Li HM, Lee-Chen GJ, Su MT (2011) Mitochondrial dysfunction and oxidative stress contribute to the pathogenesis of spinocerebellar ataxia type 12 (SCA12). J Biol Chem 286(24):21742–21754
Wondimu T, Alamerew S, Ayana A, Garedew W (2014) Variablitiy and association of quantitative traits in Achote (Coccinia abyssinica (Lam) Cogn) in Ethiopia. Int J Plant Breed Genet 8(1):1–12
Yabe J, Ishizuka M, Umemura T (2010) Current levels of heavy metal pollution in Africa. J Vet Med Sci 72(10):1257–1263. https://doi.org/10.1292/jvms.10-0058
Yunus M, Iqbal M (1996) Plant Response to Air Pollution. John Wiley & Sons, Chichester, U.K.
Júnior CAL, Oliveira SR, Mazzafera P, Arruda MAZ (2016) Expanding the information about the influence of cadmium on the metabolism of sunflowers: Evaluation of total, bioavailable, and bioaccessible content and metallobiomolecules in sunflower seeds. Environ Exp Bot 125:87–97
Department of Biotechnology, Faculty of Natural and Computational Science, Woldia University, Woldia, Ethiopia