Pérez-Rodríguez Juan Luis, Ramos Aquino Rocio Grechen, Lorente González Gustavo Yasser, González-Olmedo Justo Lorenzo, Martínez Montero Marcos Edel
Keywords:
Harvest maturity, Seed vigour, Seed quality, ROS, Tobacco
Maturity of the seeds can affect their potential longevity. It has been shown that components related to seed quality develop after the moment when the seed has reached its maximum dry mass, known as harvest maturity. In this state seeds have already developed critical adaptation mechanisms, such as detoxification of reactive oxygen species (ROS), which allow it to survive accelerated water loss. The objective of the present study was to determine the effect of maturity on physiological and biochemical indicators associated with the germination and vigour of tobacco seed. Tobacco seeds were collected at 14, 21, 28, 35, 42, 49 and 56 days after anthesis, the moisture content, dry and fresh mass of 1000 seeds and germination power were determined and the vigour of the seeds was evaluated through accelerated aging test and electrolytes leakage. In addition, generation rate of O2·− and H2O2 content were determined, as well as enzyme activity Superoxide Dismutase and Catalase. Tobacco seeds collected at 35 DAA showed superior vigor as a result of greater tolerance to accelerated aging conditions and cell membrane integrity, reflected in decreased electrolyte leakage values, as a consequence of an adequate oxidative balance. The results showed that there is a close relationship between antioxidant enzyme activity, especially CAT activity, and seed development. CAT enzyme activity levels suggest that hydrogen peroxide scavenging may be crucial to avoid oxidative stress on the way to the acquisition of desiccation tolerance in tobacco seeds.
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