Vegetos

Abstract

Nine seed storage proteins of foxtail millet have been chosen mainly to study their physico-chemical properties, primary and secondary structures using computational tools and servers. Primary structure analysis revealed that most of the seed storage proteins under study are balanced in terms of amino acids composition and four of them contain disulphide linkages. Physico-chemical characterization gave a good idea about the properties like isoelectric point (pI), extinction coefficients (EC), instability index (II), aliphatic index (AI) and Grand average hydropathy (GRAVY) that are essential and vital in providing data about the proteins and their properties. Secondary structure analysis predicted that all of them contain mixed secondary structure of random coils, extended sheets, alpha helices and beta turns. Since, secondary structure prediction remains an important step in full tertiary structure prediction; findings of the present study can provide new insight in foxtail millet seed storage protein characterization and their nutritional utilization as grain.