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Upadhyaya Manju Lata, Bahukhandi Amit, Tiwari Deep C., Singh Prakash
Keywords: n Ginkgo biloban , IBA hormone, Clonal propagation, Himalaya
Ginkgo biloba, a living fossil that is endangered and economically important, has been utilized in both traditional and modern medicine systems. The species reported a very poor regeneration pattern and a slow growth rate, therefore, the present aim of the study is to standardize the clonal propagation protocol using different hormones and stem cutting collected from Uttarakhand. Different hormonal concentrations i.e., Indole Butyric Acid (IBA), Bavistin and Potassium Permanganate (KMnO4) alone (100, 200, 300, 400 and 500 ppm) and in combinations (IBA?+?Bavistin, IBA?+?KMnO4, IBA?+?Bavistin?+?KMnO4) in different concentrations (200, 400, 600, 800, and 1000 ppm) were used. As a result, IBA 200 ppm and Bavistin 200 ppm were shown to significantly improve (p?0.05) the best leaf sprouting percentage (61%) after six months. In addition, the sprouting percentages were found to be significantly higher (70%, 69.66%, and 60.00%, respectively), with other combinations of IBA?+?KMnO4?+?Bavistin and IBA?+?Bavistin (600 ppm). The treatment of IBA (300 ppm) led to the highest survival rates (70%) with a maximum (average) number of roots (3.0 ind.) and root length (8.7 cm), while the control had a 5% survival rate. The standardization of clonal propagation technique with different growth hormones and their combinations offers an alternative strategy for its rapid mass multiplication and can be applicable for the conservation of this endangered species in future.
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Department of Botany, Soban Singh Jeena University, Almora, India