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Keywords: Medicinal plants, MCF-7, n Sesbania grandifloran , Anticancer, Chemical profiling
Sesbania grandiflora, a traditional herb within the Fabaceae family, has been historically employed in traditional therapeutic practices to address various health conditions. Recent studies highlight the effectiveness of this flowering plant as a cytotoxic agent against cancer cells. However, a comprehensive chemical profiling of this plant species still requires more in-depth study and investigation. This study investigates the anticancer and antimicrobial potential of a crystallized neutral lipid fraction (20% EA) obtained from Sesbania grandiflora flowers and its potential chemical components. Chemical characterization of the crystalized fraction through mass spectroscopy and infrared analysis revealed the presence of n-hexadecanoic acid, 1-octadecene, Oleic Acid, and Octadecanoic acid. Investigation reveals a dose-dependent anti-cancer activity against MCF-7 breast cancer cell lines. In vitro bioactivity studies indicated a high IC50 value of 105.76 µg/mL against the MCF-7 breast cancer cell line, with microscopic evidence of dose-dependent cell death. Further research is recommended to crystallize each compound individually and explore potential synergistic effects for therapeutic applications.
Al Habsi AAS, Hossain MA (2018) Isolation, structure characterization and prediction of antioxidant activity of two new compounds from the leaves of Dodonaea viscosa native to the Sultanate of Oman. Egypt J Basic Appl Sci 5:157–164. https://doi.org/10.1016/j.ejbas.2018.04.004
Amaral S, Mira L, Nogueira JMF, Silva AP, da, Helena Florêncio M (2009) Plant extracts with anti-inflammatory properties-A new approach for characterization of their bioactive compounds and establishment of structure-antioxidant activity relationships. Bioorg Med Chem 17:1876–1883. https://doi.org/10.1016/j.bmc.2009.01.045
Aparna V, Dileep KV, Mandal PK, Karthe P, Sadasivan C, Haridas M (2012) Anti-inflammatory property of n-Hexadecanoic acid: structural evidence and kinetic Assessment. Chem Biol Drug Des 80:434–439. https://doi.org/10.1111/j.1747-0285.2012.01418.x
Arthanari SK, Vanitha J, Ganesh M, Venkateshwaran K, Clercq D (2012) Evaluation of antiviral and cytotoxic activities of methanolic extract of S. grandiflora (Fabaceae) flowers. Asian Pac J Trop Biomed 2:S855S858. https://doi.org/10.1016/S2221-1691(12)60323-2
Belakhdar G, Benjouad A, Abdennebi EH (2015) Determination of some bioactive chemical constituents from Thesium Humile Vahl. J Mater Environ Sci 6:2778–2783
Sunil A, Mukunda A, Pynadath A, Mohan MK, Aswathy A (2020) Anti-cancer potency of garlic (Allium sativum) extract in comparison to 5-fluorouracil - an in vitro study. Oral Maxillofac Pathol J 11:11–15
China R, Mukherjee S, Sen S, Bose S, Datta S, Koley H, Ghosh S, Dhar P (2012) Antimicrobial activity of Sesbania grandiflora flower polyphenol extracts on some pathogenic bacteria and growth stimulatory effect on the probiotic organism Lactobacillus acidophilus. Microbiol Res 167:500–506. https://doi.org/10.1016/j.micres.2012.04.003
Contreras-Padilla M, Rivera-Muñoz EM, Gutiérrez-Cortez E, del López AR, Rodríguez-García ME (2015) Characterization of crystalline structures in Opuntia ficus-indica. J Biol Phys 41:99–112. https://doi.org/10.1007/s10867-014-9368-6
Deepthi K, Renjith PK, Habeeb Rahman K, Chandramohanakumar N (2023a) A comprehensive review of Sesbania grandiflora (L.) Pers: traditional uses, phytochemistry and pharmacological properties. https://doi.org/10.1007/s42535-023-00594-5. Vegetos
Deepthi K, Renjith PK, Shameem K, Habeeb Rahman K, Chandramohanakumar N (2023b) Phytochemical screening of leaves and flower extracts of Sesbania grandiflora (L.) Pers. and its antimicrobial activity against fish pathogens. Vegetos 36:626–633. https://doi.org/10.1007/s42535-022-00448-6
Derenne A, Van Hemelryck V, Lamoral-Theys D, Kiss R, Goormaghtigh E (2013) FTIR spectroscopy: a new valuable tool to classify the effects of polyphenolic compounds on cancer cells. Biochim Biophys Acta Mol Basis Dis 1832:46–56. https://doi.org/10.1016/j.bbadis.2012.10.010
Dilika F, Bremner PD, Meyer JJM (2000) Antibacterial activity of linoleic and oleic acids isolated from Helichrysum pedunculatum: a plant used during circumcision rites. Fitoterapia 71:450–452. https://doi.org/10.1016/S0367-326X(00)00150-7
Gandhi AD, Vizhi DK, Lavanya K, Kalpana VN, Rajeswari D, Babujanarthanam V, R (2017a) In vitro anti- biofilm and anti-bacterial activity of Sesbania grandiflora extract against Staphylococcus aureus. Biochem Biophys Rep 12:193–197. https://doi.org/10.1016/j.bbrep.2017.10.004
Gandhi AD, Vizhi DK, Lavanya K, Kalpana VN, Rajeswari D, Babujanarthanam V, R (2017b) In vitro anti- biofilm and anti-bacterial activity of Sesbania grandiflora extract against Staphylococcus aureus. Biochem Biophys Rep 12:193–197. https://doi.org/10.1016/j.bbrep.2017.10.004
Ganesan T, Subban M, Leslee C, Kuppannan DB, Seedevi SB, P (2022) Structural characterization of n-hexadecanoic acid from the leaves of Ipomoea eriocarpa and its antioxidant and antibacterial activities. Biomass Convers Biorefinery. https://doi.org/10.1007/s13399-022-03576-w
Ganesh M, Mohankumar M (2017) Extraction and identification of bioactive components in Sida cordata (Burm.f.) Using gas chromatography–mass spectrometry. J Food Sci Technol 54:3082–3091. https://doi.org/10.1007/s13197-017-2744-z
Harvey HR (1994) Fatty acids and sterols as source markers of organic matter in sediments of the North Carolina continental slope. Deep Res Part II 41:783–796. https://doi.org/10.1016/0967-0645(94)90048-5
Jain P, Soni A, Jain P, Bhawsar J (2016) Phytochemical analysis of Mentha spicata plant extract using UV-VIS, FTIR and GC/MS technique. J Chem Pharm Res 8(2): 1–6
Jerard C, Michael BP, Chenicheri S, Vijayakumar N, Ramachandran R (2020) Rosmarinic Acid-Rich Fraction from Mentha arvensis synchronizes Bcl/Bax expression and induces Go/G1 arrest in Hepatocarcinoma Cells. Proc Natl Acad Sci India Sect B Biol Sci 90:515–522. https://doi.org/10.1007/s40011-019-01122-9
Jiang L, Wang W, He Q, Wu Y, Lu Z, Sun J, Liu Z, Shao Y, Wang A (2017) Oleic acid induces apoptosis and autophagy in the treatment of Tongue squamous cell carcinomas. Sci Rep 7:1–11. https://doi.org/10.1038/s41598-017-11842-5
Kachlicki P, Piasecka A, Stobiecki M, Marczak Ł (2016) Structural characterization of flavonoid glycoconjugates and their derivatives with mass spectrometric techniques. Molecules 21:1–21. https://doi.org/10.3390/molecules21111494
Kale I, Khan MA, Irfan Y, Veerana GA (2012) Hepatoprotective potential of ethanolic and aqueous extract of flowers of Sesbania grandiflora (Linn) induced by CCl4. Asian Pac J Trop Biomed 2:S670–S679. https://doi.org/10.1016/S2221-1691(12)60294-9
Khaled JM, Alyahya SA, Chenthis Kanisha C, Alharbi NS, Kadaikunnan S, Ramachandran G, Alanzi KF, Rajivgandhi G, Vimala RTV, Manoharan N (2021) Anti-biofilm activity of LC-MS based Solanum nigrum essential oils against multi drug resistant biofilm forming P. Mirabilis. Saudi J Biol Sci 28:302–309. https://doi.org/10.1016/j.sjbs.2020.10.003
Kim JS, Kim DK, Moon JY, Lee MY, Cho SK (2024) Oleic acid inhibits the migration and invasion of breast cancer cells with stemness characteristics through oxidative stress-mediated attenuation of the FAK/AKT/NF-κB pathway. J Funct Foods 116:106224. https://doi.org/10.1016/j.jff.2024.106224
Laladhas KP, Cheriyan VT, Puliappadamba VT, Bava SV, Unnithan RG, Vijayammal PL, Anto RJ (2010) A novel protein fraction from Sesbania grandiflora shows potential anticancer and chemopreventive efficacy, in vitro and in vivo. J Cell Mol Med 14:636–646. https://doi.org/10.1111/j.1582-4934.2008.00648.x
Mahmood A, Ahmed R, Kosar S (2009) Phytochemical screening and biological activities of the oil components of Prunus domestica Linn. J Saudi Chem Soc 13:273–277. https://doi.org/10.1016/j.jscs.2009.10.008
Mir FA, Bhat GM, Asokan K, Batoo KM, Banday JA (2014) Crystal structure, morphological, optical and electrical investigations of oxypeucedanin micro crystals: an isolated compound from a plant. J Mater Sci Mater Electron 25:431–437. https://doi.org/10.1007/s10854-013-1606-3
Mostafa AA, Al-Askar AA, Almaary KS, Dawoud TM, Sholkamy EN, Bakri MM (2018) Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi J Biol Sci 25:361–366. https://doi.org/10.1016/j.sjbs.2017.02.004
Murukesh N (2014) Bioactive metabolites and biomarkers from rhizophoraceae mangroves- a chemotaxonomic approach
Noviany N, Nurhidayat A, Hadi S, Suhartati T, Aziz M, Purwitasari N, Subasman I (2018) Sesbagrandiflorain A and B: isolation of two new 2-arylbenzofurans from the stem bark of Sesbania grandiflora. Nat Prod Res 32:2558–2564. https://doi.org/10.1080/14786419.2018.1425858
Noviany N, Samadi A, Yuliyan N, Hadi S, Aziz M, Purwitasari N, Mohamad S, Ismail NN, Gable KP, Mahmud T (2020) Structure characterization and biological activity of 2-arylbenzofurans from an Indonesian plant, Sesbania grandiflora (L. Pers Phytochem Lett 35:211–215. https://doi.org/10.1016/j.phytol.2019.12.008
Pu Zhui, Zhang Y, qun, Yin Z, qiong, Xu J, Jia Ryong, Lu Y, Yang F (2010) Antibacterial activity of 9-octadecanoic acid-hexadecanoic acid-tetrahydrofuran-3,4-diyl ester from neem oil. Agric Sci China 9:1236–1240. https://doi.org/10.1016/S1671-2927(09)60212-1
Ruban P, Gajalakshmi K (2012) In vitro antibacterial activity of Hibiscus rosa-sinensis flower extract against human pathogens. Asian Pac J Trop Biomed 2:399–403. https://doi.org/10.1016/S2221-1691(12)60064-1
Saifudin A, Forentin AM, Fadhilah A, Tirtodiharjo K, Melani WD, Widyasari D, Saroso TA (2016) Bioprospecting for anti-Streptococcus mutans: the activity of 10% Sesbania grandiflora flower extract comparable to erythromycin. Asian Pac J Trop Biomed 6:751–754. https://doi.org/10.1016/j.apjtb.2016.07.004
Sakka Rouis-Soussi L, Ayeb-Zakhama E, Mahjoub A, Flamini A, Jannet GB, Harzallah-Skhiri H, F (2014) Chemical composition and antibacterial activity of essential oils from the Tunisian Allium Nigrum L. EXCLI J 13:526–535
Selvaraju R, Sakuntala P, Jaleeli KA (2021) GC–MS and FTIR Analysis of Chemical compounds in Ocimum Gratissimum Plant. Biophys (Russian Fed 66:401–408. https://doi.org/10.1134/S0006350921030167
Thissera B, Visvanathan R, Khanfar MA, Qader MM, Hassan MHA, Hassan HM, Bawazeer M, Behery FA, Yaseen M, Liyanage R, Abdelmohsen UR, Rateb ME (2020) Sesbania grandiflora L. Poir leaves: a dietary supplement to alleviate type 2 diabetes through metabolic enzymes inhibition. South Afr J Bot 130:282–299. https://doi.org/10.1016/j.sajb.2020.01.011
Inter University Centre for Development of Marine Biotechnology, Cochin University of Science and Technology, Cochin, India