Study of biochemical and biophysical adjustments during transition from desiccation-to-fully-hydrated states in Riccia gangetica and Semibarbula orientalis

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Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00409-z
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Keywords: Abiotic stress, Desiccation, Bryophytes, Semibarbula orientalis , Riccia gangetica , Antioxidants, Photosynthesis, Chlorophyll a fluorescence, Reactive oxygen species, Hypo-osmotic shock


Abstract


Liverworts are considered as the earliest-divergent clade of land plants, while in phylogenetics mosses are the more advanced sister group to a clade that existed between hornworts and tracheophytes. In the present studies, a comparative analysis was done to understand the biochemical and physiological changes during transition from desiccated to hydrated phases of Semibarbula orientalis (moss) and Riccia gangetica (Liverwort). In dehydrated state, resurrection plants enter in quiescent phase and stop all molecular, biochemical and physiological processes until the following rehydration. Biochemical (chl content, proline accumulation, expression of antioxidant enzymes) and biophysical (fluorescence values, density of active reaction centers, specific and phenomenological fluxes, and performance of PSII) studies were done to unravel the mechanism of reactivation during the transition from desiccation-to-hydration. In both bryophytes, SOD, CAT and POD remained in inactive form in the fully desiccated state. In contrast, upon the availability of water, rapid upregulation in the activity of antioxidant enzymes was observed in S. oreintalis as compared with R. gangetica. Even upto 15 min of rehydration, significant accumulation of proline reflects its role to prevent hypo-osmotic shock in S. oreintalis. Rehydration quickly activated photosynthetic machinery in S. oreintalis than R. gangetica. The results clearly reflect that S. oreintalis have more efficient survival strategies at biochemical and physiological levels as compared with R. gangetica.

Abiotic stress, Desiccation, Bryophytes, 
              Semibarbula orientalis
            , 
              Riccia gangetica
            , Antioxidants, Photosynthesis, Chlorophyll a fluorescence, Reactive oxygen species, Hypo-osmotic shock


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Acknowledgements



Author Information


Bhatt Upma
Plant Bioenergetics and Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur, India

Soni Vineet
Plant Bioenergetics and Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur, India
vineetsonijnu@gmail.com