Sustainable management of Meloidogyne incognita infecting carrot (Daucus carota): Green synthesis of silver nanoparticles with Cnidoscolus aconitifolius

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-021-00216-y
First Page: 277
Last Page: 285
Views: 772

Keywords: Environmental pollution, Nematicide, Meloidogyne incognita , Synthetics, Carbofuran


Pests bring about significant decrease in carrot production which often cause to huge financial loss. A major constraint facing carrot production worldwide is infestation with root knot nematode Meloidogyne incognita. Different management strategies have been utilised in the control of M. incognita with various degrees of favourable outcome. Synthetic nematicides, though globally perceived as the most effective is largely negated by their toxicity and adverse effects on humans and the environment. A sizeable recognition has been redirected to the application of nanoparticles in agriculture. In order to achieve progress in the burgeoning of environmentally safe bio-nematicide, the green synthesis of silver nanoparticles with Cnidoscolus aconitifolius extracts was evaluated on carrot plants inoculated with M. incognita juveniles on the field. Extract from C. aconitifolius was taken as the reducing agent, while silver nitrate is the metal precursor. Colour change by the extracts confirms the formation of AgNP. The AgNPs produced were characterised with UV–Vis absorption spectroscopy, FTIR and SEM. Results revealed the formation of AgNPs with surface plasmon resonance absorption at 410, 423 and 428 nm, SEM confirmed the formation of AgNP as mainly spherical ranging between 2 and 20 nm. AgNPs were applied at 25, 50 and 75 ml which is equivalent to 50, 100 and 150 mol, respectively on the field. AgNPs treated carrots plants had significantly (p < 0.05) higher vegetative growth, with corresponding higher yield contrary to the untreated carrots plants. Plant mediated AgNPs is a promising method of M. incognita control in carrot production.

Environmental pollution, Nematicide, 
                Meloidogyne incognita
              , Synthetics, Carbofuran

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  1. Adeniran OI, Olajide OO, Igwemmar NC, Orishadipe AT (2013) Phytochemical constituents antimicrobial and antioxidant potential of tree spinach [Cnidoscolus aconitifolius (Miller) I.M Johnston]. J Med Plant Res 7(19):1317–1322

  2. Amaladhas S, Sivagami TP, Devi TA, Ananthi N, Velammal SP (2012) Biogenic synthesis of silver nano particles by leaf extract of Cassia angustifolia. Adv Nat Sci Nano Sci Nano Technol 3:045–048

  3. Arscot SA, Tanumihardio SA (2010) Carrots of many colours provide basic nutrition and bio-available phytochemicals acting as a functional food. Com Rev Food Sci and Food Safety 9(2):223–239.

  4. Benjamin LR, McGarry A, Gray D (1997) The root vegetables: beet, carrot, parsnip and turnip. The physiology of vegetable crops. CAB International, Wallingford, UK. pp 553–580. ISBN 978-0-85199-146-7

  5. Bontempo AF, Lopes EA, Fernandes RH, de Grassi FL, Dallemole-Giaretta R (2017) Dose-response effect of Pochonia chlamydosporia against Meloidogyne incognita on carrot under field conditions. Revista Caatinga 30(1):129.

  6. Carlos J, Dias S (2014) Nutritional and health benefits of carrots and their seed extracts. Food Nutr Sci 5:2147–2156.

  7. Coyne DL, Hendrika FH, Moens M (2009) Current and future management strategies in resource-poor farming. In: Perry RN, Moens M, Starr JL (eds) Root knot nematodes. CAB International, Wallingford, pp 444–475

  8. Davis RM (2004) Carrot diseases and their management. In: Naqvi SAMH (ed) Diseases of fruits and vegetables: diagnosis and management. Springer, pp 397–439. ISBN 978-1-4020-1822-0

  9. Devatha CP, Jagadeesh K, Mallikarjun P (2018) Effect of Green synthesized iron nanoparticles by Azardirachta Indica in different proportions on antibacterial activity. Env Nanotechnol Monitor Manage 9:84–94

  10. Essing FB (2013) What’s in a family? The apiaceae. Florida Garden 18:36–37

  11. Fabiyi OA (2019) Management of groundnut (Arachis hypogea) root-knot nematode (Meloidogyne incognita): effect of Prosopis africana pods. Indian J Nematol 49(2):214–216

  12. Fabiyi OA (2020) Growth and yield response of groundnut Arachis hypogaea (Linn.) under Meloidogyne incognita infection to furfural synthesised from agro-cellulosic materials. J Trop Agric 58(2):241–245

  13. Fabiyi OA (2021) Evaluation of plant materials as root-knot nematode (Meloidogyne incognita) suppressant in okro (Abelmuscous esculentus). Agric Conspec Sci 86(1):51–56

  14. Fabiyi OA, Olatunji GA, Saadu AO (2018) Suppression of Heterodera sacchari in rice with agricultural waste-silver nano particles. J Solid Waste Technol Manage 44(2):87–91

  15. Fabiyi OA, Olatunji GA, Atolani O, Olawuyi RO (2020c) Preparation of bio-nematicidal nanoparticles of Eucalyptus officinalis for the control of cyst nematode (Heterodera sacchari). J Anim Plant Sci 30(5):1172–1177

  16. Fabiyi OA, Atolani O, Olatunji GA (2020a) Toxicity Effect of Eucalyptus globulus to Pratylenchus spp of Zea mays. Sarhad J Agric 36(4):1244–1253

  17. Fabiyi OA, Alabi RO, Ansari RA (2020b) Nanoparticles’ synthesis and their application in the management of phytonematodes: an overview. In: Ansari RA, Rizvi R, Mahmood I (eds) Management of phytonematodes: recent advances and future challenges. Singapore, pp 125–140

  18. González-Laredo RF, Flores ME, Quintero-Ramos MJ, Karchesy JJ (2003) Flavonoid and cyanogenic contents of chaya (Spinach Tree). Plant Foods Hum Nutr 58:1–8

  19. Hay FS, Pethybridge SJ (2005) Nematodes associated with carrot production in Tasmania, Australia, and the effect of Pratylenchus crenatus on yield and quality of Kuroda-type carrot. Plant Dis 89:1175–1180

  20. Hedrén E, Diaz V, Svanburg U (2002) Estimation of carotenoid accessibility from carrots determined by an in vitro digestion method. Eur J Clin Nutr 56(5):425–430.

  21. Iorizzo M, Senalik DA, Ellison SL, Grzebelus D, Cavagnaro PF, Allender C, Brunet J, Spooner DM, Van Deynze A, Simon PW (2013) Genetic structure and domestication of carrot (Daucus carota subsp. sativus) (Apiaceae). Am J Bot 100(5):930–938.

  22. Jansen PCM (2004) Cnidoscolus aconitifolius (Mill) I. M. Johnst. [Internet] Record from PROTA4U. In: Grubben GJH, Denton OA (eds) PROTA (Plant Resources of Tropical Africa/Ressources végétales de l’Afrique tropicale), Wageningen, Netherlands. Accessed 10 Oct 2018

  23. Kalaiselvi D, Mohankumar A, Shanmugam G, Nivitha S, Sundararaj P (2019) Green synthesis of silver nanoparticles using latex extract of Euphorbia tirucalli: a novel approach for the management of root knot nematode Meloidogyne incognita. Crop Prot 117:108–114

  24. Khan MR, Rizvi TF (2014) Nanotechnology: scope and application in plant disease management. Plant Pathol J 13:214–231

  25. Khodashenas B, Ghorbani HR (2015) Synthesis of silver nano particles with different shapes. Arab J Chem 12(14):132–143.

  26. Kouvaris P, Delimitis A, Zaspalis V, Papadopoulus D, Tsipas S, Michailidis N (2012) Green synthesis and characterisation of silver nano particles produced using Arbutus unedo leaf extract. Mater Lett 76:18–20

  27. Kuti JO, Konoru HB (2006) Cyanogenic glycosides content in two edible leaves of tree spinach (Cnidoscolus spp). J Food Comp Anal 19(6–7):556–561

  28. Maggie EMH, Hanaa S, Zawam SE, El-Nahas M, Abeer FD (2016) Comparison study between silver nanoparticles and two Nematicides against Meloidogyne incognitaon tomato seedlings. Plant Pathol J 15:144–151

  29. Makarov VV, Love AJ, Sinitsyna OV, Makarova SS, Yaminsky IV, Taliansky ME, Kalinina NO (2014) Green nanotechnologies: synthesis of metal nanoparticles using plants. Acta Naturae 6(1):30–44

  30. Markus V, Paul AA, Yahaya J, Zakka J, Yatai KB, Oladeji M (2016) An underexploited tropical plant with promising economic value and the window of opportunities for researchers: Cnidoscolus aconitifolius. Am J Food Sci Nutr Res 3(6):177–187

  31. Megueni C, Tchuenteu TL, Noubissie E, Maimouna A, Bachirou H (2017) Field productivity of carrot (Daucus carota L.) in Adamawa Cameroon and chemical properties of roots according to chicken manure pre-treatments and vivianite powder. J Agric Vet Sci 10(5):16–23

  32. Noling JW (2016) Nematode management in carrots. ENY-021.

  33. Fabiyi OA, Olatunji GA (2018) Application of green synthesis in nano particles preparation: Ficus mucoso extracts in the management of Meloidogyne incognita parasitizing groundnut Arachis hypogea. Indian J Nematol 48(1):13–17

  34. Oyagbemi AA, Odetola AA, Azeez OI (2011) Phytochemical investigation and proximate analysis on the leaves of Cnidoscolus aconitifolius. J Med Food 14(3):322–324.

  35. Perry RN, Moens M (2011) Introduction to plant parasitic nematodes; modes of parasitism. In: Jones J, Gheysen G, Fenoll C (eds) Genomics and molecular genetics of plant-nematode interactions. Springer, Netherlands, pp 3–20

  36. Poovathinthodiyil R, Jie F, Scott LW (2003) Completely “green” synthesis and stabilization of metal nanoparticles. J Am Chem Soc 125:13940–13941

  37. Rajput VD, Tatiana M, Minkina AB, Svetlana N, Sushkova SM, Ritu S, Andrey G, Viktoriia S, Tsitsuashvili WO, Purvis KA, Ghazaryan HSM (2017) Effects of zinc-oxide nano particles on soil, plants, animals and soil organisms: a review. Env Nanotechnol Monitor Manage 9:76–84

  38. Ranhotra GS, Gelroth JA, Leinen SD, Viñas MA, Lorenz K (1998) Nutritional profile in some edible plants from Mexico. J Food Compos Anal 2:298–304

  39. Renco M, Kovacik P (2012) Response of plant parasitic and free living soil nematodes to composted animal manure soil amendments. J Nematol 44(4):329–336

  40. Satish K, Nune NC, Ravi S, Kavita K, Rajesh R, Kulkarni ST, Swapna M, Raghuraman K, Kattesh VK (2009) Green nanotechnology from tea: phytochemicals in tea as building blocks for production of biocompatible gold nanoparticles. J Mater Chem 19(19):2912–2920

  41. Shankar SS, Rai A, Ankamwar B, Singh A, Ahmad A, Sastry M (2004) Biological synthesis of triangular gold nanoprisms. Nat Mater 3:482–488.

  42. Sharma KD, Karki S, Thakur NS, Attri S (2012) Chemical composition, functional properties and processing of carrot-a review. J Food Sci Technol 49(1):22–32.

  43. Siddiqui ZA, Parveen A, Lukman AL, Hashem A (2019) Effects of graphene oxide and zinc oxide nanoparticles on growth, chlorophyll, carotenoids, proline contents and diseases of carrot. Sci Hortic 249:374–382

  44. Sikora RA, Fernandez E (2005) Nematode parasites of vegetables. In: Luc M, Sikora RA, Bridge J (eds) Plant parasitic nematodes in subtropical and tropical agriculture. CAB International, Wallingford, pp 329–330

  45. Simon PW, Freeman REV, Jairo V (2008) "Carrot". Vegetables II. Handbook of Plant Breeding, vol 2. Springer, New York, pp 327–357. ISBN 978-0-387-74108-6

  46. Sondi I, Salopek-Sondi B (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for gram-negative bacteria. J Colloid Interface Sci 275:177–182

  47. Song JY, Jang HK, Kim BS (2009) Biological synthesis of gold nanoparticles using Magnolia kobus and Diopyros kaki leaf extracts. Process Biochem 44:1133

  48. Umuhoza JN, Karemera HS, Sibomana P (2014) Nutritional quality of carrot (Daucas carota L) as influenced by farm yard manure. World Sci Res J 2(5):102–107


  50. Vijayakumar MK, Priya FT, Nancy A, Noorlidah A, Ahmed ABA (2013) Biosynthesis, characterisation and anti-bacterial effect of plant-mediated silver nanoparticles using Artemisia nilagirica. Ind Crops Prod 41:235–240

  51. Vrain TC (1981) Relationship between Meloidogyne hapla density and damage to carrots in organic soils. J Nematol 14:50–57

  52. Wagini NH, Abubakar S (2015) Incidence and severity of common diseases of carrot and their pathogenic agents in northern Nigeria. Katsina J Nat Appl Sci 4:212–219

  53. Whitehead AG, Hemming JR (1965) A comparison of some qualitative methods of extracting small vermiform nematodes from soil. Ann Appl Biol 55:25–38

  54. Yadav RN, Chitara MK, Zaidi NW, Khan AI, Singh US, Singh HB (2018) Novel facets and challenges in the management of phytopathogens using myconanoparticles. Int J Curr Microbiol App Sci 7(12):3296–3308

  55. Zhou Y, Wenshuang L, Jiale H, Wenta W, Yixian G, Liqin L, Qingbiao L, Ling L, Mingming D (2010) Biosynthesis of gold nanoparticles by foliar broths: roles of bio compounds and other attributes of the extracts. Nano Scale Res Lett 5:1351–1359



Author Information

Fabiyi Oluwatoyin Adenike
Department of Crop Protection, Faculty of Agriculture, University of Ilorin, Ilorin, Nigeria