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Kaur Jashanpreet, Kaur Kamaldeep, Pathak Shalini, Vyas Ashish
Keywords: n Citrus sinensisn , n Curcuma longan , n Aspergillusn , Antifungal activity, Synergistic effect
This study investigates the antifungal efficacy of turmeric (Curcuma longa) rhizome extract and orange peel (Citrus sinensis) extract, both individually and in combination, against three Aspergillus species: A. fumigatus, A. niger, and A. nidulans. The antifungal activity was assessed using a disc diffusion method, and the results of individual crude extracts were compared with a positive control, Amphotericin-B and a negative control, Dimethyl sulfoxide (DMSO). With the rise of antimicrobial resistance and concerns over the side effects and environmental impact of synthetic antifungal agents, there is an increasing need for natural alternatives. Plant-based extracts, such as those from turmeric and orange peel, offer a sustainable, eco-friendly approach to combating fungal pathogens. This study highlights the potential of these extracts as effective antifungal agents and suggests that their combination could be a promising alternative in controlling fungal infections caused by Aspergillus species.
Abdel-Azeem AM, Abdel-Azeem MA, Abdul-Hadi SY, Darwish AG (2019) Aspergillus: biodiversity, ecological significances, and industrial applications. In: Singh BP (ed) Recent advancement in white biotechnology through fungi: volume 1: diversity and enzymes perspectives. Springer, Cham, pp 121–179. https://doi.org/10.1007/978-3-030-10480-1_4
Azeem K, Irfan I, Shakir M, Rawat DS, Abid M (2024) Therapeutic potential of phytochemicals and their derivatives as antifungal candidates: recent discovery and development. In: Verma A (ed) Advances in antifungal drug development: natural products with antifungal potential. Springer, Singapore, pp 517–534
Barwant M, Lavhate N (2020) Isolation and maintenance of fungal pathogens Aspergillus niger and Aspergillus flavus. Int J Appl Nat Sci 9(3):47–52.
Diniso T, Oriola AO, Adeyemi JO, Miya GM, Hosu YS, Oyedeji OO et al (2024) Citrus wastes: a valuable Raw material for biological applications. J Appl Pharm Sci 14(8):11–26
Eschenauer GA (2024) Antifungal therapies for Aspergillus spp.: present and future. Semin Respir Crit Care Med 45(1):61–68
Gachara G, Suleiman R, Kilima B, Taoussi M, El Kadili S, Fauconnier ML et al (2024) Pre-and post-harvest aflatoxin contamination and management strategies of Aspergillus spoilage in East African community maize: review of etiology and climatic susceptibility. Mycotoxin Res 40(4):495–517
Gilman JC (1957) A manual of soil fungi, 2nd edn. Iowa State College, Ames, Iowa, USA
Hernández A, Ruiz-Moyano S, Galván AI, Merchán AV, Nevado FP, Aranda E et al (2021) Anti-fungal activity of phenolic sweet orange peel extract for controlling fungi responsible for post-harvest fruit decay. Fungal Biol 125(2):143–152
Jha AK, Sit N (2022) Extraction of bioactive compounds from plant materials using combination of various novel methods: a review. Trends Food Sci Technol 119:579–591. https://doi.org/10.1016/j.tifs.2021.11.019
Jikah AN, Edo GI (2025) Turmeric (Curcuma longa): an insight into its food applications, phytochemistry and pharmacological properties. Vegetos 38(3):845–866. https://doi.org/10.1007/s42535-024-01038-4
Ma M, Li A, Feng J, Wang Z, Jia Y, Ma X, Ning Y (2024) Antifungal mechanism of Lactiplantibacillus plantarum P10 against Aspergillus Niger and its in-situ biopreservative application in Chinese steamed bread. Food Chem 449:139181. https://doi.org/10.1016/j.foodchem.2024.139181
Ngwu MI, Ngwu GI, Okorie CN, Emencheta SC, Odimegwu DC, Attama AA, Ibezim EC (2024) Synergistic antifungal activity of combinations of Artemisia annua extract with fluconazole against resistant strains of Candida albicans. Trop J Pharm Res 23:941–949. https://doi.org/10.4314/tjpr.v23i6.4
Samson RA, Visagie CM, Houbraken J, Hong SB, Hubka V, Klaassen CH, Perrone G, Seifert KA, Susca A, Tanney JB, Varga J (2014) Phylogeny, identification and nomenclature of the genus Aspergillus. Stud Mycol 78(1):141–173
Vitiello A, Ferrara F, Boccellino M, Ponzo A, Cimmino C, Comberiati E et al (2023) Antifungal drug resistance: an emergent health threat. Biomedicines 11(4):1063
Zhang D, Yang Y, Yao B, Hu T, Ma Z, Shi W, Ye Y (2023) Curcumin inhibits Aspergillus flavus infection and aflatoxin production possibly by inducing ROS burst. Food Res Int 167:112646
Bongomin F, Gago S, Oladele RO, Denning DW (2017) Global and multi-national prevalence of fungal diseases—estimate precision. J Fungi 3(4):57
Department of Botany, Akal University, Bathinda, India