The effects of foliar application of chitosan on the morphological and chemical characters of French lavender against water deficiency

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Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-023-00631-3
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Keywords: Chitosan, Water deficiency, Growth, Essential oil, Chemical composition


Abstract


French lavender has multiple biological qualities that are attributed to it being a source of natural products that are used for medicinal and food purposes. The rapid water loss in sandy reclamation soils in Egypt negatively affects the productivity of French lavender plants. Thus the aim of this trial was to evaluate the morphological and chemical measurements of French lavender treated with chitosan under various rates of water deficiency. Plants subjected to different rates of soil moisture at 100, 80, 60 and 40% corresponding to field water capacity without or with chitosan at 4 and 6 mg L−1 as foliar spray. Fresh and dry weights of aerial parts, essential oil compositions, photosynthetic pigments, phenols, flavonoids, free radical scavenging activity, proline, carbohydrates, protein and nutrients (nitrogen, phosphorous and potassium) were measured. Obtained results were analyzed according to 2- ways analysis of variance. Plants treated with water deficiency levels and chitosan together gave higher values of morphological characters, photosynthetic pigments, phenols, flavonoids, proline, carbohydrates, protein and nutrients than those exposed to water deficiency treatments. On the other hand, chitosan × water deficiency treatments resulted in lower values of free radical scavenging activity than water deficiency doses. It may be concluded that application of chitosan to French lavender growing in reclaimed soils is considered a way to retain water and improve its productivity, which helps to expand its cultivation in arid regions as a source of natural products that are used in various pharmaceutical and food purposes.



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References


Aalipour H, Nikbakht A, Sabzalian MR (2023) Essential oil composition and total phenolic content in Cupressus arizonica G. in response to microbial inoculation under water stress conditions. Sci Rep 13:1209


Adams RP (1995) Identification of essential oil components by gas chromatography/mass spectrometry. Allured Publ. Corp, Carol Stream


Ahmed AMA, Talaat IM, Khalid AK (2017) Soil Moisture and glutamic acid affect yield, volatile oil and proline contents of oregano herb (Origanum vulgare L.). Int J Botany 13(1):43–51


Ahmed AMA, El-Gohary AE, Osman SA, Khalid KA (2020) Arginine and salinity stress affect morphology and metabolism of Indian borage (Plectranthus amboinicus lour.). Acta Ecol Sin 40(5):417–424


Akhtar G, Faried HN, Razzaq K, Ullah S, Wattoo FM, Shehzad MA, Sajjad Y, Ahsan M, Javed T, Dessoky ES, Abdelsalam NR, Chattha MS (2022) Chitosan-induced physiological and biochemical regulations confer drought tolerance in pot marigold (Calendula officinalis L.). Agronomy 12:474


Ali EF, El-Shehawi AM, Ibrahim OHM, Abdul-Hafeez EY, Moussa MM, Hassan FAS (2021) A vital role of chitosan nanoparticles in improvisation the drought stress tolerance in Catharanthus roseus (L.) through biochemical and gene expression modulation. Plant Physiol Biochemi 161:166–175


Almeida LG, Magalhães PC, Karam D, Silva EMD, Alvarenga AA (2019) Chitosan application in the induction of water deficit tolerance in maize plants. Acta Sci Agron 42:e42463


Anonymous (2016) Official methods of analysis, 20th edn. Association of Official Analytical Chemists, Washington DC


Auerbach BJ, Reynolds SJ, Lamorde M, Merry C, Kukunda-Byobona C, Ocama, (2012) Traditional herbal medicine use associated with liver fibrosis in rural Rakai. Uganda Plos ONE 7:e41737


Ávila RG, Magalhães PC, Vitorino LC, Bessa LA, Souza KRD, Queiroz RB, Jakelaitis A, Teixeira B (2023) Chitosan induces sorghum tolerance to water deficits by positively regulating photosynthesis and the production of primary metabolites, osmoregulators, and antioxidants. J Soil Sci Plant Nutr 23:1156–1172


Baeck H, Kuenwoo P, Baeck HW, Park KW (2001) Effect of watering on growth and oil content of sweet basil (Ocimum americanum L.). Korean J Hortic Sci Technol 19(1):81–86


Baher ZF, Mirza M, Ghorbanil M, Rezaii MZ (2002) The influence of water stress on plant height, herbal and essential oil yield and composition in Satueja hortensis L. Flavour Fragr J 17:275–277


Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline of water stress studies. Plant Soil 39:205–207


Bistgani ZE, Siadat SA, Bakhshandeh A, Pirbalouti AG, Hashemi M (2017) Interactive effects of drought stress and chitosan application on physiological characteristics and essential oil yield of Thymus daenensis Celak. Crop J 5(5):407–415


Burbott AJ, Loomis D (1969) Evidence for metabolic turnover monoterpene in peppermint. Plant Physiol 44:173–179


Chapman HD, Pratt PF (1978) Methods of analysis for soils, plants and water. Division of Agriculture Sciences, University of California, Davis


Clevenger JF (1928) Apparatus for determination of essential oil. J Am Pharm Assoc 17:346–349


Cottenie A, Verloo M, Kiekens L, Velgh G, Camerlynck R (1982) Chemical analysis of plant and soils. Laboratory of analytical and agro chemistry. State University of Gent publishing, Belgium


Dash M, Chiellini F, Ottenbrite R, Chiellini E (2011) Chitosan—a versatile semi synthetic polymer in biomedical applications. Prog Polym Sci 36:981–1014


Dianat M, Saharkhiz MJ, Tavassolian I (2016) Salicylic acid mitigates drought stress in Lippia citriodora L.: effects on biochemical traits and essential oil yield. Biocatal Agric Biotechnol 8:286–293


Dowom AS, Karimian Z, Dehnavi M, Samiei L (2022) Chitosan nanoparticles improve physiological and biochemical responses of Salvia abrotanoides (Kar.) under drought stress. BMC Plant Biol 22:364


Dubois M, Gilles KA, Hamilton JK, Roberts PA, Smith F (1956) Phenol sulphuric acid method for carbohydrate determination. Ann Chem 28:350–359


El-Sherif AF, Shehata SM, Youssif RM (1990) Response of tomato seedlings to zinc application under different salinity levels. Egypt J Hortic Sci 17:131–142


Fahmy AA, Nosir WS (2018) Influence of Chitosan and micronutrients (Fe + Zn) concentrations on growth, yield components and volatile oil of lavender plant. Sci J Flowers Ornam Plants 8(1):87–100


Foucart T (1982) Analyse Factorielle, Programmatiol Sur Micro- ordinateur. Masson ITCF Paris. ISBN-13: 978–2225764509.


Górnik K, Grzesik M, Romanowska DB (2008) The effect of chitosan on rooting of grapevine cuttings and on subsequent plant growth under drought and temperature stress. J Fruit Ornam Plant Res 16:333–343


Gu L (2011) Effects of exogenous chitosan on physiological characteristics of phalaenopsis seedlings under drought stress. South West China J Agric Sci 24:90–93


Hadwiger L, Kendra D, Fristensky B, Wagoner W (1986) Chitosan both activates genes in plants and inhibits RNA synthesis in fungi. In: Chitin in Nature and Technology. Springer, Berlin Germany


Helaly MN, Farouk S, Arafa SA, Amhimmid NBIA (2018) Inducing Salinity Tolerance of rosemary (Rosmarinus officinalis L.) plants by chitosan or zeolite application. Asian J Adv Agric Res. 5(4):1–20


Hong Z, Lakkineni K, Zhang Z, Verma DPS (2000) Removal of feedback inhibition of delta (1)-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress. Plant Physiol 122:1129–1136


Hossain MA, Hoque MA, Burritt DJ, Fujita M (2014) Proline protects plants against abiotic oxidative stress: biochemical and molecular mechanisms. In: Parvaiz A (ed) Oxidative damage to plants antioxidant networks and signaling. Elsevier Inc, New York


Hura T, Hura K, Ostrowska A (2023) Drought stress induced physiological and molecular changes in plants. Int J Mol Sci 24:1773


Ibrahim ME, Mohamed MA, Khalid KA (2014) Effect of growing locations on the essential oil content and compositions of lemon verbena shrubs under the conditions of Egypt. J Essent Oil Bear Plants 17:288–294


Iriti M, Faoro F (2008) Abscisic acid is involved in chitosan-induced resistance to tobacco necrosis virus (TNV). Plant Physiol Biochem 46:1106–1111


Jackson ML (1973) Soil chemical analysis, 1st edn. Prentice Hall Ltd publishing, New Delhi


Jiao Z, Li Y, Li J, Xu X, Li H, Lu D, Wang J (2012) Effects of exogenous chitosan on physiological characteristics of potato seedlings under drought stress and rehydration. Potato Res 55:293–301


Kahromi S, Khara J (2021) Chitosan stimulates secondary metabolite production and nutrient uptake in medicinal plant Dracocephalum kotschy. J Sci Food Agric 101:3898–3907


Khalid KA (2006) Influence of water stress on growth, essential oil, and chemical composition of herbs (Ocimum sp.). Int Agrophys 20:289–296


Khalid AK, Ahmed MA (2017) Growth and certain biochemical components of black cumin cultivated under salinity stress factor. J Mater Environ Sci 8(1):7–13


Khalid AK, da Silva JT, Cai W (2010) Water deficit and polyethylene glycol 6000 affects morphological and biochemical characters of Pelargonium odoratissimum (L.). Sci Hortic 125:159–166


Khalid AK, El-Gohary AE, Ahmed AMA (2020) Effect of growing seasons on the leaf essential oil composition of Citrus species that are cultivated in Egypt. J Essent Oil Rese 32:296–307


Laftouhi A, Eloutassi N, Drioua S, Ech-Chihbi E, Rais Z, Abdellaoui A, Taleb A, Beniken M, Taleb M (2023) Impact of water stress and temperature on metabolites and essential oil of Rosmarinus Officinalis (phytochemical screening, extraction, and gas chromatography). J Ecol Eng 24:237–248


Li Z, Zhang Y, Zhang X, Merewitz E, Peng Y, Ma X, Huang L, Yan Y (2017) Metabolic pathways regulated by chitosan contributing to drought resistance in white clover. J Proteome Res 16:3039–3052


Lim CW, Baek W, Jung J, Kim JH, Lee SC (2015) Function of ABA in stomatal defense against biotic and drought stresses. Int J Mol Sci 16:15251–15270


Malekpoor F, Pirbalout AG, Salimi A (2016) Effect of foliar application of chitosan on morphological and physiological characteristics of basil under reduced irrigation. Res Crops 17(2):354–359


Martínez-Natarén DA, Villalobos-Perera PA, Munguía-Rosas MA (2018) Morphology and density of glandular trichomes of Ocimum campechianum and Ruellia nudiflorain contrasting light environments: a scanning electron microscopy study. Flora 248:28–33


Máthé Á, Khan IA (2022) Introduction to medicinal and aromatic plants in India. Springer, Cham


Middha A, Purohit S (2011) Determination of free radical scavenging activity in herbal supplement: Chyawanprash. Int J Drug Dev Res 3:328–333


Mishra AK, Singh VP (2010) A review of drought concepts. J Hydrol 391:202–216


Misra A, Srivastava NK (2000) Influence of water stress on Japanese mint. J Herbs Spices Med Plants 7(1):51–58


Msaada K, Salem N, Tammar S, Hammami M, Saharkhiz MJ, Debiche N, Limam F, Marzouk B (2012) Essential oil xomposition of Lavanduladentata, L. stoechas and L. multifida cultivated in Tunisia. J Essent Oil Bear Plants. 15(6):1030–1039


Mulugeta SM, Radácsi P (2022) Influence of drought stress on growth and essential oil yield of Ocimum Species. Hortic 8:175


Pirbalouti AG, Malekpoor F, Salimi A, Golparvar A (2017) Exogenous application of chitosan on biochemical and physiological characteristics, phenolic content and antioxidant activity of two species of basil (Ocimum ciliatum and Ocimum basilicum) under reduced irrigation. Sci Hortic 217:114–122


Pongprayoon W, Roytrakul S, Pichayangkura R, Chadchawan S (2013) The role of hydrogen peroxide in Chitosan induced resistance to osmotic stress in rice (Oryza sativa L). Plant Growth Regul 70:159–173


Pourmorad F, Hosseinimehr S, Shahabimajd N (2006) Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotech 5:1142–1145


Rafie S, Namjoyan F, Golfakhrabadi F, Yousefbeyk F, Hassanzadeh A (2016) Effect of lavender essential oil as a prophylactic therapy for migraine: a randomized controlled clinical trial. J Herb Med 6(1):18–23


Reddy T, Reddy V, Anbumozhi V (2003) Physiological responses of groundnut (Arachis hypogea L.) to drought stress and its amelioration: a critical review. Plant Growth Regul 41:75–88


Salehi-Lisar SY, Bakhshayeshan-Agdam H (2016) Drought stress in plants: causes, consequences, and tolerance. In: drought stress tolerance in plants. Springer, Berlin, Germany


Shabala S (2009) Salinity and programmed cell death: unravelling mechanisms for ion specific signaling. J Exp Bot 60:709–711


Sharif R, Mujtaba M, Rahman MU, Shalmani A, Ahmad H, Anwar T, Tianchan D, Wang X (2018) The multifunctional role of chitosan in horticultural crops; a review. Molecules 23:872


Sharma P, Jha A B, Dubey R S, Pessarakli M (2012) Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J Bot. 217037


Simon JE, Reiss BD, Joly RJ, Charles DJ (1992) Water stress induced alternations in essential oil content of sweet basil. J Essent Oil Res 1:71–75


Singh R, Luxmi S, Charak A, Gochar R, Kumar A, Gandhi SG, Bhanwaria R (2022) Effects of intermittent drought on the essential oil yield, contents, and nutrient status of Mentha longifolia (L.) Huds. J Essent Oil Bear Plants 25:626–638


Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin Ciocalteu reagent. Methods Enzymol 299:152–178


Snedecor GW, Cochran WG (1990) Statistical methods, 11th edn. Iowa State University, Press, Ames


Snell R, Snell G (1954) Colorimetric method of analysis. D. Van Nostrand Company, New York


Solinas V, Deiana S (1996) Effect of water and nutritional conditions on the Rosmarinus officinalis L phenolic fraction and essential oil yields. Riv Ital Eposs 19:189–198


Yang F, Hu J, Li J, Wu X, Qian Y (2009) Chitosan enhances leaf membrane stability and antioxidant enzyme activities in apple seedlings under drought stress. Plant Growth Regul 58:131–136




 


Acknowledgements


The authors would like to thank the National Research Centre (NRC) for it’s facilitates during this scientific work.


Author Information


Abd-Rabbu Hala S.
Medicinal and Aromatic Plants Department, National Research Centre, Cairo, Egypt

Wahba Hend E.
Medicinal and Aromatic Plants Department, National Research Centre, Cairo, Egypt


Khalid Khalid A.
Medicinal and Aromatic Plants Department, National Research Centre, Cairo, Egypt

ahmed490@gmail.com