Ultraviolet-B radiation induces physiological, biochemical, and structural changes in Viola odorata

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-020-00139-0
First Page: 545
Last Page: 554
Views: 610

Keywords: Anatomical response, Antioxidant response, Mucilage, Sweet violet, Ultraviolet radiation


Ultraviolet (UV) radiation is a common environmental factor that affects the growth and productivity of plants. However, many plants have evolved structural, physiological, and biochemical mechanisms to cope with UV radiation. Viola odorata, is exposed to extended daily sunlight and acclimatization mechanisms have been developed in response to natural UV radiations. The purpose of this study was to assess the changes in the morphology and abundance of mucilaginous structures besides the physiological and biochemical changes of V. odorata plants under exposure to UV-B radiation (2 h and 4 h daily). After 4 weeks of treatments, the obtained results showed that the V. odorata plants responded to UV-B radiation anatomically and physiologically through changes in thickening of the cuticle, production of mucilage and enzymatic and non-enzymatic antioxidants; also, the content of malondialdehyde (MDA) and hydrogen peroxide (H2O2) was increased. The investigation showed that the duration of exposure to UV-B radiation had a negative correlation with the content of carotenoids, chlorophyll a, chlorophyll b, and soluble carbohydrates, whereas it had a positive correlation with the content of mucilage, anthocyanins, flavonoids, MDA, H2O2, and activity of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POX) enzymes. These findings display the different defense mechanisms in V. odorata plants against UV-B radiation.

Anatomical response, Antioxidant response, Mucilage, Sweet violet, Ultraviolet radiation

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  1. Allen DJ, Nogués S, Baker NR (1998) Ozone depletion and increased UV-B radiation: is there a real threat to photosynthesis? J Exp Bot 49:1775–1788

  2. Arnon DI, Whatley F (1949) Factors influencing oxygen production by illuminated chloroplast fragments. Arch Biochem 23:141

  3. Basu S, Roychoudhury A, Saha PP, Sengupta DN (2010) Differential antioxidative responses of indica rice cultivars to drought stress. Plant Growth Regul 60:51

  4. Berli FJ, Moreno D, Piccoli P, Hespanhol-Viana L, Silva MF, Bressan-Smith R, Cavagnaro JB, Bottini R (2010) Abscisic acid is involved in the response of grape (Vitis vinifera L.) cv. Malbec leaf tissues to ultraviolet-B radiation by enhancing ultraviolet-absorbing compounds, antioxidant enzymes and membrane sterols. Plant Cell Environ 33:1–10

  5. Bischof K, Kräbs G, Wiencke C, Hanelt D (2002) Solar ultraviolet radiation affects the activity of ribulose-1,5-bisphosphate carboxylase-oxygenase and the composition of photosynthetic and xanthophyll cycle pigments in the intertidal green alga Ulva lactuca L. Planta 215:502–509

  6. Cakmak I, Horst WJ (1991) Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiol Plant 83:463–468

  7. Cantarello C, Volpe V, Azzolin C, Bertea C (2005) Modulation of enzyme activities and expression of genes related to primary and secondary metabolism in response to UV-B stress in cucumber (Cucumis sativus L.). J Plant Interact 1:151–161

  8. Chang C-C, Yang M-H, Wen H-M, Chern J-C (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10:178–182

  9. Choudhary M, Jetley UK, Khan MA, Zutshi S, Fatma T (2007) Effect of heavy metal stress on proline, malondialdehyde, and superoxide dismutase activity in the cyanobacterium Spirulina platensis-S5. Ecotoxicol Environ Saf 66:204–209

  10. Cloix C, Jenkins GI (2008) Interaction of the Arabidopsis UV-B-specific signaling component UVR8 with chromatin. Mol Plant 1:118–128

  11. Cui D, Mo Y, Zeng L, Feng K, Feng X, Huang J, He M, Zhang X, Teng XJJ (2019) Response of Dendrobium officinale Kimura et Migo, a prized medicinal plant, to continuous UV-B irradiation at different C/N ratios. J Plant Growth Regul 39:358–369

  12. de Menezes HD, Massola NS Jr, Flint SD, Silva GJ Jr, Bachmann L, Rangel DE, Braga GU (2015) Growth under visible light increases conidia and mucilage production and tolerance to UV-B radiation in the plant pathogenic Fungus Colletotrichum acutatum. Photochem Photobiol 91:397–402

  13. Dias MC, Pinto DC, Freitas H, Santos C, Silva AMJP (2020) The antioxidant system in Olea europaea to enhanced UV-B radiation also depends on flavonoids and secoiridoids. Phytochemistry 170:112199

  14. Erkan M, Wang SY, Wang CY (2008) Effect of UV treatment on antioxidant capacity, antioxidant enzyme activity and decay in strawberry fruit. Postharvest Biol Technol 48:163–171

  15. Fales FW (1951) The assimilation and degradation of carbohydrates by yeast cells. J Biol Chem 193:113–124

  16. Farzadfar S, Zarinkamar F, Hojati M (2017) Magnesium and manganese affect photosynthesis, essential oil composition and phenolic compounds of Tanacetum parthenium. Plant Physiol Biochem 112:207–217

  17. Fortuna-Perez AP, de Moraes Castro M, de Azevedo Tozzi AMG (2012) Leaflet secretory structures of five taxa of the genus Zornia JF Gmel. (Leguminosae, Papilionoideae, Dalbergieae) and their systematic significance. Plant Syst Evol 298:1415–1424

  18. Ghanem ME, Han R-M, Classen B, Quetin-Leclerq J, Mahy G, Ruan C-J, Qin P, Perez-Alfocea F, Lutts S (2010) Mucilage and polysaccharides in the halophyte plant species Kosteletzkya virginica: localization and composition in relation to salt stress. J Plant Physiol 167:382–392

  19. Gill PK, Sharma AD, Singh P, Bhullar SS (2001) Effect of various abiotic stresses on the growth, soluble sugars and water relations of sorghum seedlings grown in light and darkness. Bulg J Plant Physiol 27:72–84

  20. Gregory M, Baas P (1989) A survey of mucilage cells in vegetative organs of the dicotyledons. Isr J Bot 38:125–174

  21. Guo J, Wang M-H (2009) Characterization of the phenylalanine ammonia-lyase gene (SlPAL5) from tomato (Solanum lycopersicum L.). Mol Biol Rep 36:1579–1585

  22. Gupta S, Gupta V, Singh V, Varadwaj PK (2018) Extrapolation of significant genes and transcriptional regulatory networks involved in Zea mays in response in UV-B stress. Genes Genom 40:973–990

  23. Hara M, Oki K, Hoshino K, Kuboi T (2004) Effects of sucrose on anthocyanin production in hypocotyl of two radish (Raphanus sativus) varieties. Plant Biotechnol 21:401–405

  24. Hilal M, Parrado MF, Rosa M, Gallardo M, Orce L, Massa EM, González JA, Prado FE (2004) Epidermal lignin deposition in quinoa cotyledons in response to UV-B radiation. Photochem Photobiol 79:205–210

  25. Hodálová I, Mereďa P Jr, Mártonfi P, Mártonfiová L, Danihelka J (2008) Morphological characters useful for the delimitation of taxa within Viola subsect. Viola (Violaceae): a morphometric study from the West Carpathians. Folia Geobot 43:83–117

  26. Hong NT, Ibrahim NH (2012) Extraction and characterization of mucilage from leaves of Pereskia bleo (rose cactus)[ekstraksi dan karakterisasi getah daun kaktus mawar (Pereskia bleo)]. J Teknol Ind Pangan 23:210–216

  27. Jiao J, Gai Q-Y, Yao L-P, Niu L-L, Zang Y-P, Fu Y-J (2018) Ultraviolet radiation for flavonoid augmentation in Isatis tinctoria L. hairy root cultures mediated by oxidative stress and biosynthetic gene expression. Ind Crops Prod 118:347–354

  28. Kakani V, Reddy K, Zhao D, Sailaja K (2003) Field crop responses to ultraviolet-B radiation: a review. Agric For Meteorol 120:191–218

  29. Karioti A, Furlan C, Vincieri FF, Bilia AR (2011) Analysis of the constituents and quality control of Viola odorata aqueous preparations by HPLC-DAD and HPLC-ESI-MS. Anal Bioanal Chem 399:1715–1723

  30. Khudyakova AY, Kreslavski VD, Shmarev AN, Lyubimov VY, Shirshikova GN, Pashkovskiy PP, Allakhverdiev SI (2019) Impact of UV-B radiation on the photosystem II activity, pro-/antioxidant balance and expression of light-activated genes in Arabidopsis thaliana hy4 mutants grown under light of different spectral composition. J Photochem Photobiol B 194:14–20

  31. Kumar S, Pandey AK (2013a) Chemistry and biological activities of flavonoids: an overview. Sci World J 2013:162750

  32. Kumar S, Pandey AKJV (2013b) Phenolic content, reducing power and membrane protective activities of Solanum xanthocarpum root extracts. Vegetos 26:301–307

  33. Liu L-X, Xu S-M, Woo K (2005) Solar UV-B radiation on growth, photosynthesis and the xanthophyll cycle in tropical acacias and eucalyptus. Environ Exp Bot 54:121–130

  34. Luthria DL, Mukhopadhyay S, Krizek DT (2006) Content of total phenolics and phenolic acids in tomato (Lycopersicon esculentum Mill.) fruits as influenced by cultivar and solar UV radiation. J Food Compos Anal 19:771–777

  35. Mishra V, Srivastava G, Prasad SM, Abraham G (2008) Growth, photosynthetic pigments and photosynthetic activity during seedling stage of cowpea (Vigna unguiculata) in response to UV-B and dimethoate. Pestic Biochem Physiol 92:30–37

  36. Mohammadi Shahrestani M, Saeidi Mehrvarz S, Marcussen T, Yousefi N (2014) Taxonomy and comparative anatomical studies of Viola sect. Sclerosium (Violaceae) in Iran. Acta Bot Gallica 161:343–353

  37. Murphy TM, Vu H (1996) Photoinactivation of superoxide synthases of the plasma membrane from rose (Rosa damascena Mill.) cells. Photochem Photobiol 64:106–109

  38. Nazari M, Zarinkamar F, Shafaghat Z (2018a) Manganese modulates the physiological and biochemical responses of Mentha aquatica L. to ultraviolet radiation. J Trace Elem Med Biol 45:1–10

  39. Nazari M, Zarinkamar F, Niknam V (2018b) Changes in primary and secondary metabolites of Mentha aquatica L. exposed to different concentrations of manganese. Environ Sci Pollut Res 25:7575–7588

  40. Parra R, Cadena E, Flores CJA (2019) Maximum UV index records (2010–2014) in Quito (Ecuador) and its trend inferred from remote sensing data (1979–2018). Atmosphere 10:787

  41. Pérez-de-Luque A, Lozano MD, Cubero JI, González-Melendi P, Risueno MC, Rubiales D (2006) Mucilage production during the incompatible interaction between Orobanche crenata and Vicia sativa. J Exp Bot 57:931–942

  42. Rodríguez-Calzada T, Qian M, Strid Å, Neugart S, Schreiner M, Torres-Pacheco I, Guevara-González RG (2019) Effect of UV-B radiation on morphology, phenolic compound production, gene expression, and subsequent drought stress responses in chili pepper (Capsicum annuum L.). Plant Physiol Biochem 134:94–102

  43. Ryan KG, Swinny EE, Markham KR, Winefield C (2002) Flavonoid gene expression and UV photoprotection in transgenic and mutant Petunia leaves. Phytochemistry 59:23–32

  44. Santos I, Fidalgo F, Almeida JM, Salema R (2004) Biochemical and ultrastructural changes in leaves of potato plants grown under supplementary UV-B radiation. Plant Sci 167:925–935

  45. Sarghein SH, Carapetian J, Khara J (2011) The effects of UV radiation on some structural and ultrastructural parameters in pepper (Capsicum longum A. DC.). Turk J Biol 35:69–77

  46. Sharma AK, Sharma UK, Pandey AK (2017) Protective effect of Bauhinia variegata leaf extracts against oxidative damage, cell proliferation and bacterial growth. India Sect B Biol Sci 87:45–51

  47. Smirnoff N, Conklin PL, Loewus FA (2001) Biosynthesis of ascorbic acid in plants: a renaissance. Annu Rev Plant Biol 52:437–467

  48. Tsurunaga Y, Takahashi T, Katsube T, Kudo A, Kuramitsu O, Ishiwata M, Matsumoto S (2013) Effects of UV-B irradiation on the levels of anthocyanin, rutin and radical scavenging activity of buckwheat sprouts. Food Chem 141:552–556

  49. Verdaguer D, Jansen MA, Llorens L, Morales LO, Neugart S (2017) UV-A radiation effects on higher plants: exploring the known unknown. Plant Sci 255:72–81

  50. Woolfe ML, Chaplin MF, Otchere G (1977) Studies on the mucilages extracted from okra fruits (Hibiscus esculentus L.) and baobab leaves (Adansonia digitata L.). J Sci Food Agric 28:519–529

  51. Yannarelli GG, Noriega GO, Batlle A, Tomaro ML (2006) Heme oxygenase up-regulation in ultraviolet-B irradiated soybean plants involves reactive oxygen species. Planta 224:1154–1162

  52. Yao X, Liu Q (2006) Changes in morphological, photosynthetic and physiological responses of Mono Maple seedlings to enhanced UV-B and to nitrogen addition. Plant Growth Regul 50:165

  53. Yao Y, Xuan Z, Li Y, He Y, Korpelainen H, Li C (2006) Effects of ultraviolet-B radiation on crop growth, development, yield and leaf pigment concentration of tartary buckwheat (Fagopyrum tataricum) under field conditions. Eur J Agron 25:215–222

  54. Zhang X-R, Chen Y-H, Guo Q-S, Wang W-M, Liu L, Fan J, Cao L-P, Li CJJ (2017) Short-term UV-B radiation effects on morphology, physiological traits and accumulation of bioactive compounds in Prunella vulgaris L. J Plant Interact 12:348–354




Special thanks to Tarbiat Modares University for funding this study.

Author Information

Shafaghat Zahra
Department of Plant Biology, Faculty of Biological Biology, Tarbiat Modares University, Tehran, Iran

Zarinkamar Fatemeh
Department of Plant Biology, Faculty of Biological Biology, Tarbiat Modares University, Tehran, Iran

Nazari Mehrdad
Department of Plant Biology, Faculty of Biological Biology, Tarbiat Modares University, Tehran, Iran