Whole proteome analysis of xero-halophyte Atriplex under salinity

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Research Articles | Published:

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Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00461-9
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Keywords: Atriplex , Comparative proteomics, Salt stress, Signaling, Xero-halophyte


Abstract


Salt stress is among the prime abiotic stresses that adversely affects crop yield worldwide. Halophytes are able to tolerate high salt concentrations, but a little information is available about their salt-adaptation mechanism. In the present study, we have investigated the molecular mechanism of salinity tolerance in Atriplex griffithii, an important xero-halophytic plant. Two-dimensional gel electrophoresis based comparative proteomics method was used to evaluate proteins’ expression in A. griffithii under control (0 mM NaCl) and saline (400 mM NaCl) conditions. Our results revealed that A. griffithii has strong potential to tolerate high concentration of salt, i.e., up to 400 mM. A total of 170 proteins were observed in both control and treatment gels, and 61 protein spots exhibited significant change of minimum twofold expression after imposing salt stress, with 11 novel salt-induced proteins. Maximum protein spots were having molecular weight and isoelectric point in a range of 14–110 kDa and 4–7, respectively. This study has revealed some important aspects of halophytic salt-stress responses at global proteome level. Our physiological data for proline content, lipid peroxidation and anti-oxidant enzymes activity also supported the salt-tolerant nature of this species. The differentially expressed proteins found in our study may be utilized to enhance abiotic stress tolerance in the sensitive agricultural crops through biotechnological techniques.



              Atriplex
            , Comparative proteomics, Salt stress, Signaling, Xero-halophyte


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Acknowledgements


The author gratefully acknowledges University Grants Commission, Government of India for UGC-BSR Start-up research grant (F.30-50/2014/BSR) to provide financial support for the research. The author is thankful to Ms. Chaya Chouhan for growing plants for the present study. The author also acknowledges the Department of Science and Technology, Government of India for the DST-SERB Young Scientist grant (SB/YS/LS-39/2014), UGC-CAS program, and DST-FIST program in Department of Botany, J.N.V. University, Jodhpur (India) for providing basic infrastructural facilities.


Author Information


Jha Shweta
Plant Functional Genomics Lab, Biotechnology Unit, Department of Botany (UGC-Centre of Advance Study), Jai Narain Vyas University, Jodhpur, India
jha.shweta80@gmail.com