Expression and in silico analysis of the pineapple SERK gene homologues during in vitro regeneration and induced Fusarium infection

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Pal, Soumili, Gangopadhyay, Gaurab


Research Articles | Published: 18 February, 2022

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00350-1
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Keywords: Pineapple, n AcSERK homologues, Docking analysis, n Fusariumn , qRT-PCR


Abstract


The Somatic Embryogenesis Receptor Kinase (SERK) gene serves a dual purpose in plants. In addition to the induction of somatic embryogenesis, it also triggers a line of host defence against plant pathogens. The AcSERK gene family contains three homologues, viz. AcSERK1, AcSERK2, and AcSERK3 in pineapple [Ananus comosus (L.) Merr.]. We are reporting the in-silico analysis of these homologues to find out their predicted function in pathogen defence. Of the three homologues, AcSERK3 showed a unique extra leucine-rich repeat domain at the N-terminal region. It provides a high inferred function against abiotic stress concerning AcSERK1 and AcSERK2. We have also studied the expression pattern of the gene homologues in pineapple plants in response to the induced infection with Fusarium through qRT-PCR analysis. Fusarium moniliforme var. subglutinans is the causal organism of deadly "Fusariosis" disease in pineapple. The expression of AcSERK3 among all three homologues was most pronounced in the pathogen-infected plants starting from meristem to the leaves of the outer whorl. AcSERK1 was also expressed throughout the outermost leaf to meristem, and it may act as a go-between of pathogen-defense signal pathway. However, the expression of AcSERK2 was limited to the meristem region in both the control and infected plants. Since the expression of AcSERK3 was statistically significant in the infected plant, we conclude that it is the most potent homologue of the AcSERK gene.

Pineapple, n                     AcSERK homologues, Docking analysis, n              Fusariumn            , qRT-PCR


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Acknowledgements


We thank the Director of Bose Institute for providing financial and infrastructural support. The first author (SP) acknowledges the financial assistance in form of research fellowship provided by DST (Department of Science and Technology, India)-INSPIRE division (Innovation in Science Pursuit for Inspired Research). We also acknowledge the technical assistance of Mr. Jadab Ghosh and Mrs. Kaberi Ghosh. GG is also thankful to the Department of Science & Technology and Biotechnology, Government of West Bengal (0496/RND/BIOSCI/Nil/Mar-2021/1/1) for funding a major portion of this work.


Author Information


Pal, Soumili
Division of Plant Biology, Bose Institute, Kolkata, India

Gangopadhyay, Gaurab
Division of Plant Biology, Bose Institute, Kolkata, India
gaurab@jcbose.ac.in