Toxicological assessments of aqueous extract of UV-C irradiated Bacopa floribunda leaves in healthy male albino rat

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Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-023-00616-2
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Keywords: n Bacopa floribundan , Heamatopoeitic, Irradiation, Oxidative stress, Subacute


Abstract


Consuming medicinal plants for the presumed health benefits have been a common practice since early civilization and study is ongoing in improving the activity of the active constituents as well as their toxic effect. This study investigated the toxicological effect of oral administration of non-irradiated and irradiated Bacopa floribunda in healthy male albino rats. Standard acute testing was adopted while in subacute study, 35 healthy male rats were randomly divided into two treatments categories of three dosing groups which were 100, 400 and 800 mg/kg of extracts as well as the control. Hematopoietic functions, biochemical parameters, oxidative stress marker and organ histology were studied. Acute study revealed that the lethal dose of the extracts was above 5000 mg/kg. Daily dosing for 14 days caused significant reduction in mean organ body weight ratio of rats administered with irradiated leaves extract. Hematological parameters were not affected by B. floribunda leaves extracts. Except for urea that was significantly (p < 0.05) elevated in treated groups, other kidney function and liver markers remain unchanged compared to the control. Both extracts concomitantly reduced cholesterol and LDL concentrations. SOD activity and GSH were not adversely affected by both extract while CAT activity was altered in selected groups that received irradiated leave extract. Doses of irradiated leave extract slightly alter liver and kidney architectures while non-irradiated leave extract showed no sign of toxicity. In conclusion, this study has demonstrated that non-irradiated and irradiated Bacopa floribunda leaves are practically safe.


n              Bacopa floribundan            , Heamatopoeitic, Irradiation, Oxidative stress, Subacute


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References


Abbasi BH, Khan T, Khurshid R, Nadeem M, Drouet S, Hano C (2021) UV-C mediated accumulation of pharmacologically significant phytochemicals under light regimes in in vitro culture of Fagonia indica (L.). Sci Rep 11:679. https://doi.org/10.1038/s41598-020-79896-6


Adetuyi FO, Karigidi KO, Akintimehinm ES, Fajembola TF (2019) Effect of postharvest UV-C irradiation as physical elicitor on anti-nutritional factor, B-vitamins and mineral profile of Clerodendrum volubile leaves. Croatian J Food Technol Biotechnol Nutr 14(3–4):113–120


Adetuyi FO, Karigidi KO, Akintimehin ES (2020) Effect of postharvest UV-C treatments on the bioactive components, antioxidant and inhibitory properties of Clerodendrum volubile leaves. J Saudi Soc Agric Sci 19:7–13. https://doi.org/10.1016/j.jssas.2018.03.005


Adetuyi FO, Akintimehin ES, Karigidi KO (2022) Comparative analysis of freshly harvested and stored Bacopa floribunda leaves: HPLC phenolic fingerprinting, antioxidant and cholinergic enzyme inhibition properties. Adv Tradit Med. https://doi.org/10.1007/s13596-021-00626-y


Ajuwon OR, Oguntibeju OO, Marnewick JL (2014) Amelioration of lipopolysaccharide-induced liver injury by aqueous rooibos (Aspalathus linearis) extract via inhibition of pro-inflammatory cytokines and oxidative stress. BMC Complement Altern Med 14:392. https://doi.org/10.1186/1472-6882-14-392


Akintimehin ES, Karigidi KO, Omogunwa TS et al (2021) Safety assessment of oral administration of ethanol extract of Justicia carnea leaf in healthy wistar rats: hematology, antioxidative and histology studies. Clin Phytosci 7:2. https://doi.org/10.1186/s40816-020-00234-4


Bode AM, Dong Z (2014) Toxic phytochemicals and their potential risks for human. Cancer Prev Res. https://doi.org/10.1158/1940-6207.CAPR-14-0160


Brígido HPC, Everton LPV, Antônio RQG, Mirian LCB, de Andre OF, Andrey MRM, Liliane AC et al (2021) Evaluation of acute and subacute toxicity of ethanolic extract and fraction of alkaloids from bark of Aspidosperma nitidum in mice. Sci Rep 11:18283. https://doi.org/10.1038/s41598-021-97637-1


Chanda S, Parekh J, Vaghasiya Y, Dave R, Baravalia Y, Nair R (2015) Medicinal plants—from traditional use to toxicity assessment: a review. Int J Pharm Sci Res 6(7):2652–2670


Cohen G, Dembiec D, Marcus J (1970) Measurement of catalase activity in tissue extracts. Anal Biochem 34(1):30–38


Dhaliya SA, Surya AS, Dawn VT, Carla B, Arun K, Sunil C (2013) A review of hyperlipidemia and medicinal plants. Int J Pharm Sci Biomed Sci 2(4):219–237


Donkor K, Okine LNK, Abotsi WKM, Woode E (2014) Acute and sub-chronic toxicity studies of aqueous extract of root bark of Cassia sieberiana DC in rodents. J Appl Pharm Sci 4(4):84–89. https://doi.org/10.7324/JAPS.2014.40415


Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77


Ferrier P (2010) Irradiation as a quarantine treatment. Food Policy 35:548–555. https://doi.org/10.1016/j.foodpol.2010.06.001


Gatta A, Verardo A, Bolognesi M (2012) Hypoalbuminemia. Intern Emerg Med 7(Suppl 3):S193–S199. https://doi.org/10.1007/s11739-012-0802-0


Kazemipoor M, Cordell GA, Sarker MR, Mohamed Radzi CWJW, Hajifaraji M, En Kiat P (2015) Alternative treatments for weight loss: safety/risks and effectiveness of anti-obesity medicinal plants. Int J Food Prop 18(9):1942–1963. https://doi.org/10.1080/10942912.2014.933350


Kumar A, Kumar B, Kumar R, Ajay K, Singh M, Tiwari V, Trigunayat A, Paul P, Singh P (2022) Acute and subacute toxicity study of ethanolic extract of Calotropis procera (Aiton) dry and flower in Swiss albino mice. Phytomed Plus 2:100224. https://doi.org/10.1016/j.phyplu.2022.100224


Li XR et al (2010) Acute and subacute toxicity of ethanol extracts from Salvia przewalskii Maxim in rodents. J Ethnopharmacol 131(1):110–115. https://doi.org/10.1016/j.jep.2010.06.012


Lorke D (1983) A new approach to practical acute toxicity testing. Arch Toxicol 53:275–289


Lushchak VI (2012) Glutathione homeostasis and functions: potential targets for medical interventions. J Amino Acids 2012:736837


Misra HP, Fridovich I (1972) The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247(10):3170–3175


Nandi A, Liang-Jun Y, Chandan KJ, Das N (2019) Role of catalase in oxidative stress- and age-associated degenerative diseases. Oxid Med Cell Longevity. https://doi.org/10.1155/2019/9613090


National Research Council (1997) Occupational health and safety in the care and use of research animals. The National Academies Press, Washington, DC


Nwonuma CO, Osemwegie OO, Alejolowo OO, Irokanulo EO, Olaniran AF, Fadugba DO, Opaleke DO, Ojo OA (2021) Antioxidant and the ameliorating effect of Allium cepa (Onion) fortified feed against potassium bromate induced oxidative damage in Wistar rats. Toxicol Rep 8:759–766


Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95(2):351–358


Olaniyan JM, Muhammad HL, Makun HA, Busari MB, Abdullah AS (2016) Acute and sub-acute toxicity studies of aqueous and methanol extracts of Nelsonia campestris in rats. J Acute Dis 5(1):62–70


Omodanisi EI, Aboua YG, Oguntibeju OO, Lamuela-Raventós RM (2017) Assessment of the anti-hyperglycaemic, anti-inflammatory and antioxidant activities of the methanol extract of moringa oleifera in diabetes-induced nephrotoxic male wistar rats. Molecules 22:439


Roberts S, James RC, Franklin MR (2003) Hepatotoxicity: toxic effects on the liver. In: Williams PL, James RC, Roberts SM (eds) Principles of toxicology: environmental and industrial applications, 2nd edn. John Wiley & Sons, Inc., New York, pp 111–128


Sharif HB, Mukhtar MD, Mustapha Y, Baba G, Lawal AO (2015) Acute and subchronic toxicity profile of Euphorbia pulcherrima methanol extract on wistar albino rats. Adv Pharm. https://doi.org/10.1155/2015/539646


Sireeratawong S, Jaijoy K, Khonsung P, Lertprasertsuk N, Ingkaninan K (2016) Acute and chronic toxicities of Bacopa monnieri extract in Sprague Dawley rats. BMC Complement Altern Med 16:249. https://doi.org/10.1186/s12906-016-1236-4


Soeters PB, Wolfe RR, Shenkin A (2019) Hypoalbuminemia: pathogenesis and clinical significance. J Parenter Enter Nutr 43(2):181–193


Sotler R, Poljšak B, Dahmane R, Jukić T, Jukić DP, Rotim C, Trebše P, Starc A (2019) Prooxidant activities of antioxidants and their impact on health. Acta Clin Croat 58:726–736. https://doi.org/10.20471/acc.2019.58.04.20


Tamilselvan N, Thirumalai T, Shyamala P, David E (2014) A review on some poisonous plants and their medicinal values. J Acute Dis 3(2):85–89. https://doi.org/10.1016/S2221-6189(14)60022-6


Tietz NW (1995) Clinical guide to laboratory tests, 3rd edn. WB. Saunders, Philadelphia, pp 268–273


Tsai CF, Hsu YW, Chen WK, Chang WH, Yen CC, Ho YC, Lu FJ (2009) Hepato-protective effect of electrolyzed reduced water against carbon tetrachloride-induced liver damage in mice. Food Chem Toxicol 47(8):2031–2036


Turtoi M (2013) Ultraviolet light treatment of fresh fruits and vegetables surface: a review. J Agroaliment Process Technol 19(3):325–337


Urban L et al (2018) UV-C light and pulsed light as alternatives to chemical and biological elicitors for stimulating plant natural defenses against fungal diseases. Sci Hortic 235:452–459


Zhou Y, Shen YH, Zhang C, Zhang WD (2007) Chemical constituents of Bacopa monnieri. Chem Nat Compd 43(3):355–357

 


Acknowledgements


The authors specially thank the Tertiary Education Trust Fund (TETFUND) of the Federal Government of Nigeria for funding this research work. YEAR(S) 2020 (MERGED) TETFUND INTERVENTION IN RESEARCH PROJECT (RP). OAUSTECH/TETFund/VOL.1/001.


Author Information


Adetuyi Foluso O.
Department of Chemical Sciences (Biochemistry Unit), School of Science, Olusegun Agagu University of Science and Technology, Okitipupa, Nigeria
foluadetuyi@yahoo.co.uk
Akintimehin Emmanuel S.
Department of Chemical Sciences (Biochemistry Unit), School of Science, Olusegun Agagu University of Science and Technology, Okitipupa, Nigeria


Karigidi Kayode O.
Department of Chemical Sciences (Biochemistry Unit), School of Science, Olusegun Agagu University of Science and Technology, Okitipupa, Nigeria