Evaluation of COX-2 production, anticancer efficacy against MCF-7 cell lines, and antioxidant activity of Garcinia conicarpa, a novel endemic species of the Western Ghats

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Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-023-00765-4
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Keywords: n Garcinia conicarpan , HR LC–MS, Antioxidant activity, Anti-inflammatory, Anticancer


Abstract


Garcinia species are members of the Cluciaceae family and are rich in secondary metabolites. In the Western Ghats, Garcinia conicarpa is a recently discovered endemic species. In this study, the methanolic leaf extract of Garcinia conicarpa (GC) is examined for its anti-inflammatory, anti-cancer, and antioxidant properties. Significant phytocompounds were discovered using HR LC–MS. To investigate the antioxidant capacity of extracts, four different radical scavenging assays, such as the hydroxyl radical scavenging assay, the nitric oxide radical scavenging assay, the ABTS radical scavenging assay, and the DPPH radical scavenging assay, were used. Through assays such as inhibition of protein denaturation, proteinase inhibitory activity, cycloxygenase (COX) activity, lipoxygenase (LOX) activity, and inducible nitric oxide synthase activity assays, G. conicarpas anti-inflammatory properties were assessed. While anti-proliferative activity was studied using breast cancer MCF-7 cell lines. The activity of important compounds isolated through HR LC–MS was analysed. This is the first time the antioxidant activity of the methanolic leaf extract of G. conicarpa has been investigated. Leaf extract shows high to medium antioxidant activity in all the assays. G. conicarpa also has anti-inflammatory activity in a dose-dependent manner in all the biochemical assays, and the extract reduced the cyclooxygenase enzyme (COX-2) level from 0.84 to 0.75 U/mL. In the anti-cancerous study, the cell viability percentage of MCF-7 cell lines ranged from 95.7% to 71.8%. So, it is evident from this study that G. conicarpa methanolic leaf extract is a unique natural anti-inflammatory and antioxidant treasure with the capability to combat breast cancer.


n                     Garcinia conicarpan                  , HR LC–MS, Antioxidant activity, Anti-inflammatory, Anticancer


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Acknowledgements


The first author gratefully acknowledges University grant commission (UGC) Government of India or the award of the fellowship.


Author Information


Divyalakshmi M. V.
Cell and Molecular Biology Division, Department of Botany, University of Calicut, Kerala, India
mvdivyalakshmi@gmail.com
Thoppil J. E.
Cell and Molecular Biology Division, Department of Botany, University of Calicut, Kerala, India
jethoppil@gmail.com