Genetic diversity and relationship between Egyptian Vigna (Vigna spp. (L.) Walp.) taxa populations via Phenotypic and Molecular profiling

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Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-024-00827-1
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Keywords: Amino acid, DNA barcoding, Partial rbcL, UPLC


Abstract


Genetic diversity is crucial for the conservation and improvement of plant populations. In this study, we used molecular and phenotypic profiling to determine the genetic diversity and relationships among Egyptian Vigna populations, including V. radiata, V. unguiculata, and V. luteola. The samples were profiled using amino acids and rbcL sequences, and the data were analyzed to determine the similarities among the taxa. The results showed a high degree of similarity between V. radiata and V. unguiculata, followed by V. luteola and V. unguiculata, and V. luteola and V. radiata. These taxa are closely related to V. unguiculata subsp. cylindrical and V. oblongifolia. Our findings suggest that molecular- and phenotypic-based profiling can help in prioritizing different disciplines based on assigning them to the proper taxonomic position through extra collections and management. These findings have practical implications for the management of Egyptian Vigna populations, as they can inform conservation and crop improvement efforts. Further research in this area could help preserve the wealth of germplasm for biodiversity and contribute to the sustainable use of plant resources.


Amino acid, DNA barcoding, Partial rbcL, UPLC


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Acknowledgements


We thank Prof. Reda M. Rizk, Arab Organization for Agricultural Development, League of Arab States, for his efforts to verification. We acknowledge and appreciate the comments of anonymous reviewers and technical Journals’ editors.


Author Information


Mahdy Ehab M. B.
National Gene Bank, Agricultural Research Center, Giza, Egypt
ehab.mahdy@arc.sci.eg
Ibrahim Shafik D.
Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza, Egypt
Shafiqdarwish2014@gmail.com

EL-Shaer Hosam F.
Faculty of Agriculture, Al-Azhar University, Cairo, Egypt

hosamelshaer805@yahoo.com
Mansour Mohamed S.
National Gene Bank, Agricultural Research Center, Giza, Egypt

mohamed.mansour@arc.sci.eg