Vegetos
(An International Journal of Plant Research & Biotechnology)
Influence of culture media and growth hormones on in vitro propagation of Adinandra griffithii Dyer., a critically endangered and endemic plant from northeast India
*Article not assigned to an issue yet
Research Articles | Published: 23 April, 2024
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
First Page: 0
Last Page: 0
Views: 242
Keywords:
n Adinandra griffithiin , Endemic, In vitro, Shoot proliferation, Ex situ conservation
Abstract
An effective and reliable in vitro plant regeneration method has been established for Adinandra griffithii Dyer., a critically endangered species which is endemic to Northeast India. This study presents the first successful micropropagation protocol for the Pentaphylaceae family in India. The axenic cultures were established through in vitro seed germination. Seeds inoculated in Woody Plant Medium showed better germination percentage (94%) compared to the seeds inoculated in Murashige and Skoog Medium (66.8%) with an average number of 9.4 ± 0.21a seeds in WPM and 6.68 ± 0.18b seeds in MS Medium. The highest shoot proliferation of 8.0 ± 0.26a shoots per explant with 2.59 ± 0.06a cm mean shoot length was recorded in WPM supplemented with 6-BAP at 6 mg L-1. The best results for in vitro rooting were obtained from WPM augmented with IBA at 1 mg L-1 where 14.5 ± 0.72a roots per shoot were formed with an average root length of 1.27 ± 0.07a cm. The in vitro raised plants were successfully acclimatized in greenhouse and transferred to field with 70% survival rate. The healthy acclimatized plants were reintroduced in different Northeastern states like Meghalaya and Nagaland. The protocol developed is beneficial for ex situ conservation of germplasm and mass production of A. griffithii plants.
(*Only SPR Members can get full access. Click Here to Apply and get access)
References
Adhikari D, Mir AH, Upadhaya K, Iralu V, Roy DK (2018) Abundance and habitat-suitability relationship deteriorate in fragmented forest landscapes: a case of Adinandra griffithii Dyer, a threatened endemic tree from Meghalaya in northeast India. Ecol Process 7:3. https://doi.org/10.1186/s13717-018-0114-z
Aniat-ul-Haq RV, Agnihotri RK (2010) Effect of osmotic stress (PEG) on germination and seedling survival of lentil (Lens culinaris Medik). Res J Agric Sci 1(3):201–204
Banjara K, Swamy SL, Singh AK (2017) Vegetative propagation of Terminalia arjuna (Roxb.) WT. & ARN. by stem cuttings under mist. Int J Agric Sci 9(50):4847–4850
Champion HG, Seth SK (2005) A revised survey of the forest types of India. Government of India. Natraj, New Delhi
Demétrio CA, Jacob JFO, Ambrosano GB, Rodrigues PHV (2022) In vitro germination of Abricó-de-Macaco (Couroupita guianensis aubl.) Zygotic embryos in different culture media and light spectra. Cienc Rural 52:1. https://doi.org/10.1590/0103-8478cr20190906
Gehlot A, Gupta RK, Tripathi A, Arya I, Arya S (2014) Vegetative propagation of Azadirachta indica: effect of auxin and rooting media on adventitious root induction in mini-cuttings. Adv Sci 1(1):106–115
George EF (1993) Plant propagation by tissue culture. The technology, 2nd edn. England, Exegetics Ltd
Gonbad RA, Sinniah UR, Aziz MA, Mohamad R (2014) Influence of cytokinins in combination with GA3 on shoot multiplication and elongation of tea clone Iran 100 (Camellia sinensis (L.) O. Kuntze). Sci World J. https://doi.org/10.1155/2014/943054
Goyal KA, Pradhan S, Basistha CB, Sen A (2015) Micropropagation and assessment of genetic fidelity of Dendrocalamus strictus (Roxb.) Using RAPD and ISSR markers. 3 Biotech 5:473–482. https://doi.org/10.1007/s13205-014-0244-7
Ibrahim ME, Mohamed MA, Khalid KA (2015) Effect of plant growth regulators on rooting of lemon verbena cuttings. Mater Environ Sci 6(1):28–33
IBP (2019) Indian Biodiversity Portal. Adinandra griffithii Dyer. https://indiabiodiversity.org/species/show/226499. Accessed 15 Mar 2023
IUCN (2020) The IUCN Red List of Threatened Species Version 2020-3. https://www.iucnredlist.org. Accessed 15 Mar 2023
Kadota M, Niimi Y (2003) Effects of cytokinin types and their concentrations on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots. Plant Cell Tissue Organ Cult 72:261–265. https://doi.org/10.1023/A:1022378511659
Khan MI, Ahmad N, Anis M (2011) The role of cytokinins on in vitro shoot production in Salix Tetrasperma Roxb.: a tree of ecological importance. Trees 25:577–584. https://doi.org/10.1007/s00468-010-0534-6
Kumar S, Chandra A, Gupta MG (2008) Plantlet regeneration via multiple shoot induction in Indian accessions of lucerne (Medicago sativa L). J Plant Biochem Biot 17:181–184. https://doi.org/10.1007/BF03263282
Lasushe K, Mir AH, Singh PP, Chaudhary KL, Choudhury H, Deori C, Roy DK, Singh B, Upadhaya K (2022) A comprehensive checklist of threatened plants of Meghalaya, Northeast India. J Asia-Pac Biodivers 15:435–441. https://doi.org/10.1016/j.japb.2022.03.008
Mathur S (2013) Conservation of Biodiversity through tissue culture. J Microbiol Biotechnol 2(3)
McCown BH, Lloyd GB (1981) Woody Plant Medium (WPM)—A Mineral nutrient formulation for microculture of Woody Plant species. HortScience 16:453–453
Mir AH (2017) Plant diversity assessment in community forests for priority conservation in Khasi Hills, Meghalaya. North-Eastern Hill University
Mir AH, Upadhaya K, Jeri L (2020) Adinandra griffithii. The IUCN Red List of Threatened Species. https://doi.org/10.2305/IUCN.UK.2020-3.RLTS.T32924A175522046.en
Mondal TK, Bhattacharya A, Sood A, Ahuja PS (1998) Micropropagation of tea (Camellia sinensis (L.) O. Kuntze) using thidiazuron. Plant Growth Regul 26(1):57–61. https://doi.org/10.1023/A:1006019206264
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nowakowska K, Pacholczak A, Tepper W (2019) The effect of selected growth regulators and culture media on regeneration of Daphne mezereum L. ‘Alba’. Rend Fis Acc Lincei 30:197–205. https://doi.org/10.1007/s12210-019-00777-w
Polivanova OB, Bedarev VA (2022) Hyperhydricity in plant tissue culture. Plants 11:3313. https://doi.org/10.3390/plants11233313
Park SY, Kim YW, Moon HK, Murthy HN, Choi YH, Cho HM (2008) Micropropagation of Salix pseudolasiogne from nodal explants. Plant Cell Tissue Organ Cult 93:341–346. https://doi.org/10.1007/s11240-008-9362-4
Phuyal N, Jha PK, Raturi PP, Gurung S, Rajbhandary S (2018) Effect of growth hormone and growth media on the rooting and shooting of Zanthoxylum armatum stem cuttings. Banko Janakari 28(2):3–12
Rahman A, Bannigan A, Sulaman W, Pechter P, Blancaflor EB, Baskin TI (2007) Auxin, actin and growth of the Arabidopsis thaliana primary root. Plant J 50(3):514–528. https://doi.org/10.1111/j.1365-313x.2007.03068.x
Rivers MC, Shaw K, Beech E, Jones M (2015) Conserving the World’s most threatened trees: a global survey of ex situ collections. Botanic Gardens Conservation International, Richmond, UK
Sadeghi F, Yadollahi A, Kermani JM, Eftekhari M (2015) Optimizing culture media for in vitro proliferation and rooting of tetra (Prunus empyrean 3) rootstock. J Genet Eng Biotechnol 13:19–23. https://doi.org/10.1016/j.jgeb.2014.12.006
Shahzad AA, Sharma S, Parveen S, Saeed T, Shaheen A, Akhtar R, Yadav V, Upadhyay A, Ahmad Z (2017) In: Abdin M, Kiran U, Kamaluddin, Ali A (eds) Plant Biotechnology: principles and applications. Springer, Singapore, pp 1–36. https://doi.org/10.1007/978-981-10-2961-5_1
Siwach P, Gill RA (2011) Enhanced shoot multiplication in Ficus religiosa L. in the presence of adenine sulphate, glutamine, and phloroglucinol. Physiol Mol Biol Plants 17:271–280. https://doi.org/10.1007/s12298-011-0074-6
Trivedi DR, Joshi AG (2014) Studies on seed germination of Stereospermum suaveolens with respect to different parameters. Environ Exp Biol 12:33–37
Upadhaya K, Mir AH, Iralu V (2017) Reproductive phenology and germination behavior of some important tree species of Northeast India. Proc Nat Acad Sci India Sect B: Biol Sci 88:1033–1041. https://doi.org/10.1007/s40011-017-0841-4
Wojtania A, Gabryszewska E, Podwyszynska M (2011) The effect of growth regulators and sucrose concentration on in vitro propagation of Camellia japonica L. Propag Ornam Plants 11:177–183
Yamaguchi S (2008) Gibberellin metabolism and its regulation. Ann Rev Plant Biol 59:25–251. https://doi.org/10.1146/annurev.arplant.59.032607.092804
Yeung ECT, Stasolla C, Sumner MJ, Huang BQ (2015) Plant microtechniques and protocols. Nova York. https://doi.org/10.1007/978-3-319-19944-3
Andrade L, Echeverrigaray S, Fracaro F, Pauletti GF, Rota L (1999) The effect of growth regulators on shoot propagation and rooting of common lavender (Lavandula vera DC). Plant Cell, Tissue Organ Cult 56(2):79–83. https://doi.org/10.1023/A:1006299410052
Gribaudo I, Fronda A (1991) Effects of thidiazuron on grapevine axillary buds cultivated in vitro. HortScience 26(8):1083
Acknowledgements
The first author expresses gratitude to the Director of Botanical Survey of India, Kolkata, for granting fellowship as part of the Flora of India project as well as for the encouragement and facilities. The authors also extend their appreciation to the Head of Office, Botanical Survey of India, Eastern Regional Centre, Shillong for delivering necessary resources and support.
Author Information