In vitro studies in red dacca (Musa acuminata): an ornamental horticultural crop

*Article not assigned to an issue yet

, , , , , ,


Short Communications | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-024-00912-5
First Page: 0
Last Page: 0
Views: 457


Keywords: n Musa acuminaten , Wild species, Micropropagation, Murashige and skoog medium, 6-Benzyl amino purine (BAP), Indole-3-butyric acid, Hardening


Abstract


Bananas (Musa) are non-woody, giant herbaceous perennial plants well-acquainted for their nutritious fruits and utile leaves. Cultivation practices used for crop improvement provide not only food security but also create employment opportunities to the growers. Red banana (Musa acuminata) not only possesses nutritive value but also decorates the ecology bearing red-colored inflorescence; which suits it well as an ornamental plant. To broaden our knowledge of red banana cultivation, we, therefore, targeted our research on this wild cultivar to achieve its high rate of production by standardizing in-vitro conditions of micropropagation. Axillary buds were used as explants for in vitro plant regeneration studies in Musa acuminata. MS media supplemented with growth regulators BAP (4 mg l−1), and IBA (1 mg l−1) suited well for 80% multiplication response and 63% root regeneration in the red banana cultures. Obtained plantlets were further acclimatized for hardening in the potting mixture of soil:FYM:sand (1:1:1) with a 92% survival rate. The current work executed for clonal propagation of this wild cultivar provides a forum for further research in molecular breeding programs.


n                     Musa acuminaten                  , Wild species, Micropropagation, Murashige and skoog medium, 6-Benzyl amino purine (BAP), Indole-3-butyric acid, Hardening


*Get Access

(*Only SPR Members can get full access. Click Here to Apply and get access)

Advertisement

References


Agastian P, Williams L, Ignacimuthu S (2006) In vitro propagation of Justicia gendarussa Burm. F—a medicinal plant. Indian J Biotechnol 5:246–248


Ali A, Sajid A, Naveed NH, Majid A, Saleem A, Khan UA, Fafery FI, Naz S (2011) Initiation, proliferation and development of micropropagation system for mass-scale production of banana through meristem culture. Afr J Biotechnol 10:15731–15738


Aman T, Prabhuling G, Hipparagi K, Prakash DP, Babu AG (2018) In vitro multiplication of banana cv. rajapuri bale (Musa spp., AAB group). Int J Curr Microbiol Appl Sci 7(7):3141–3151


Anbazhagan M, Balachandran B, Arumugam K (2014) In vitro propagation of Musa sp (Banana). Int J Curr Microbiol Appl Sci 3(7):399–404


Arias O (1992) Commercial micropropagation of banana. Biotechnology applications for banana and plantain improvement, INIBAP, SanJose, Costa Rica


Elhory SMA, Aziz MA, Rahid AA, Yunus AG (2009) Prolific plant regeneration through organogenesis from scalps of (Musa spp.) cv Tanduk. Afr J Biotechnol 8:6208–6213


Gilmar RZ, Gilberto BK, Jane EK, Elaine C (2000) Hormonal and histological studies related to in vitro banana bud formation. Plant Cell Tissue Organ Cult 63:187–192


Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research, 2nd edn. Wiley, New York, p 680


Hrahsel L, Basu A, Sahoo L, Robert T (2014) In vitro propagation and assessment of the genetic fidelity of Musa acuminata (AAA) cv. Vaibalhla derived from immature male flowers. Appl Biochem Biotechnol 172:1530–1539


Justine AK, Kaur N, Savita PPK (2022) Biotechnological interventions in banana: current knowledge and future prospects. Heliyon 8(11):e11636


Kumar KPS, Bhowmik D, Duraivel S, Manivannan U (2012) Traditional and medicinal uses of banana. J Pharmacogn Phytochem 1:51–63


Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497


Naika HR, Krishna V (2008) Plant regeneration from callus culture of Clematis gouriana Roxb.—a rare medicinal plant. Turk J Biol 32:99–103


Nandariyah et al (2021) Development of banana in vitro from male bud culture supplemented with some concentration of sucrose and benzyladenine. IOP Conf Ser: Earth Environ Sci 724:012007


Parkhe S, Dahale M, Ningot E, Gawande S (2018) Study of different potting mixture on hardening of banana tissue culture plantlets its field performance. Int J Curr Microbiol App Sci 6:1941–1947


Rahman MZ, Rahman MH, Mullah MU, Sultan RS, Bari MA, Hossain M (2005) In vitro shoot multiplication and rooting of a dessert banana (Musa sp cv. Anupom). Pak J Biol Sci 8:1298–1302


Rahman S, Biswas N, Hassan M, Ahmed G, Islam R, Moniruzzaman HE (2013) Micro propagation of red banana (Musa sp.) cv. Agnishwar. Int Res J Biotech 4(4):83–88


Rajoriya P, Singh V, Jaiswal N, Lall R (2018) Optimizing the effect of plant growth regulators on in vitro micropropagation of Indian red banana (Musa acuminata). J Pharmacogn Phytochem 7:628–634


Sawardekar S, Keskar K, Gokhale N, Sawant S (2018) Standardization of in vitro regeneration techniques in red banana and fidelity testing of tissue culture-raised plantlets of Red Banana. J Eco Env 37:101–109


Singh CR, Kathiresan K, Boopathy NS, Anandhan S, Govindan T (2013) Evaluation of microbial potential of different coloured banana peels. Int J Preclin Pharm Res 4:62–64


Subramaniam S, Rathinam X, Poobathy R, Sinniah U (2008) In vitro production of multiple bud clumps (Mbcs) from cavendish banana cultivar, Brasilian (AAA). Americ-Eura J Sust Agri 2(3):300–307


Uzaribara E, Nachegowda V, Ansar H, Sathyanarayana BN, Amreen T (2015) In vitro propagation of red banana (Musa acuminata). The Bioscan 10(1):125–129


Vuylsteke D (1989) Shoot-tip culture for the propagation conservation and exchange of Musa germplasm. IBPGR, Rome


Zaffari GR, Kerbauy GB, Kraus JE, Romano EC (2000) Hormonal and histological studies related to in vitro banana bud formation. Plant Cell Tissue Organ Cult 63(3):187–192

 


Acknowledgements


The authors would like to acknowledge the Shri. Aditya Biotech Laboratory Hosur, Tamil Nādu for providing the red banana explants for experimentation studies.


Author Information


Kamble Ravindra Siddheshwar
Lokmangal College of Agricultural Biotechnology Wadala, Solapur, India

Pawar Rohini
Lokmangal College of Agricultural Biotechnology Wadala, Solapur, India


Dishri Madhusmita
VAIAL - Vellore Institute of Technology, Vellore, India


Dobhal Sneha
VCSG Uttarakhand University of Horticulture and Forestry, Ranichauri, India


Kanungo Sourav
Lokmangal College of Agricultural Biotechnology Wadala, Solapur, India

Sharma Amit
RKMV, Shimla, India

Thakur Nisha
CSKHPKV, Palampur, India
nishathakur81086@gmail.com