A High Throughput, Improved Rapid and Reliable Genomic DNA Extraction Protocol From Chickpea (Cicer arietinum L.)

Kumar Tapan, Bharadwaj C.*, Satyavathi C. Tara, Jain P. K.

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.5958/j.2229-4473.26.2.073
First Page: 185
Last Page: 190
Views: 1220


Keywords: Genogrinder, DNA extraction, Chickpea, PCR assay.


Abstract

A high throughput, relatively rapid, reliable and inexpensive protocol for the genomic DNA isolation from chickpea is described. The procedure involved a modified cetyltrimethyl-ammonium bromide (CTAB) of and β-mercaptoethanol buffer protocol with the use of indigenous genogrinder for DNA extraction from 100 mg of mature and young leaves of chickpea. The present protocol is also well suited for extracting high-molecular weight DNA from leguminous plant materials rich in polyphenols, tannins and polysaccharides. The DNA obtained by the present protocol is of very good quality, with no coloured pigment and contaminants, without using expensive liquid nitrogen and toxic phenols.


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References


Acknowledgements


Author Information

Kumar Tapan Bharadwaj C.* Satyavathi C. Tara Jain P. K.

1National Research Centre for Biotechnology, Pusa Campus, New Delhi-110012

*Corresponding author Email: drchbharadwaj.iari@gmail.com

Online published on 9 January, 2014.