Alteration in physio-biochemical attributes and chlorophyll fluorescence of mungbean caused by arsenic stress

*Article not assigned to an issue yet

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-022-00421-3
First Page: 0
Last Page: 0
Views: 98

Keywords: Arsenic, Mungbean, Growth parameters, Photosynthetic pigments, Chlorophyll a fluorescence


Mungbean is a highly nutritive pulse crop but vulnerable to various abiotic stresses. Arsenic is highly toxic and carcinogenic pollutant of soil and water. Arsenic interferes with several plant metabolic processes reducing plant productivity. In the present study, the consequences of arsenic stress on germination percentage, growth parameters, photosynthetic pigments and photosynthetic parameters have been reported in four mungbean cultivars (IPM02-14, IPM02-03, RMG 975 and Virat). The mungbean cultivars were sown in soil supplemented with arsenic (0, 15, 30 and 45 µM). The decline in the germination percentage, growth parameters, chlorophyll content, photosynthetic parameters and increased activities of antioxidants under arsenic stress was observed in all the cultivars of mungbean. The cultivar variations were also apparent, the cultivar IPM02-14 performed better than IPM02-03, Virat and RMG 975 for all the studied attributes. The enhanced activity of the cultivar IPM02-14 as compared to other cultivars was due to increased activity of various enzymatic and non-enzymatic antioxidants.

Arsenic, Mungbean, Growth parameters, Photosynthetic pigments, Chlorophyll a fluorescence


Ahmad P, Alyemeni MN, Al-Huqail AA, Alqahtani MA, Wijaya L, Ashraf M, Kaya C, Bajguz A (2020) Zinc oxide nanoparticles application alleviates arsenic (As) toxicity in soybean plants by restricting the uptake of As and modulating key biochemical attributes, antioxidant enzymes, ascorbate-glutathione cycle and glyoxalase system. Plants 9(7):825

Alam MZ, McGee R, Hoque M, Ahammed C-B (2019) Effect of arbuscular mycorrhizal fungi, selenium and biochar on photosynthetic pigments and antioxidant enzyme activity under arsenic stress in mung bean (Vigna radiata). Front Physiol 10:193

Ali S, Abbas Z, Rizwan M, Zaheer IE, Yavaş İ, Ünay A, Abdel-Daim MM, Bin-Jumah M, Hasanuzzaman M, Kalderis D (2020) Application of floating aquatic plants in phytoremediation of heavy metals polluted water: a review. Sustainability 12(5):1927

Ando H, Adachi K, Minami M, Nishida N (1988) Effect of soil ammonium nitrogen on the tillering-habit of rice plant. Jpn J Crop Sci 57(4):678–684

Anjum NA, Sharma P, Gill SS, Hasanuzzaman M, Khan EA, Kachhap K, Mohamed AA, Thangavel P, Devi GD, Vasudhevan P, Sofo A (2016) Catalase and ascorbate peroxidase-representative H2O2 detoxifying heme enzymes in plants. Environ Sci Pollut Res 23(19):19002–19029

Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol 24(1):1

Barata RM, Chaparro A, Chabregas SM, González R, Labate CA, Azevedo RA, Sarath G, Lea PJ, Silva-Filho MC (2000) Targeting of the soybean leghaemoglobin to tobacco chloroplasts: effects on aerobic metabolism in transgenic plants. Plant Sci 155(2):193–202

Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207

Das J, Sarkar P (2018) Remediation of arsenic in mung bean (Vigna radiata) with growth enhancement by unique arsenic-resistant bacterium Acinetobacter lwoffii. Sci Total Environ 624:1106–1118

Hasanuzzaman M, Hossain MA, Fujita M (2011) Nitric oxide modulates antioxidant defense and the methylglyoxal detoxification system and reduces salinity-induced damage of wheat seedlings. Plant Biotechnol Rep 5(4):353–365

Hodge DM, DeLong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid reactive substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611

Hu Z, Fan J, Xie Y, Amombo E, Liu A, Gitau MM, Khaldun ABM, Chen L, Fu J (2016) Comparative photosynthetic and metabolic analyses reveal mechanism of improved cold stress tolerance in bermuda grass by exogenous melatonin. Plant Physiol Biochem 100:94–104

Kaya C, Okant M, Ugurlar F, Alyemeni MN, Ashraf M, Ahmad P (2019) Melatonin-mediated nitric oxide improves tolerance to cadmium toxicity by reducing oxidative stress in wheat plants. Chemosphere 225:627–663

Klughammer C, Schreiber U (2008) Complementary PS II quantum yields calculated from simple fluorescence parameters measured by PAM fluorometry and the saturation pulse method. PAM Appl Notes 1(2):201–247

Kumari N, Yadav M, Sharma V (2018) Differential response of Brassica juncea cultivars to Al; consequences for chlorophyll a fluorescence, antioxidants and psb A gene. J Plant Interact 13(1):496–505

Leng Y, Li Y, Wen Y, Zhao H, Wang Q, Li SW (2020) Transcriptome analysis provides molecular evidences for growth and adaptation of plant roots in cadmium-contaminated environments. Ecotoxicol Environ Saf 204:111098

Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22(5):867–880

Natasha N, Shahid M, Khalid S, Bibi I, Naeem MA, Niazi NK, Tack FM, Ippolito JA, Rinklebe J (2021) Influence of biochar on trace element uptake, toxicity and detoxification in plants and associated health risks: a critical review. Crit Rev Environ Sci Technol 52(16):2803–2843

Parvez M, Khan O (2019) Parametric simulation of biomass integrated gasification combined cycle (BIGCC) power plant using three different biomass materials. Biomass Convers Biorefin 10(4):803–812

Rafiq M, Shahid M, Abbas G, Shamshad S, Khalid S, Niazi NK, Dumat C (2017) Comparative effect of calcium and EDTA on arsenic uptake and physiological attributes of Pisum sativum. Int J Phytoremediat 19(7):662–669

Raj L, Ide T, Gurkar AU, Foley M, Schenone M, Li X, Tolliday NJ, Golub TR, Carr SA, Shamji AF, Stern AM (2011) Selective killing of cancer cells by a small molecule targeting the stress response to ROS. Nature 475(7355):231–234

Rehman M, Liu L, Bashir S, Saleem MH, Chen C, Peng D, Siddique KH (2019) Influence of rice straw biochar on growth, antioxidant capacity and copper uptake in ramie (Boehmeria nivea L.) grown as forage in aged copper-contaminated soil. Plant Physiol Biochem 138:121–129

Sepehri A, Sarrafzadeh MH (2019) Activity enhancement of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria in activated sludge process: metabolite reduction and CO2 mitigation intensification process. Appl Water Sci 9(5):1–12

Sharma V, Yadav M, Kumari N (2018) Aluminium Fluoride induced changes in chlorophyll a fluorescence, antioxidants and psb A gene expression of Brassica juncea cultivars. J Plant Interact 13(1):472–548

Siddiqui F, Tandon PK, Srivastava S (2015) Analysis of arsenic induced physiological and biochemical responses in a medicinal plant, Withania Somnifera. Physiol Mol Biol Plant 21(1):61–69

Singh R, Jha AB, Misra AN, Sharma P (2019) Differential responses of growth, photosynthesis, oxidative stress, metals accumulation and NRAMP genes in contrasting Ricinus communis genotypes under arsenic stress. Environ Sci Pollut Res 26(30):31166–31177

Tikkanen M, Grebe S (2018) Switching off photoprotection of photosystem I–a novel tool for gradual PSI photoinhibition. Physiol Plant 162(2):156–161

Zhang H, Sonnewald U (2017) Differences and commonalities of plant responses to single and combined stresses. Plant J 90(5):839–855



The financial support to Sushma Rani provided by the Council of Scientific and Industrial Research (CSIR) New Delhi, India is gratefully acknowledged.

Author Information

Rani Sushma
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk, India