An assessment of pearl millet hybrids for drought tolerance under hyper arid conditions

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-024-00860-0
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Keywords: Antioxidants, Drought, Pearl millet, Relative stress injury, SDS-PAGE


Abstract


An experiment was conducted at Swami Keshwanand Rajasthan Agricultural University, Bikaner (India) on seven hybrids of pearl millet viz. GHB-558, GHB-732, HHB-67 (Improved), HHB-226, MPMH-17, RHB-173 and RHB-177 to find out the drought tolerant pearl millet hybrids for hyper-arid conditions. Experiment was laidout in randomozed block design with three replication. The standard package and practices were applied for all the inputs. However, experiment was maintained as rainfed, without providing any irrigation. Observations on morphological, physio-biochemical and molecular traits were analysed at the time of anthesis using standardized protocols.Yield and yield attributes were measured after harvesting the crop. Results showed that there were significant variations for most of the parameters among seven pearl millet hybrids taken for study. It was observed that the days to 50% flowering, days to maturity and relative stress injury were minimum whereas plant height, biological yield, economical yield, harvest index were maximum in RHB-177 as compared to other hybrids. Similar trends were obtained for total soluble sugars, proline and protein, and antioxidants (superoxide dismutase, catalase and ascorbate peroxidase) activities. The protein profiling pattern of SDS-PAGE also showed the significant variations in these pearl millet hybrids. We found a novel polypeptide band of 55 kDa in RHB-177 under SDS-PAGE, which might be associated with drought tolerance nature. Thus, based on present investigation, it is concluded that pearl millet hybrid RHB-177 is superior drought tolerant owing to existence of drought tolerance mechanism.


Antioxidants, Drought, Pearl millet, Relative stress injury, SDS-PAGE


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Acknowledgements


The experiment was carried out under the project 7CSA-259 of Rastriya Krishi Vikas Yojana (RKVY) and financially supported by the Government of Rajasthan, India (Grant: F/PD/Gr.II/OCS//RKVY/2013-14/753 − 60 dated 06/01/2016).


Author Information


Mamta
Plant Biotechnology Centre, S.K. Rajasthan Agricultural University, Bikaner, India

Agarwal Vishnu Prakash
Plant Biotechnology Centre, S.K. Rajasthan Agricultural University, Bikaner, India
vishnuprakash.agarwal@rediffma

Kumar Ravindra
College of Agriculture, S.K. Rajasthan Agricultural University, Bikaner, India


Chandel Devendra
College of Agriculture, S.K. Rajasthan Agricultural University, Bikaner, India


Gupta Sunita
S.K.N. Agriculture University, Jobner, Jaipur, India

Gupta Narendra Kumar
S.K.N. Agriculture University, Jobner, Jaipur, India
nkgupta.pphy@sknau.ac.in
Sudarsan Yemmanur
Plant Biotechnology Centre, S.K. Rajasthan Agricultural University, Bikaner, India