Anti-oxidant, anti-proliferative activities and characterization of secondary metabolites using Q-TOF-LC-MS/MS analysis of anti-aging medicinal plant Holostemma ada-kodien


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00529-6
First Page: 1285
Last Page: 1294
Views: 1694


Keywords: n Holostemma ada-kodienn , Anti-proliferative MTT assay, Anti-oxidant activity, Phenolic compounds, Flavonoids, Q-TOF-LC-MS/MS


Abstract


Holostemma ada-kodien rhizome is being used for a variety of therapeutic activities especially as a rejuvenative drug for promoting anti-aging properties in traditional Indian medicine. Various in vitro anti-oxidant and anti-proliferative activities of rhizome were analyzed. Detailed LC/MS analysis was done for identifying major chemical constituents. Hydro alcohol extract of H. ada-kodien rhizome showed capable results in DPPH and ABTS scavenging, nitric oxide quenching, reducing power and ferric reducing power antioxidant assays. Extract showed promising anti-proliferative activity in HeLa cell line with an IC50 value 53 µg/ml. In Q-TOF-LC-MS/MS analysis of the hydro alcohol extract, twenty five compounds were identified using their molecular ions and fragmentation pattern. From rhizome, thirteen phenolic acids were identified and most of them were cinnamic acid and benzoic acid derivatives. A coumarin, eleven flavonoids and their glycosides were also identified from the extract. Phenolic acids and their glycosides were found most abundant compounds in extract. Most of the identified compounds were well known antioxidants, and capable of countering reactive free radicals and that might be the reason for rejuvenative and protective behavior of rhizome. The study highlights the unexplored therapeutic activities and major chemical constituents in H. ada-kodien rhizome for the first time.


n              Holostemma ada-kodienn            , Anti-proliferative MTT assay, Anti-oxidant activity, Phenolic compounds, Flavonoids, Q-TOF-LC-MS/MS


*Get Access

(*Only SPR Life Members can get full access.)

Advertisement

References


Chen HJ, Inbaraj BS, Chen BH (2012) Determination of phenolic acids and flavonoids in Taraxacum formosanum Kitam by Liquid Chromatography-Tandem Mass Spectrometry coupled with a post-column derivatization technique. Int J Mol Sci 13:260–285


Chen Y, Yu H, Wu H, Pan Y, Wang K, Jin Y, Zhang C (2015) Characterization and quantification by LC-MS/MS of the Chemical Components of the Heating Products of the Flavonoids Extract in Pollen Typhae for Transformation Rule Exploration. Molecules 20:18352–18366


Chondrogianni N, Kapeta S, Chinou I, Vassilatou K, Papassideri I, Gonos ES (2010) Anti-ageing and rejuvenating effects of quercetin. Exp Gerontol 45:763–771


Deepak M, Sulaiman CT, Balachandran I, Subhashchandran KP (2019) Characterization of Terpenoids Phenolic and Coumarin Compounds from indian Medicinal Plant Holostemma ada kodien and its antioxidant activity. Anal Chem Lett 9:853–862


El-Sayed MA, Al-Gendy AA, Hamdan DI, El-Shazly AM (2017) Phytoconstituents, LC-ESI-MS Profile, antioxidant and antimicrobial activities of Citrus x limon L. Burm. f. Cultivar Variegated Pink Lemon. J Pharm Sci Res 9:375–391


Fan X, Fan Z, Yang Z, Huang T, Tong Y, Yang D, Mao X, Yang M (2022) Flavonoids—natural gifts to Promote Health and Longevity. Int J Mol Sci 23:2176


Feelisch M, Noack E (1987) Nitric oxide formation from nitro vasodilators occur independently of hemoglobin or non-hem iron. Eur J Pharmacol 142:465–469


Gates PJ, Lopes NP (2012) Characterisation of flavonoid aglycones by negative ion chip-based nanospray tandem mass spectrometry. Int J Anal Chem 1:1–7


Girsang E, Lister NE, Ginting CN, Sholihah IA, Raif MA, Kunardi S, Million H, Widowati W (2020) Antioxidant and antiaging activity of rutin and caffeic acid. Pharmaciana 10:147–156


Göger F, Köse YB, Göger G, Demirci F (2015) Phytochemical characterization of phenolics by LC-MS/MS and biological evaluation of Ajuga orientalis from Turkey. Bangladesh J Pharmacol 10:639–644


Hwang E, Park SY, Lee HJ, Lee TY, Sun ZW, Yi TH (2014) Gallic acid regulates skin photoaging in UVB-exposed fibroblast and hairless mice. Phytother Res 28:1778–1788


Jung SK, Lee KW, Kim HY, Oh MH, Byun S, Lim SH, Heo YS, Kang NJ, Bode AM, Dong Z, Lee HJ (2010) Myricetin suppresses UVB-induced wrinkle formation and MMP-9 expression by inhibiting Raf. Biochem Pharmacol 79:1455–1461


Karar MGE, Kuhnert N (2015) UPLC-ESI-Q-TOF-MS/MS characterization of phenolics from Crataegus monogyna and Crataegus laevigata (Hawthorn) Leaves, Fruits and their Herbal Derived drops (Crataegutt Tropfen). J Chem Biol Ther 1:2–23


Lee H, Kim K, Oh C, Park CH, Aliya S, Kim HS, Bajpai VK, Huh YS (2021) Antioxidant and anti-aging potential of a peptide formulation (Gal2–Pep) conjugated with gallic acid. RSC Adv 11:29407–29415


Lee H, Vilian ATE, Kim JY, Chun MH, Suh JS, Seo HH, Cho SH, Shin IS, Kim SJ, Park SH, Han YK, Lee JH, Huh YS (2017) Design and development of caffeic acid conjugated with Bombyx mori derived peptide biomaterials for anti-aging skin care applications. RSC Adv 7:30205–30213


Mena P, Calani L, Dall’Asta C, Galaverna G, García-Viguera C, Bruni R, Crozier A, Del Rio D (2012) Rapid and comprehensive evaluation of (poly)phenolic compounds in Pomegranate (Punica granatum L.) juice by UHPLC-MSn. Molecules 17:14821–14840


Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63


Oyaizu M (1986) Studies on products of browning reactions: antioxidant activities of products of browning reaction prepared from glucose amine. Japanese J Nutrition 44:307–315


Ramiah N, Nair GA, Prasad NBR (1981) Chemical constituents of Holostemma annulare K. Sehun. J Sci Res Plant Med 2:76–78


Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorisation assay. Free Radical Bio Med 26:1231–1237


Rolt A, Cox LS (2020) Structural basis of the anti-ageing effects of polyphenolics: mitigation of oxidative stress. BMC Chem 14:50


Saldanha LL, Vilegas W, Dokkedal AL (2013) Characterization of flavonoids and phenolic acids in Myrcia bella cambess. Using FIA-ESI-IT-MSn and HPLC-PAD-ESI-IT-MS combined with. NMR Molecules 18:8402–8416


Sheng X, Zhu Y, Zhou J, Yan L, Du G, Liu Z, Chen H (2021) Antioxidant Effects of Caffeic Acid lead to Protection of Drosophila Intestinal Stem Cell Aging. Front Cell Dev Biol 9:735483


Silva NA, Rodrigues E, Mercadante AZ, Rosso VV (2014) Phenolic Compounds and Carotenoids from four fruits native from the brazilian Atlantic Forest. J Agric Food Chem 62:5072–5084


Simirgiotis MJ, Benites J, Areche C, Sepúlveda B (2015) Antioxidant capacities and analysis of Phenolic Compounds in three endemic Nolana Species by HPLC-PDA-ESI-MS. Molecules 20:11490–11507


Sulaiman CT, Balachandran I (2012) Total phenolics and total flavonoids in selected indian Medicinal plants. Ind J Pharmaceut Sci 74:258–260


Sun J, Liang F, Bin Y, Li P, Duan C (2007) Screening non-colored phenolics in red wines using liquid chromatography/ultraviolet and mass spectrometry/mass spectrometry libraries. Molecules 12:679–693


Sunil J, Janapati YK, Bramhachari PV (2017) Bioassay guided isolation and identification of the antioxidant constituent from Holostemma ada-kodien shcult. Int J Pharm Bio Sci 8:1–10


Tepe B, Daferera D, Sokmen A, Sokmen M, Polissiou M (2005) Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae). Food Chem 90:333–340


Udayan PS, Balachandran I (2011) Medicinal plants of Arya Vaidya Sala Herb Garden. Arya Vaidya Sala book series, India


Vasu SM (2017) Botanical Pharmacognosy of Holostemma ada-kodien Schult. Pharmacogn J 9:163–170


Wang S, Liu L, Wang L, Hu Y, Zhang W, Liu R (2012) Structural characterization and identification of major constituents in Jitai Tablets by high-performance liquid Chromatography/Diode-Array detection coupled with Electrospray Ionization Tandem Mass Spectrometry. Molecules 7:10470–10493


Wang Y, Catana F, Yang Y, Roderick R, Richard B (2002) An LC-MS method for analyzing total resveratrol in grape juice, cranberry juice, and in wine. J Agric Food Chem 50:431–435


Wu YZ, Tsai YY, Chang LS, Chen YJ (2021) Evaluation of gallic acid-coated gold nanoparticles as an anti-aging ingredient. Pharmaceuticals 14:1071


Wyrepkowski CC, Gomes da Costa DLM, Sinhorin AP, Vilegas W, De Grandis RA, Resende FA, Varanda EA, Dos Santos LC (2014) Characterization and quantification of the Compounds of the Ethanolic Extract from Caesalpinia ferrea stem bark and evaluation of their mutagenic activity. Molecules 19:16039–16057

 


Acknowledgements


The authors are thankful Research and Development Centre, Bharathiar University, Coimbatore and Centre for Medicinal Plants Research, Arya Vaidya Sala, Kottakkal for providing the facility for the successful completion of this work.


Author Information


Deepak M
Research & Development Centre, Bharathiar University, Coimbatore, India
deepakdnivas@gmail.com