Antidiabetic-relevant and differential Nrf2-modulatory activities of Uvaria valderramensis leaf extracts

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E-ISSN: 2229-4473.
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DOI: 10.1007/s42535-026-01869-3
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Keywords: n Uvaria valderramensisn , Antidiabetic, α-glucosidase, α-amylase, Antiglycation, Nrf2


Abstract


Uvaria valderramensis, an endemic Philippine species, has not previously been evaluated for antidiabetic activity. This study evaluated the antidiabetic-relevant and Nrf2-modulatory activities of its leaf extracts using in vitro assays for α-glucosidase and α-amylase inhibition, antiglycation activity, and Nrf2 reporter modulation. Petroleum ether (UvP), dichloromethane (UvD), and methanol (UvM) extracts were obtained by cold percolation. UvM exhibited significant α-glucosidase inhibition (IC50 = 44.21 µg/mL), whereas none of the extracts inhibited α-amylase. UvP demonstrated moderate inhibition of advanced glycation end-product formation (IC50 = 333.6 µg/mL). In luciferase reporter assays, UvP increased Nrf2 activity in HaCaT keratinocytes (111.22% vs. DMSO control; p < 0.05), while UvP and UvM reduced Nrf2 activity in Huh7 hepatocellular carcinoma cells (24.7% and 67.6%, respectively; p < 0.05). The extracts also did not promote cytotoxicity. These findings demonstrate extract-specific bioactivities involving carbohydrate-digestive enzyme inhibition, antiglycation activity, and context-dependent Nrf2 modulation. This study provides the first in vitro evidence of antidiabetic-relevant and Nrf2-modulatory bioactivities of U. valderramensis leaf extracts and supports further chemical, mechanistic, cellular, and in vivo investigations.

n                     Uvaria valderramensisn                  , Antidiabetic, α-glucosidase, α-amylase, Antiglycation, Nrf2


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Author Information


Department of Biological Sciences, College of Science, University of Santo Tomas, Manila, Philippines