Antimicrobial activity and mycochemical profile of methanol extract from Pleurotus flabellatus

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-021-00242-w
First Page: 619
Last Page: 629
Views: 765

Keywords: Antimicrobial, Mycochemical, Pathogens, Extract, Methanol


Pleurotus flabellatus is an edible mushroom possessing high nutritive as well as medicinal values. This study specifically investigated the antimicrobial properties of the methanol extract of P. flabellatus. Agar well diffusion and INT colorimetric assay methodologies were used to assess the antimicrobial activity of the extract against selected test pathogenic microorganisms viz. Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris NCIM 5266, Shigella flexeneri NCIM 5265, Proteus mirabilis NCIM 2300, Serratia marcescens NCIM 2078 and Moraxella catarrhalis NCIM 2795. Preliminary mycochemical screening revealed the presence of secondary metabolites such as flavonoids, diterpenoids, triterpenoids, alkaloids, phenols, carbohydrates, proteins, aminoacids, phlobatamins, terpenes and saponins, etc. which is responsible for antimicrobial activity. The thin-layer chromatography (TLC) eluted out 5 different compounds from the spot (extract) with solvent system chloroform + methanol (9.5:0.5) when visualized in UV, Iodine chamber and with sprayed acidified vanillin solution (0.1 g vanillin:28 ml methanol:1 ml sulphuric acid). The extract showed a mean zone of growth inhibition of 10.3 ± 0.3 to 17.6 ± 0.3 mm against the test pathogens. The methanol extracts showed bactericidal activity against all the test pathogens at MIC values of 26.03–83.33 mg/ml. Whereas the MBC values for methanol extract of P. flabellatus against all pathogens were recorded as 62.5–104.16 mg/ml. S. aureus which was resistant to commercial antibiotic penicillin gave highest antimicrobial activity (17.6 ± 0.3 mm) for methanol extract in comparison to other test pathogens. Serratia marcescens showed lowest antimicrobial activity with zone of inhibition 10.3 ± 0.3 mm. Among all the test pathogens, due to low MIC value of Proteus mirabilis, it showed higher total antibacterial Activity (TAA). Methanol extracts of P. flabellatus exhibited broad-spectrum of antimicrobial activity against test pathogens probably due to presence of several bioactive compounds that may serve as potential antimicrobial agents.

Antimicrobial, Mycochemical, Pathogens, Extract, Methanol

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The authors thank the University of Allahabad for providing the necessary funding in the form of UGC-CRET fellowship and institutional facilities.

Author Information

Pandey Aprajita Tiwari
Centre of Biotechnology, Nehru Science Complex, University of Allahabad, Prayagraj, India
Pandey Ishan
Department of Pathology, Motilal Nehru Medical College, Prayagraj, India

Kerkar Pranali
I.C.M.R.-National AIDS Research Institute, Pune, India
Singh M. P.
Centre of Biotechnology, Nehru Science Complex, University of Allahabad, Prayagraj, India