Assessment of antibacterial activities of mycelium and exopolysaccharide extract of two different Lentinus species collected from Tripura

*Article not assigned to an issue yet

, , ,


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-023-00726-x
First Page: 0
Last Page: 0
Views: 1243


Keywords: Antibacterial activity, n Lentinus speciesn , Mycelial extract, Exopolysaccharide extract, Pathogenic bacteria


Abstract


For the past 20 years, different biologically active compounds isolated from mushrooms have been playing a crucial role in numerous aspects of natural medicine development. These bioactive compounds are believed to have a large number of potential sources of antibacterial attributes. The present investigation has been done to determine the antibacterial activities of exopolysaccharide and mycelia extract of two different Lentinus species (MCCT L1 and MCCT P7) collected from Tripura. In-vitro Antibacterial effectivity of exopolysaccharide and mycelial extracts of two different mushrooms was determined against four pathogenic bacteria. In this present study, it has been found that the mycelial extract had better inhibitory activity on the growth of gram-positive bacteria than the gram-negative bacteria. Whereas, in the case of gram-negative the exopolysaccharide extract of two different experimental samples showed higher inhibitory activity than the gram-positive bacteria. Among the two different selected mushroom samples (MCCT L1 and MCCT P7), MCCT P7 was found more effective than MCCT L1 to suppress the activity of both gram-positive and gram-negative bacteria.


Antibacterial activity, n              Lentinus speciesn            , Mycelial extract, Exopolysaccharide extract, Pathogenic bacteria


*Get Access

(*Only SPR Members can get full access. Click Here to Apply and get access)

Advertisement

References


Agrahar-Murugkar D, Subbulakshmi G (2004) Nutritional value of edible wild mushrooms collected from the Khasi hills Meghalaya. Food Chem 89:599–603. https://doi.org/10.1016/j.foodchem.2004.03.042


Akyuz M, Kirbag S (2009) Antimicrobial activity of Pleurotus eryngii var. Ferulae grown on various agro-wastes. Eurasian J Biosci 3:58–63. https://doi.org/10.5053/ejobios.2009.3.08


Attarat J, Thamisak R (2014) Anticancer PSP and phenolic compounds in Lentinus squarrosulus and Lentinus polychrous. The 5th International Conference on Natural Products for Health and Beauty 263–267


Bajpai K, Majumder V, Rather IA, Kim K (2016) Extraction, isolation and purification of exopolysaccharide from lactic acid bacteria using ethanol precipitation method. Bangladesh J Pharmacol 11:573–576. https://doi.org/10.3329/bjp.v11i3.27170


Breene WM (1990) Nutritional and medical value of specialty mushrooms. J Food Prot. https://doi.org/10.4315/0362-028x-53.10.883. 53:883 – 94


Benedict RG, Brady LR (1972) Antimicrobial activity of mushroom metabolites. J Pharm Sci 61:1820–1822. https://doi.org/10.1002/jps.2600611130


Boonsong S, Klaypradit W, Wilaipun P (2016) Antioxidant activities of extracts from five edible mushrooms using different extractants. Agric Nat Resour 50:89–97. https://doi.org/10.1016/j.anres.2015.07.002


Burnett LC, Lunn G, Coico R (2009) Biosafety: guidelines for working with pathogenic and infectious microorganisms. Curr Protoc Microbiol 13(1):111–1114. https://doi.org/10.1002/9780471729259.mc01a01s13





Choi Y, Lee SM, Chun J, Lee HB, Lee J (2006) Influence of heat treatment on the antioxidant activities and polyphenolic compounds of shiitake (Lentinula edodes) mushroom. Food Chem 99(2):381–387. https://doi.org/10.1016/j.foodchem.2005.08.004


Collins CH, Lyne PM (1987) Microbiological methods’’. Butter Worths and Co (Publishers) Ltd., London


Das SK, Manda A, Datta AK, Gupta S, Paul R (2013) Nucleotide sequencing and identification of some wild mushrooms. Sci World J 13:1–8. https://doi.org/10.1155/2013/403191


Deveci E, Tel-Cayan G, Duru ME (2021) Inhibitory activities of medicinal mushrooms on α-amylase and α-glucosidase-enzymes related to type 2 diabetes. South Afr J Bot 137:19–23. https://doi.org/10.1080/10942912.2018.1431660


Duru ME, Tel-Cayan G (2015) Biologically active terpenoids from mushroom origin: a review. Records of Natural Products 9:456–483. https://www.researchgate.net/publication/280153112


Enman J, Rova U, Berglund KA (2007) Quantification of the bioactive compound eritadenine in selected strains of shiitake mushroom (Lentinula edodes). J Agric Food Chem 55(4):1177–1180. https://doi.org/10.1021/jf062559+


Erdogan Eliuz EA (2020) Antimicrobial activity of citric acid against Escherichia coli, Staphylococcus aureus and Candida albicans as a sanitizer agent. Eurasian J for Sci 8(3):295–301. https://doi.org/10.3195/ejejfs.787021


Hassegawa RH, Kasuya MCM, Vanetti MCD (2005) Growth and antibacterial activity of Lentinula edodes in liquid media supplemented with agricultural wastes. Electron J Biotechnol 8(2):212–217. https://doi.org/10.2225/vol8-issue2-fulltext-3


Hatvani N (2001) Antibacterial effect of the culture fluid of Lentinus edodes mycelium grown in submerged liquid culture. Int J Antimicrob Agents 17:71–74. https://doi.org/10.1016/s0924-8579(00)00311-3


Hearst R, Nelson D, McCollum G, Millar B, Maeda Y et al (2009) An examination of antibacterial and antifungal properties of constituents of shiitake (Lentinula edodes) and oyster (Pleurotus ostreatus) mushrooms. Complement Ther Med 15(1):5–7. https://doi.org/10.1016/j.ctcp.2008.10.002


Herrmann H (1962) Cortinellin, eine antibiotisch wirksame substanz aus cortinellus shiitake. Die Naturwiss 49:542


Hirasawa M, Shouji N, Neta T (1999) Three kinds of antibacterial substances from Lentinus edodes (Berk.) Sing. (shiitake, an edible mushroom). Int J Antimicrob Agents 11:151–157. https://doi.org/10.1016/s0924-8579(98)00084-3


Iwalokun BA, Usen UA, Otunba AA, Olukoya DK (2007) Comparative phytochemical evaluation, antimicrobial and antioxidant properties of Pleurotus ostreatus. Afr J Biotechnol 6:1732–1739. https://doi.org/10.5897/AJB2007.000-2254


Ishikawa NK, Kasuya MCM, Vanetti MCD (2001) Antibacterial activity of Lentinula edodes grown in liquid medium. Brazilian J Microbiol 32(3):206–210. https://doi.org/10.1590/S1517-83822001000300008


Kim MY, Park MH, Kim GH (1997) Effects of mushroom protein-bound polysaccharides on the blood glucose levels and energy metabolism in streptozotocin induced diabetic rats. Korean J Nutr 30:743–750


Komemushi S, Yamamoto Y, Fujita T (1995) Antimicrobial substance by Lentinus edodes. J Antibact Antifungal Agents 23:81–86


Komemushi S, Yamamoto Y, Fujita T (1996) Purification and identification of antimicrobial substances produced by Lentinus edodes. J Antibact Antifungal Agents 24(1):21–25


Lindequist U, Niedermeyer THJ, Julich WD (2005) The pharmacological potential of mushrooms. Evidence-Based Complement Altern Med 2:285–299. https://doi.org/10.1093/ecam/neh107


Maeda YY, Takahama S, Yonekawa H (1998) Four dominant loci for the vascular responses by the antitumor polysaccharide, lentinan. Immunogenetics 47:159–165. https://doi.org/10.1007/s002510050341


Majolagbe ON, Oloke JK, Adebayo EA, Adewoyin AG, Ayandele A et al (2013) Study on the antibacterial activity of Exopolysaccharides of Lentinus subnudus using swiss albino rats as animal model. American-Eurasian J Sci Res 8(1):47–52. https://doi.org/10.5829/idosi.aejsr.2013.8.1.66211


Mau LJ, Huang PN, Hung SJ, Chen CC (2004) Antioxidant property of methanolic extract from two kinds of Antrodia camphorates mycelia. Food Chem 86:25–31. https://doi.org/10.1016/j.foodchem.2003.08.025


Ngai PHK, Ng TB (2003) Lentin, a novel and potent antifungal protein from shitake mushroom with inhibitory effects on activity of human immunodeficiency virus-1 reverse transcriptase and proliferation of leukemia cells. Life Sci 73:3363–3374. https://doi.org/10.1016/j.lfs.2003.06.023


Obodai M, Ferreira ICFR, Fernandes A, Barros L, Mensah DLN, Dzomeku M, Urben AF, Prempeh J, Takli RK (2014) Evaluation of the chemical and antioxidant properties of wild and cultivated mushrooms of Ghana. Molecules 19:19532–19548. https://doi.org/10.3390/molecules191219532


Pegler DN (1977) A preliminary agaric flora of East Africa. Kew Bull Additional Ser Ser 6:15–571


Purkayastha RP, Chandra A (1985) Manual of indian edible mushrooms. Today and Tomorrow’s Printers and Publishers, New Delhi, pp 192–194


Ramos M, Burgos N, Bernard A, Evans G, Preece J et al (2019) Agaricus bisporus and its by-products a source of valuable extracts and bioactive compounds. Food Chem 292:176–187. https://doi.org/10.1016/j.foodchem.2019.04.035


Rao JR, Smyth TJ, Millar BC, Moore JE (2009) Antimicrobial properties of shiitake mushrooms (Lentinula edodes). Int J Antimicrob Agents 33(6):591–592. https://doi.org/10.1016/j.ijantimicag.2008.10.018


Rathee S, Rathee D, Kumar V, Rathee P (2011) Mushrooms as therapeutic agents. J Pharmacognosy Phytotherapy 22:459–474. https://doi.org/10.1590/S0102-695X2011005000195


Rogers JD, Ju YM, Lehmann J (2005) Some Xylaria species on termite nests. Mycologia 97(4):914–923. https://doi.org/10.1080/15572536.2006.11832783


Sanchez-Minutti L, Tellez-Tellez M, Tlecuitl-Beristain S, Santos-Lopez G, Diaz R et al (2016) Antimicrobial activity of a protein obtained from fruiting body of Lentinula edodes against Escherichia coli and Staphylococcus aureus. J Environ Biol 37:619–623


Sugiyama K, Akachi T, Yamakawa A (1995) Hypocholesterolemic action of eritadenine is mediated by a modification of hepatic phospholipid metabolism in rats. J Nutr 125:2134–2144. https://doi.org/10.1093/jn/125.8.2134


Sullivan R, Smith JE, Rowan NJ (2006) Medicinal mushrooms and cancer therapy: translating a traditional practice into western medicine. Perspect Biol Med 49(2):159–170. https://doi.org/10.1353/pbm.2006.0034


Tabbouche SA, Gurgen A, Yildiz S (2017) Antimicrobial and anti-Quorum sensing activity of some wild mushrooms collected from Turkey. J Sci Technol MSU 5(2):453–457. http://dergipark.gov.tr/download/article-file/386198


Ubaidillah NHN, Abdullah N, Sabaratnam V (2015) Isolation of the intracellular and extracellular polysaccharides of Ganoderma neojaponicum (Imazeki) and characterization of their immunomodulatory properties. Electron J Biotechnol 18:188–195. https://doi.org/10.1016/j.ejbt.2015.03.006


Wandati TW, Kenji GM, Onguso JM (2013) Phytochemicals in Edible Wild Mushrooms from selected Areas in Kenya. J Food Res (JFR) 2(3):137–144. https://doi.org/10.5539/jfr.v2n3p137


Wasser SP, Weis AL (1999) Therapeutic effects of substances occurring in higher Basidiomycetes mushrooms: a modern perspective. Crit Rev Immunol 19:65–96. https://doi.org/10.1615/INTJMEDMUSHROOMS.V1.I1.30


Woldegiorgis AZ, Abate D, Haki GD, Ziegler GR (2014) Antioxidant property of edible mushrooms collected from Ethiopia. Food Chem 57:30–36. https://doi.org/10.1016/j.foodchem.2014.02.014


Yang BK, Kim DH, Jeong SC, Das S, Chaoi YS et al (2002) Hypoglycemic effect of a Lentinus edodes exo-polymer produced from a submerged mycelial culture. Bioscience Biotechnol Biochem 66(5):937–942. https://doi.org/10.1271/bbb.66.937

 


Acknowledgements


The first author is grateful to the UGC, Government of India for providing non-NET fellowship and Head of the Botany Department for providing Laboratory facilities.


Author Information


Dutta Atrayee
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Tripura, India
atrayeebot@gmail.com
Mahananda Anuradha
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Tripura, India


Adhikari Supriya
Cytogenetics and Plant Biotechnology Laboratory, Department of Botany, Tripura University, Tripura, India


Saha Ajay Krishna
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Tripura, India