Assessment of genetic diversity in Tecomella undulata by using ISSR markers

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-023-00571-y
First Page: 1526
Last Page: 1534
Views: 1400


Keywords: DNA, Amplification, Base pairs, Polymorphism and genetic variation


Abstract


The present study was carried out to assess the genetic diversity among 24 genotypes of Tecomella undulata by using of inter simple sequence repeats (ISSR) markers. Leaves samples were used as the primary material for DNA extraction. The DNA from collected plants were screened for genetic diversity using 40 ISSR primers. The number of countable bands for each ISSR primer varied from 4 to 7. Thirteen ISSR primers produced 67 distinct bands, 42 bands were polymorphic while 25 were monomorphic. Overall size of PCR amplified products was ranged between 120 to 1450 bps. The percentage of polymorphism was also calculated for each primer and the maximum polymorphism was observed for primer ISSR 35 (85.71%) while minimum polymorphism was observed with primer ISSR 31 (40.00%). Average 62.36% polymorphism was detected amongst 24 genotypes of T. undulata using 13 ISSR primers. Genetic variation between all the genotypes using ISSR markers suggested that these markers are efficient tools for observing genetic relationship in particular tree species which can be utilized for breeding programs of T. undulata for developing new varieties.


DNA, Amplification, Base pairs, Polymorphism and genetic variation


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Acknowledgements



Author Information


Daneva Vijay
Department of Forestry, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India
danevavijay.2000@gmail.com
Beniwal R. S.
Department of Forestry, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India


Kajla Subhash
Centre for Plant Biotechnology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India


Poonia Anil Kumar
Centre for Plant Biotechnology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India


Kumar Manish
Department of Forestry, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India

Kajal
Department of Forestry, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India