Bio-nematicidal activities by culture filtrate of Bacillus subtilis HussainT-AMU: new promising biosurfactant bioagent for the management of Root Galling caused by Meloidogyne incognita

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-020-00099-5
First Page: 229
Last Page: 238
Views: 1082

Keywords: Biocontrol, Biosurfactant, Root knot nematode, Bacillus subtilis


Root galling caused by Meloidogyne incognita causes dramatic yield reduction in a wide range of crops, especially vegetables, at most 15–25%, however, in severe cases it can reach upto 75%. A new biosurfactant bacteria characterized through 16S rRNA gene sequencing and biochemically as Bacillus subtilis HussainT-AMU that was isolated from oil contaminated soil from Odisha coat line near Paradip port exhibiting significant biosurfactant activity in all screening assays. Most of the in vitro experiments were carried out at room temperature. Exposure of fresh culture filtrate from new bioagent to M. incognita caused substantial mortality of the second stage juvenile (J2) and significantly reduced egg hatchability. 100% of culture filtrate collected from B. subtilis HussainT-AMU caused J2 mortality and reduced hatching (p < 0.05). Hatching was defective by 97–99%. More than 90% J2 mortality occurred after 24 h of exposure in 100%. Significant reduction (58.79%) in number of galls/plant in tomato roots in combined application leading to reduction in root galling was recorded. This novel biosurfactant bacterial strain seems to be a safe and effective bio-nematicide potential that needs to be explored more both at field study as well genomic level.

Biocontrol, Biosurfactant, Root knot nematode, 
                Bacillus subtilis

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Authors are thankful to the Department of Botany, Aligarh Muslim University, Aligarh, India for providing infrastructure support and University Sophisticated Instruments Facility (USIF), AMU, Aligarh for SEM facility.

Author Information

Hussain Touseef
Plant Pathology and Nematology Section, Department of Botany, Aligarh Muslim University, Aligarh, India
Haris Mohammad
Plant Pathology and Nematology Section, Department of Botany, Aligarh Muslim University, Aligarh, India

Shakeel Adnan
Plant Pathology and Nematology Section, Department of Botany, Aligarh Muslim University, Aligarh, India

Ahmad Gufran
Plant Pathology and Nematology Section, Department of Botany, Aligarh Muslim University, Aligarh, India

Ahmad Khan Abrar
Plant Pathology and Nematology Section, Department of Botany, Aligarh Muslim University, Aligarh, India

Khan Mohd. A.
Div. of Soil Science and Agricultural Chemicals, ICAR-Indian Agricultural Research Institute, New Delhi, India