Biosynthesis of silver nanoparticles using the endophyte Enterobacter roggenkampii BLS02 from Barleria lupulina and their role in the inhibition of food borne bacteria


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00514-z
First Page: 1245
Last Page: 1255
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Keywords: Silver nanoparticles, n Enterobacter roggenkampii BLS02, n Barleria lupulinan , Antibacterial activity, Food-borne bacteria


Abstract


This study was aimed to synthesize silver nanoparticles using an endophytic bacterium Enterobacter roggenkampii BLS02, which was isolated from a medicinal plant Barleria lupulina. Synthesis of silver nanoparticles was confirmed visually by the silver-impregnated bacterial culture changing color from transparent white to dark brown. The morphology of the silver nanoparticles and the size of 20-80 nm were studied through UV-visible spectroscopy, Fourier-transformed infrared spectroscopy (FTIR), X-ray diffraction, field emission scanning electron microscopy with energy dispersive X-ray analysis (EDX), and transmission electron microscope. The role of secondary metabolites in the reduction, stabilization, and capping of silver nanoparticles was studied using qualitative FTIR spectral peaks. The growth of two food-borne bacteria, Pseudomonas aeruginosa and Listeria monocytogenes treated with biosynthesized silver nanoparticles at 30 μg/mL were strongly inhibited showing a strong antibacterial effect of silver nanoparticles against food-borne bacteria. Thus, the silver nanoparticles proved to have the potential to protect food from food-borne bacteria.

Graphical abstract


Silver nanoparticles, n                     Enterobacter roggenkampii BLS02, n              Barleria lupulinan            , Antibacterial activity, Food-borne bacteria


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References


Acharya D, Singha KM, Pandey P, Mohanta B, Rajkumari J, Singha LP (2018) Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria. Sci Rep. https://doi.org/10.1038/s41598-017-18590-6


Ahmed T, Shahid M, Noman M, Niazi MBK, Mahmood F, Manzoor I, Chen J (2020) Silver nanoparticles synthesized by using Bacillus cereus SZT1 ameliorated the damage of bacterial leaf blight pathogen in rice. Pathogens. https://doi.org/10.3390/pathogens9030160


Al-Mutabagani LA, Alshabanah LA, Ahmed HA, Alalawy HH (2021) Synthesis, mesomorphic and computational characterizations of nematogenic schiff base derivatives in pure and mixed state. Molecules. https://doi.org/10.3390/molecules26072038


Arshad H, Sami MA, Sadaf S, Hassan U (2021) Salvadorapersica mediated synthesis of silver nanoparticles and their antimicrobial efficacy. Sci Rep. https://doi.org/10.1038/s41598-021-85584-w


Chandrasekharan S, Chinnasamy G, Bhatnagar S (2022) Sustainable phyto-fabrication of silver nanoparticles using Gmelina arborea exhibit antimicrobial and biofilm inhibition activity. Sci Rep. https://doi.org/10.1038/s41598-021-04025-w


Chintagunta AD, Nalluru S (2021) Nanotechnology: an emerging approach to combat COVID-19. Emerg Mater. https://doi.org/10.1007/s42247-021-00178-6


Duhan P, Bansal P, Rani S (2020) Isolation, identification and characterization of endophytic bacteria from medicinal plant Tinosporacordifolia. S Afr J Bot. https://doi.org/10.1016/j.sajb.2020.01.047


Elbahnasawy MA, Shehabeldine AM, Khattab AM, Amin BH, Hashem AH (2021) Green biosynthesis of silver nanoparticles using novel endophytic Rothiaendophytica: characterization and anticandidal activity. J Drug Deliv Sci Technol. https://doi.org/10.1016/j.jddst.2021.102401


Gakuubi MM, Munusamy M, Liang ZX, Ng SB (2021) Fungal endophytes: a promising frontier for discovery of novel bioactive compounds. J Fungi. https://doi.org/10.3390/jof7100786


Gomes HI, Martins CS, Prior JA (2021) Silver nanoparticles as carriers of anticancer drugs for efficient target treatment of cancer cells. Nanomaterials. https://doi.org/10.3390/nano11040964


Hoque MI, Jahan Rima SA, Uddin MS, Julkarnain M (2021) Synthesis and antibacterial activity of silver nanoparticles against Escherichia coli and Pseudomonas sp. Int J Nanosci. https://doi.org/10.1142/S0219581X21500435


Huq M, Akter S (2021) Characterization and genome analysis of Arthrobacter bangladeshi sp. Nov. applied for the green synthesis of silver nanoparticles and their antibacterial efficacy against drug-resistant human pathogens. Pharmaceutics. https://doi.org/10.3390/pharmaceutics13101691


Javed B, Raja NI, Nadhman A (2020) Understanding the potential of bio-fabricated non-oxidative silver nanoparticles to eradicate Leishmania and plant bacterial pathogens. Appl Nanosci. https://doi.org/10.1007/s13204-020-01355-5


Khan MS, Gao J, Chen X, Zhang M, Yang F, Du Y, Zhang X (2020) Isolation and characterization of plant growth-promoting endophytic bacteria Paenibacilluspolymyxa SK1 from Liliumlancifolium. Biomed Res Int. https://doi.org/10.1155/2020/8650957


Kourmouli A, Valenti M, van Rijn E, Beaumont HJ, Kalantzi OI, Schmidt-Ott A, Biskos G (2018) Can disc diffusion susceptibility tests assess the antimicrobial activity of engineered nanoparticles? J Nanopart Res. https://doi.org/10.1007/s11051-018-4152-3


KthiriA HS, Othmani A, Landoulsi A, O’Sullivan S, Sheehan D (2021) Novel static magnetic field effects on green chemistry biosynthesis of silver nanoparticles in Saccharomyces cerevisiae. Sci Rep. https://doi.org/10.1038/s41598-021-99487-3


Kumar N, Dubey RC (2022) Plant growth-promoting attributes of an endophyte Enterobacter roggenkampii BLS02 isolated from Barleria lupulina Lindl. Org Agric. https://doi.org/10.1007/s13165-021-00375-x


Kumar S, Stecher G, Li M, Knyaz Cand Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. https://doi.org/10.1093/molbev/msy096


Kumari R, Kumar S, Kumar A, Goel KK, Dubey RC (2017) Antibacterial, antioxidant and Immuno-modulatory properties in extracts of Barleria lupulina Lindl. BMC Complement Altern Med. https://doi.org/10.1186/s12906-017-1989-4


Momzyakova KS, Valishina ZT, Deberdeev TR, Aleksandrov AA, BerlinDeberdeev AARY (2021) Structural analysis of powder celluloses by FTIR spectroscopy. Polym Sci Ser. https://doi.org/10.1134/S1995421221020222


Ocwieja M, Barbasz A (2020) Sodium hexametaphosphate–induced enhancement of silver nanoparticle toxicity towards leukemia cells. J Nanopart Res. https://doi.org/10.1007/s11051-020-04903-w


Pareek V, Devineau S, Sivasankaran SK, Bhargava A, Panwar J, Srikumar S, Fanning S (2021) Silver nanoparticles induce a triclosan-like antibacterial action mechanism in multi-drug resistant Klebsiella pneumoniae. Front Microbiol. https://doi.org/10.3389/fmicb.2021.638640


Singh S, Hiranmai RY (2021) Monitoring and molecular characterization of bacterial species in heavy metals contaminated roadside soil of selected region along NH 8A, Gujarat. Heliyon. https://doi.org/10.1016/j.heliyon.2021.e08284


Scotti F, Mou L, Huang C, Booker A, Weckerle C, Maake Cand Heinrich M (2021) Treating chronic wounds using photoactive metabolites: data mining the Chinese pharmacopoeia for potential lead species. Planta Med. https://doi.org/10.1055/a-1578-8778


Shao Y, Wu C, Wu T, Yuan C, Chen S, Ding Tand HuY (2018) Green synthesis of sodium alginate-silver nanoparticles and their antibacterial activity. Int J Biol Macromol. https://doi.org/10.1016/j.ijbiomac.2018.01.012


Singh R (2021) Green synthesis of silver nanoparticles using methanol extract of Ipomoea carnea Jacq. to combat multidrug resistance bacterial pathogens. Curr Res Green Sustain Chem. https://doi.org/10.1016/j.crgsc.2021.100152


Sinha S, Singh RK, Kumar N, Singh SP, Dwivedi PK, Kumari R (2021) Preparation and exploration of physical properties of calcium based Indian origin ayurvedic medicine ShankhBhasma (marine drug) as nanomaterials for its applications. J Nat Remed. https://doi.org/10.18311/jnr/2021/26225


Sudarsan S, Kumar Shankar M, Kumar BelagalMotatis A, Shankar S, Krishnappa D, Mohan CD, Siddaiah CN (2021) Green synthesis of silver nanoparticles by Cytobacillus firmus isolated from the stem bark of Terminalia arjuna and their antimicrobial activity. Biomolecules. https://doi.org/10.3390/biom11020259


Talley J, Davis LB, Morin Band Liu L (2021) Quantifying the impact of consumer behavior on foodborne illness using a compartment model. Comput Ind Eng. https://doi.org/10.1016/j.cie.2020.106923


Uznanski P, Zakrzewska J, Favier F, Kazmierski Sand Bryszewska E (2017) Synthesis and characterization of silver nanoparticles from (bis) alkylamine silver carboxylate precursors. J Nanopart Res. https://doi.org/10.1007/s11051-017-3827-5


Wang Z, Yu ZX, Solanki MK, Yang LT, Xing YX, Dong DF, Li YR (2020) Diversity of sugarcane root-associated endophytic Bacillus and their activities in enhancing plant growth. J Apple Microbiol. https://doi.org/10.1111/jam.14512


Yesiltas M, Glotch TD, Sava B (2021) Nano-FTIR spectroscopic identification of prebiotic carbonyl compounds in Dominion Range 08006 carbonaceous chondrite. Sci Rep 11:1–9. https://doi.org/10.1038/s41598-021-91200-8


Yuan W, Zhu B, Li XY, Hansen TW, Ou Y, Fang K, Wang Y (2020) Visualizing H2O molecules reacting at TiO2 active sites with transmission electron microscopy. Sci. https://doi.org/10.1126/science.aay2474


Zhang J, Guo Y, Pau D, Li K, Xie K, Zou Y (2021) Pyrolysis kinetics and determination of organic components and N-alkanes yields of Karamay transformer oil using TG, FTIR and Py-GC/MS analyses. Fuel. https://doi.org/10.1016/j.fuel.2021.121691

 


Acknowledgements


The authors would like to express their gratitude to the Department of Botany and Microbiology for providing them with the chance to conduct this research.


Author Information


Kumar Nikhil
Department of Botany and Microbiology, Gurukul Kangri (Deemed to be University), Haridwar, India
kumarnikhil.mb019@gmail.com