Biosynthesis of silver nanoparticles using the endophyte Enterobacter roggenkampii BLS02 from Barleria lupulina and their role in the inhibition of food borne bacteria

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-022-00514-z
First Page: 1245
Last Page: 1255
Views: 1631

Keywords: Silver nanoparticles, n Enterobacter roggenkampii BLS02, n Barleria lupulinan , Antibacterial activity, Food-borne bacteria


This study was aimed to synthesize silver nanoparticles using an endophytic bacterium Enterobacter roggenkampii BLS02, which was isolated from a medicinal plant Barleria lupulina. Synthesis of silver nanoparticles was confirmed visually by the silver-impregnated bacterial culture changing color from transparent white to dark brown. The morphology of the silver nanoparticles and the size of 20-80 nm were studied through UV-visible spectroscopy, Fourier-transformed infrared spectroscopy (FTIR), X-ray diffraction, field emission scanning electron microscopy with energy dispersive X-ray analysis (EDX), and transmission electron microscope. The role of secondary metabolites in the reduction, stabilization, and capping of silver nanoparticles was studied using qualitative FTIR spectral peaks. The growth of two food-borne bacteria, Pseudomonas aeruginosa and Listeria monocytogenes treated with biosynthesized silver nanoparticles at 30 μg/mL were strongly inhibited showing a strong antibacterial effect of silver nanoparticles against food-borne bacteria. Thus, the silver nanoparticles proved to have the potential to protect food from food-borne bacteria.

Graphical abstract

Silver nanoparticles, n                     Enterobacter roggenkampii BLS02, n              Barleria lupulinan            , Antibacterial activity, Food-borne bacteria

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The authors would like to express their gratitude to the Department of Botany and Microbiology for providing them with the chance to conduct this research.

Author Information

Kumar Nikhil
Department of Botany and Microbiology, Gurukul Kangri (Deemed to be University), Haridwar, India