Changes in morpho-physiological attributes in nine genotypes of linseed (Linum usitatissimum L.) under different level of salt (NaCl) stress

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-021-00228-8
First Page: 647
Last Page: 653
Views: 623

Keywords: Linseed, Salt Stress, Seed vigour, Antioxidant enzymes


In the era of global climate change agricultural productivity is threatened by array of abiotic stresses with scarcity of irrigation water. Overcoming the deleterious effect of abiotic stressors is most prominent challenge for enhancement of germination, seedling character and physiological analysis. Therefore, the present study was carried out with the aim to evaluate the physiological responses of nine heterosis line of linseed (Linum usitatissimum L.). Seed germinated under four level of salinity stress (100 mM, 150 mM, 200 mM, and 250 mM NaCl) to explore the adaptive mechanisms involved. The germination percentage, root length, shoots length, and seedling vigor index significantly varied under different treatments of salt stress. Markers of oxidative stress, such as peroxidase (pox), superoxide dismutase (SOD), proline (pro) and malondialdehyde (MDA) might be the central components of adaptive mechanism of plants to maintain cellular ionic homeostasis under salt stress. The outcome of this study emphasized that the gradual increase of NaCl decreased all the morphological characteristics except the oxidative stress markers. We found that all the tested genotype showed differential response in terms of proline and MDA content along with peroxidase and SOD, to counteract the salt stress through maintenance of cellular osmotic adjustment. Among all the tested genotypes SHA7, followed by SHA8 and SHA9 performed exceeded record of germination and seedling characteristics through osmotic adjustment by the regulation of ionic homeostasis by oxidative stress markers.

Linseed, Salt Stress, Seed vigour, Antioxidant enzymes

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  2. Abido WAE, Zsombik L (2019) Effect of salinity on germination character and seedlings parameters of Egyptian flax cultivars growing in Nyiregyhaza. Acta Ecologia Sinicia 39:102–108.

  3. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39(1):205–207


  5. Beyer WF, Fridovich I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161:559–566

  6. Bojovic S, Matic R, Popovic Z, Smiljanic M, Vidakovic V (2013) An overview of forestry journals in the period 2006–2010 as basis for ascertaining research trends. Scientometrics 98(2):1331–1346.

  7. Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

  8. De-Lacerda CF, Cambraia J, Oliva MA, Ruiz HA, Prisco JT (2003) Solute accumulation and distribution during shoot and leaf development in two sorghum genotypes under salt stress. Environ Exp Bot 49:107–120.

  9. FAO (2019) Food and agricultural commodities production. http://faostat.fao.GeneticdiversitystudyinIndianandexoticlinseedgermplasmorg/site/339/default.aspx

  10. Feigin A, Rylski I, Shalhevet J (1984) Response of melon plants to different concentration of NO3 and Cl in saline nutrient solutions. In: Paper presented in Soil Salinity under Irrigation–Processes and Management, International Conference, International Soc. Soil Sci. And Israel Soc. Soil SC ii, Bet Dagan, Israel

  11. Fisher RA (1936) The coefficient of racial likeness and the future of craniometry. J R Anthropol Inst Greater Br Irel 66:57–63

  12. Hamidi A, Ghalavand A, Dehghan SM, Malakouti MJ, Choukan R, Asgharzadeh A (2006) The effects of application of plant growth promoting rhizobacteria (PGPR) on the yield of fodder maize (Zea Mays L.). Iran J Agric Sci 37–1(3):493–499

  13. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198

  14. Isayenkov SV, Maathuis FJM (2015) Plant salinity stress: many unanswered questions remain. Front Plant Sci 10:80.


  16. Jamil A, Riaz S, Ashraf MMR (2011) Foolad Gene expression profiling of plants under salt stress. Crit Rev Plant Sci 30(5):435–458

  17. Khodary (2004) Effect of NaCl salinity on improvement of nitrogen metabolism and some ions uptake in lupine plants subjected to gamma irradiation. Int J Agric Biol 6:1–4

  18. Kumar S, Pandey AK (2017) Chemistry and biological activities of flavonoids: an overview. Sci World J 2013:1–16

  19. Laux (2011) Accessed 13 Mar 2012

  20. Maguire JD (1962) Speed of germination—aid in selection and evaluation for seedling emergence and vigor. Crop Sci 2:176–177

  21. Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410

  22. Putter J (1974) Peroxidases. Methods of enzymatic. Analysis 685:690.

  23. Rahneshan Z, Nasibi F, Moghadam AA (2018) Effects of salinity stress on some growth, physiological, biochemical parameters and nutrients in two pistachio (Pistaciavera L.) rootstocks. J Plant Interact 13(1):73–82.

  24. Robin AHK, Matthew C, Uddin MJ, Bayazid KN (2016) Salinity-induced reduction in root surface area and changes in major root and shoot traits at the phytomer level in wheat. J Exp Bot 67(12):3719–3729.

  25. Safarnejad AS, Sadr VA, Hamidi H (2007) Effect of salinity stress on morphological characters of Nigella sativa. Iran J Rangel for Plant Breed Genet Res 15(27):75–84

  26. Salami KD, Odewale MA, Iroko OA (2020) Epicarp response to seeds germination and early growth performance of Spondia spurpurea as affected by different soil media. Fudma J Agric Agric Tech 6(1):119–126

  27. Segatoleslami MJ (2010) Effect of salinity on germination of three species of medicinal savory (Satureja hortensis L.), Chicory (Cynaras colymus L.) and Artichoke (Cichorium intybus L.). Iran J Agric Res 8(5):818–823

  28. Sharma DK (2014) CSSRI Vision 2050. Central Soil Salinity Research Institute, Karnal

  29. Shekhar S, Gambhir G, Padaria JC (2017) Bioprospecting of genes from microbes for stress management in agricultural crops. In: Singh JS, Seneviratne G (eds) Agro-environmental sustainability. Springer International Publishing AG, New York, pp 127–147.

  30. Siddiqi EH, Ashraf M, Fahad Al-QurainycAkramb NA (2011) Saltinduced modulation in inorganic nutrients, antioxidant enzymes, proline content and seed oil composition in safflower (Carthamus tinctorius L.). J Sci Food Agric 91:2785–2793

  31. Singh AK (2004) The physiology of salt tolerance in four genotypes of chickpea during germination. J Agric Sci Technol 6(87):93

  32. Swapna TS (2003) Salt stress induced changes on enzyme activities during different developmental stages of rice. Indian J Biotechnol 2:251–258

  33. Tambhale S, Kumar V, Shriramc V (2011) Differential response of two scented Indica rice (Oryza sativa) cultivars under salt stress. J Stress Physiol Biochem 7(4):387–397



The authors wish to express their sincere gratitude to the Sam Higginbottom University of Agriculture, Technology and Sciences. Thanks to DST Inspire for financial support Atul Singh. Thanks to the Department of Biological Sciences for providing lab facilities to conduct research work.

Author Information

Singh Atul
Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, India

Shekhar Shashi
Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, India

Marker Shailesh
Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, India

Ramteke P. W.
Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, India