Characterization of pathogens associated with the wilt complex of chickpea in Gujarat, India

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
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Doi: 10.1007/s42535-022-00384-5
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Keywords: Chickpea wilt complex, Fusarium oxysporum f. sp. ciceri , Fusarium solani , Macrophomina phaseolina , Survey


Abstract


Soil-borne diseases caused by a complex of various soil fungi are one of the constraints for the production of chickpea in Gujarat, India. The identification and determination regarding the distribution of these fungi in those areas will define the risk of damage to the crop. The disease was mapped and samples were collected from chickpea-growing pockets of Gujarat viz., Anand, Kheda, Dahod and Panchmahal districts, Bhal and Ghed regions during the 2018–19 rabi season. The disease was widespread at all the surveyed locations and the disease incidence varied from 1.80 to 52.55% thereby indicating the importance of the problem. Based on cultural characteristics, morphology and sequence analysis of the Internal Transcribed Spacer (ITS) and TEF-1α gene, the pathogens associated with the wilt complex of chickpea were identified as Fusarium oxysporum f. sp. ciceri, Fusarium solani and Macrophomina phaseolina.


Chickpea wilt complex, 
                        Fusarium oxysporum f. sp. ciceri
                     , 
                Fusarium solani
              , 
                Macrophomina phaseolina
              , Survey


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References


Akbar A, Hussain S, Ullah K, Fahim M, Ali G (2018) Detection, virulence and genetic diversity of Fusarium species infecting tomato in Northern Pakistan. PLoS ONE 13(9):1–21


Andrabi A, Vaid A, Razdan VK (2008) Occurrence of chickpea wilt complex and its causal agents in Jammu. J Res SKUAST-J 7(1):1–8


Ashby SF (1927) Macrophomina phaseolina (Maubl.) Comb. Nov. the pycnidial stage of Rhizoctonia bataticola (Taub.). Butl. Trans Br Mycol Soc 12:141–147


Booth C (1971) The genus Fusarium. Commonwealth Mycological Institute, Kew (Surry), England, p 237


Chehri K (2016) Molecular identification of pathogenic Fusarium species, the causal agents of tomato wilt in Western Iran. J Plant Prot Res 56(2):143–148


Datta S, Choudhary RG, Shamim M, Dhar V (2011) Polymorphism in the internal transcribed spacer (ITS) region of the ribosomal DNA among different Fusarium species. Arch Phytopathol Plant Prot 44(6):558–566


Ghosh R, Sharma M, Telangre R, Pande S (2013) Occurrence and distribution of chickpea diseases in Central and Southern parts of India. Am J Plant Sci 4:940–944


Goidanich G (1947) A revision of the genus Macrophomina Petrak type species: M. P. (Tassi) G. Goid. and M. P. (Maubl.) Ashby. Annali. Sper. Agr. (N. S.), pp 449–461


Jalali BL, Chand H (1992) Chickpea wilt. In: Plant diseases of international importance, vol 1. Diseases of cereals and pulses. In: Singh US, Mukhopadhyay AN, Chaube HS (eds) Englewood Cliffs, New York, pp 429–444


Jibfaakate PH, Mondhe MK, Mate GD (2010) Assessment of wilt complex in chickpea crop at Nagpur district. J Plant Dis Sci 5(2):251–253


Kapoor SK, Sugha SK, Singh BM (1991) Incidence of wilt-like diseases of chickpea (Cicer arietinum) in Himachal Pradesh. Indian J Agric Sci 61(11):853–855


Khan AN, Shair F, Malik K, Hayat Z, Khan MA, Hafeez FY, Hassan MN (2017) Molecular identification and genetic characterization of Macrophomina phaseolina strains causing pathogenicity on sunflower and chickpea. Front Microbiol 8:1308–1314


Leslie JF, Summerell BA (2006) The Fusarium laboratory manual. Blackwell Publishing Professional, State Avenue, Ames


Liu YJ, Whelen S, Hall BD (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Mol Biol Evol 16:1799–1808


Minati MH, Mohammed-Ameen MK (2019) Novel report on six Fusarium species associated with head blight and crown rot of wheat in Basra province, Iraq. Bull Natl Res Centre 43:139–154


Murad NB, Kusai NA, Zainudin NA (2016) Identification and diversity of Fusarium species isolated from tomato fruits. J Plant Prot Res 56(3):231–236


Nene YL, Haware MP, Reddy MV (1981) Chickpea diseases: resistance screening techniques. Inf Bull ICRISAT Patancheru Andhra Pradesh India 10:1–8


Nene YL, Haware MP, Reddy MV, Phillip EL, Castro SR, Kotasthane SR, Singh G, Shulka P, Sah RP (1989) Identification of broad and stable resistance to wilt and root rot in chickpea. Indian Phytopathol 42:499–505


Nene YL, Sheila VK, Sharma SB (1996) A world list of chickpea and pigeonpea pathogens, 5th edn. ICRISAT, Patancheru, p 27


Pande S, Sharma M, Avuthu N, Telangre R (2012) High throughput phenotyping of chickpea diseases: stepwise identification of host plant resistance. Information Bulletin, ICRISAT, Patancheru, p 38


Rangaswami G (1972) Diseases of crop plants in India, 1st edn. Prentice-Hall of India Pvt. Ltd., New Delhi, p 408


Sharma M, Pande S (2013) Unravelling effects of temperature and soil moisture stress response on development of dry root rot [Rhizoctonia bataticola (Taub.)] Butler in chickpea. Am J Plant Sci 4:584–589


Sharma M, Ghosh R, Pande S (2015) Dry root rot (Rhizoctonia bataticola (Taub.) Butler): an emerging disease of chickpea—where do we stand? Arch Phytopathol Plant Prot 48(13–16):797–812


Singh D, Kumar A, Singh AK, Tripathi HS (2013) Severity of chickpea wilt in North Bihar and nutritional studies on Fusarium oxysporum f. sp ciceri. J Plant Dis Sci 8(2):137–140


Suhasini P, Kiresur VR, Rao GDN, Bantilan MCS (2009) Adoption of chickpea cultivars in Andhra Pradesh: pattern, trends and constraints. Monograph. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Andhra Pradesh, India


Tekeoğlu M, Özkılınç H, Tunalı B, Küsmenoğlu İ, Chen W (2017) Molecular identification of Fusarium spp. causing wilt and the first report of Fusarium redolens in Turkey. Mediterr Agric Sci 30(1):27–33


White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego

 


Acknowledgements



Author Information


Parmar H. V.
Department of Plant Pathology, B. A. College of Agriculture, Anand Agricultural University, Anand, India
harshilprmr@gmail.com
Gohel N. M.
Department of Plant Pathology, B. A. College of Agriculture, Anand Agricultural University, Anand, India