Chemical composition of the essential oil and isolation of two main constituents of Mentha pulegium L.

, , , , ,


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-023-00572-x
First Page: 82
Last Page: 93
Views: 1225


Keywords: Mentha pulegium , Essential oil, Isolation, Pulegone, Piperitenone


Abstract


Aromatic and medicinal plants are the reservoirs of a vast quantity of molecules with different activities. Pennyroyal or Mentha pulegium L. is one of the miraculous plants that is very rich in aromatic essence and mostly used for their medicinal virtues. The purpose of the present work is to determine the chemical composition of the essential oil of the flowering tops of pennyroyal, which were harvested during July 2017 in M’Rirt (Middle-Atlas, Morocco), and to separate its constituents. The analysis of the essential oil, extracted by hydrodistillation, was made using gas chromatography coupled with mass spectrometry (GC/MS). The results of this analysis showed that the essential oil of pennyroyal contains two main compounds: pulegone and piperitenone with a rate of 71.97 and 26.04%, respectively. These two molecules represent 98.01% of the identified products. Subsequently, this essence was split on the open column of silica using an elected official of growing polarity in order to isolate certain compounds. Two main constituents were isolated and identified by the analysis of their spectroscopic data.



              Mentha pulegium
            , Essential oil, Isolation, Pulegone, Piperitenone


*Get Access

(*Only SPR Life Members can get full access.)

Advertisement

References


Adams RP (2007) Identification of essential oils components by gas chromatography/mass spectroscopy. 4th Edn. Allured: Carol Stream, IL, pp 804


AFNOR (1996) Huile essentielle, recueil des normes françaises. 5ème édition.1. échantillonnage et méthodes d’analyse, 2.spécifications, AFNOR, Paris


Aid K, Alami I, Benali D, Zemzami M, Mokhtari A, SoulaYmani A (2003) Multiplication massive in vitro de Mentha pulegium. Biologie & Santé 3(2):244–251


Anonyme Source: Direction de la protection de végétaux-Rabat (1999) Ministère de l’Agriculture. Rabat- Maroc


Bellakhder J (1997) Contribution à l’étude de la pharmacopée traditionnelle au Maroc: La situation actuelle, les produits, les sources du savoir, 343p


Chraibi M, Amrani M, Farah A, Bari A, Benziane Ouaritini Z (2018) Étude ethnobotanique sur l’utilisation de Mentha pulegium, Mentha piperita et Pelargonium graveolens au nord du Maroc (Taounate) et évaluation de leur pouvoir antimicrobien. Eur Sci J August, 14, 24, ISSN: 1857–7881 (Print) e—ISSN 1857–7431


El Arch M, Satrani B, Farah A, Bennani L, Boriky D, Fechtal M, Blaghen M, Talbi M (2003) Composition chimique et activités antimicrobienne et insecticide de l’huile essentielle de Mentha rotundifolia du Maroc. Acta Botanica Gallica 150(3):267–274


Guignard JL, et Dupont F (2004) Botanique: Systématique moléculaire, 13éme éd. Masson, Paris, pp 237


Kiemele DJ, Silvertein RM, Webster FX (2016) Identification spectrométrique de composés organiques. 3ème édition. De Boeck Superieur, pp 480


Kovats E (1965) Gas chromatographic characterization of organic substances in the retention index system. Adv Chromatogr 1:229–247


Lahsissene H, Kahouadji A (2010) Analyse ethnobotanique des plantes médicinales et aromatiques de la flore marocaine: cas de la région de Zaër. Phytothérapie 8:202–209


Mohammadhosseini M, Venditti A, Mahdavi B (2021) Characterization of essential oils and volatiles from the aerial parts of Mentha pulegium L. (Lamiaceae) using microwave-assisted hydrodistillation (MAHD) and headspace solid phase microextraction (HS-SPME) in combination with GC-MS. Nat Product Res, 1–5


Mollaei S, Ebadi M, Hazrati S, Habibi B, Gholami F, Sourestani MM (2020) Essential oil variation and antioxidant capacity of Mentha pulegium populations and their relation to ecological factors. Biochem Syst Ecol 91:104084


Mollaeia S, Ebadib M, Hazratic S, Habibid B, Gholamia F, Sourestanie MM (2021) Essential oil variation and antioxidant capacity of Mentha pulegium populations and their relation to ecological factors. Biochem Syst Ecol 91(2020):104084


Newall C (1996) Herbal medicines. A guide for health care professionals. In: Anderson LA, Philipson JD (eds). The Pharmaceutical Press, London, pp 296


Ouakouak H, Chohra M, Denane M (2015) Chemical composition, antioxidant activities of the essential oil of Mentha pulegium L, South East of Algeria. Int Lett Nat Sci 39:49–55


Quézel P. et Santa S (1962) Nouvelle flore de l’Algérie et des régions désertiques méridionales. éd. CNRS, Paris, 1, 1‐565


Salhi S, Fadli M, Zidane L. et Douira A (2010) Etudes floristique et ethnobotanique des plantes médicinales de la ville de Kénitra (Maroc) LAZAROA 31: 133–146. https://doi.org/10.5209/rev_LAZA.2010.v31.9 ISSN: 0210-9778


Sarikurkcu C, Eryigit F, Cengiz M, Tepe B, Cakir A, Mete E (2012) Screening of the antioxidant activity of the essential oil and methanol extract of Mentha pulegium L. From Turkey. Spectroscopy Lett 45:352–358


Silva LF, das Graças CM, Batista LR, de Souza GM, Rodrigues LMA, de Rezende DACS, Teixeira ML, Carvalho MSS, de Andrade SJ, Nelson DL (2015) Chemical characterization, antibacterial and antioxidant activities of essential oils of Mentha viridis L. and Mentha pulegium L. (L). Am J Plant Sci 6:666–675


Teixeiraa B, Marques A, Ramos C, Batista I, Serrano C, Matos O, Neng NR, Nogueira JMF, Alexandre SJ, Leonor NM (2012) European pennyroyal (Mentha pulegium) from Portugal: chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil. Indus Crops Products 36:81–87


Zantar S, Haouzi R, Chabbi M, Laglaoui A, Mouhib M, Boujnah M, Zerrouk MH (2015) Effect of gamma irradiation on chemical composition, antimicrobial and antioxidant activities of Thymus vulgaris and Mentha pulegium essential oils. Radiation Phys Chem 115:6–11


Zargari A (1990) Herbal medicines. Publication of Tehran University, Tehran

 


Acknowledgements


We are grateful to Mr M. Ibn Tattou, Professor at the Scientific Institute of Rabat, for the species identification


Author Information


Amalich Smail
Laboratory of Phytochemistry, National Agency of Medicinal and Aromatic Plants, Taounate, Morocco
amalich80@gmail.com
Zerkani Hannou
Research Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, Meknes, Morocco


Tagnaout Imane
Research Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, Meknes, Morocco


Ali Cherrat
Research Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, Meknes, Morocco


Kamal Fadili
Research Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, Meknes, Morocco

Zair Touria
Research Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, Meknes, Morocco