Colonization of Endophytic Bacterium Pantoea ananatis MR5 Strain by Tagging with GFP in Wheat Tissues

Akbari Disheeta L.*, Parkhiya M. V., Bhadania Roshani A., Akbari L. F.,


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.5958/2229-4473.2016.00047.1
First Page: 142
Last Page: 146
Views: 1224


Keywords: Cenchrus biflorus, Pantoea ananatis MR5, Endophyte, GFP, Wheat.


Abstract


The strain, Pantoea ananatis MR5 taxonomically identified using 16S ribosomal DNA sequence was isolated from roots of Cenchrus biflorus. Its endophytic colonization was investigated microscopically using green fluorescent protein introduced by vector pGLO. The strain entered wheat tissues through the root and was localized in the roots, stems & leaves.


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References



Acknowledgements



Author Information


Akbari Disheeta L.* Parkhiya M. V. Bhadania Roshani A. Akbari L. F.
Department of Biotechnology, College of Agriculture, Junagadh Agriculture University, Junagadh-362001

*Corresponding author E-mail: disheetaakbari@gmail.com