Comparative study on detection efficacy of universal potyviruses primers

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-021-00254-6
First Page: 745
Last Page: 749
Views: 537


Keywords: ELISA, Nucleic acid based assay, Vegetables


Abstract


Four universal primer pairs were tested and compared for specificity and efficiency of detection of diverse potyviruses in vegetable crops. Symptomatic samples of seven vegetable crops were initially exposed to DAS/TAS-ELISA. After initial serological detection positive samples were further exposed to different universal primer pairs. All the primers correspond to most conserved regions/gene of potyvirus genome viz., Nuclear Inclusion B and coat protein. Out of four pairs tested, most potential and reproducible candidate universal primer for detection was NIb2F/3R. This set of primer has great breadth of specificity as compared to others, as it was able to detect six different potyviruses. Comparative success to detect potyviruses was maximum with primers pair NIb2F/NIb3R i.e. 84%, 22 and 20% with P9502/CPUP and Sprimer/M4T, respectively.

ELISA, Nucleic acid based assay, Vegetables


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Acknowledgements



Author Information


Abhishek Sharma
Department of Vegetable Science, College of Horticulture and Forestry, Punjab Agricultural University, Ludhiana, India

Irfan Khan
Department of Plant Pathology, College of Agriculture, Punjab Agricultural University, Ludhiana, India


Sukhjeet Kaur
Department of Vegetable Science, College of Horticulture and Forestry, Punjab Agricultural University, Ludhiana, India


Sumit Inder Kaur
Department of Plant Pathology, College of Agriculture, Punjab Agricultural University, Ludhiana, India