Vegetos
(An International Journal of Plant Research & Biotechnology)
(eISSN: 2229-4473)
Computational insights into the glutathione S-transferase gene family in grain amaranths (Amaranthus caudatus L. and Amaranthus cruentus L.)
*Article not assigned to an issue yet
Research Articles | Published: 17 August, 2026
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Keywords: Amaranthus, Glutathione S-transferase, Genome-wide analysis, Abiotic stress, Gene duplication, Expression profiling
Abstract
Glutathione S-transferases (GSTs) are a large, multifunctional superfamily of enzymes central to cellular detoxification, redox homeostasis and abiotic-stress tolerance in plants. Grain amaranths are highly nutritious, climate-resilient pseudocereals, yet their GST repertoire has remained uncharacterized. Here we present the first genome-wide identification and comparative analysis of the GST gene family in two cultivated grain amaranths, Amaranthus caudatus and Amaranthus cruentus. Using the annotated A. cruentus reference proteome and de-novo protein-to-genome prediction on the chromosome-level A. caudatus assembly (GCA_055266045.1), 45 AcrGST and 35 AcGST genes were identified and assigned to ten classes, with Tau and Phi being the most abundant in both species. The predicted proteins were largely acidic to neutral, hydrophilic (all GRAVY< 0) and cytosolic, with a small plastidic component. The genes were unevenly distributed across the chromosomes and displayed conserved class-specific exon–intron architecture (mean ~4 coding exons) and conserved motifs. Gene-duplication analysis identified both tandem and segmental duplications, and all duplicated pairs evolved under purifying selection (Ka/Ks 0.026–0.442, all <1). Promoter regions were enriched in stress and hormone-responsive cis-elements. Cross-species, ortholog-based expression profiling using A. hypochondriacus leaf transcriptomes revealed that several GST members were transcriptionally responsive to salinity and drought, with salinity eliciting the broader response. Collectively, these results provide a verified, fully reproducible framework of the amaranth GST family and candidate genes for abiotic-stress improvement of these underutilized crops.
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Author Information
Department of Integrated and Advanced Technology, School of Engineering and Sciences, Sir Padampat Singhania University, Udaipur, India